Experiment: Erythromycin 12.5 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu0686 and Atu0687 are separated by 151 nucleotides Atu0687 and Atu0688 are separated by 54 nucleotides Atu0688 and Atu0689 are separated by 71 nucleotides
Atu0686: Atu0686 - isoleucyl-tRNA synthetase, at 680,863 to 683,766
Atu0686
Atu0687: Atu0687 - hypothetical protein, at 683,918 to 684,547
Atu0687
Atu0688: Atu0688 - cytidine and deoxycytidylate deaminase, at 684,602 to 685,060
Atu0688
Atu0689: Atu0689 - ribosomal large subunit pseudouridine synthase B, at 685,132 to 687,141
Atu0689
Position (kb) 684
685
686 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 683.935 kb on + strand at 684.162 kb on - strand, within Atu0687 at 684.352 kb on + strand, within Atu0687 at 684.588 kb on - strand at 685.075 kb on + strand at 685.106 kb on - strand at 685.116 kb on - strand at 685.242 kb on + strand at 685.250 kb on - strand at 685.359 kb on - strand, within Atu0689 at 685.482 kb on + strand, within Atu0689 at 685.668 kb on + strand, within Atu0689 at 685.729 kb on + strand, within Atu0689 at 685.799 kb on + strand, within Atu0689 at 685.837 kb on - strand, within Atu0689 at 685.886 kb on + strand, within Atu0689 at 685.973 kb on + strand, within Atu0689
Per-strain Table
Position Strand Gene LocusTag Fraction Erythromycin 12.5 ug/mL remove 683,935 + +1.1 684,162 - Atu0687 0.39 -1.7 684,352 + Atu0687 0.69 -3.5 684,588 - +1.6 685,075 + +0.6 685,106 - +0.8 685,116 - +2.2 685,242 + +3.1 685,250 - +3.5 685,359 - Atu0689 0.11 +2.3 685,482 + Atu0689 0.17 +2.1 685,668 + Atu0689 0.27 +3.0 685,729 + Atu0689 0.30 +2.8 685,799 + Atu0689 0.33 +2.6 685,837 - Atu0689 0.35 +4.6 685,886 + Atu0689 0.38 +3.5 685,973 + Atu0689 0.42 +2.7
Or see this region's nucleotide sequence