Experiment: Erythromycin 12.5 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu0085 and Atu0086 are separated by 3 nucleotides Atu0086 and Atu0087 are separated by 75 nucleotides Atu0087 and Atu0088 are separated by 102 nucleotides Atu0088 and Atu0089 overlap by 4 nucleotides
Atu0085: Atu0085 - tRNA pseudouridine 55 synthase, at 87,307 to 88,254
Atu0085
Atu0086: Atu0086 - ribosome-binding factor A, at 88,258 to 88,674
Atu0086
Atu0087: Atu0087 - translation initiation factor IF-2, at 88,750 to 91,491
Atu0087
Atu0088: Atu0088 - hypothetical protein, at 91,594 to 92,295
Atu0088
Atu0089: Atu0089 - N-utilization substance protein A, at 92,292 to 93,908
Atu0089
Position (kb) 88
89
90
91
92 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 87.751 kb on + strand, within Atu0085 at 87.832 kb on - strand, within Atu0085 at 87.862 kb on - strand, within Atu0085 at 88.213 kb on + strand at 88.221 kb on - strand at 88.221 kb on - strand at 88.221 kb on - strand at 88.221 kb on - strand at 88.456 kb on + strand, within Atu0086 at 88.625 kb on - strand, within Atu0086 at 88.768 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Erythromycin 12.5 ug/mL remove 87,751 + Atu0085 0.47 +0.3 87,832 - Atu0085 0.55 +2.9 87,862 - Atu0085 0.59 +4.3 88,213 + +2.3 88,221 - +2.3 88,221 - +2.9 88,221 - +3.3 88,221 - +2.9 88,456 + Atu0086 0.47 -0.3 88,625 - Atu0086 0.88 -2.0 88,768 - -1.3
Or see this region's nucleotide sequence