Experiment: Erythromycin 12.5 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu0009 and Atu0010 overlap by 4 nucleotides Atu0010 and Atu0011 overlap by 4 nucleotides Atu0011 and Atu0012 are separated by 158 nucleotides
Atu0009: Atu0009 - membrane-bound lytic murein transglycosylase, at 5,865 to 6,977
Atu0009
Atu0010: Atu0010 - hypothetical protein, at 6,974 to 7,543
Atu0010
Atu0011: Atu0011 - transcriptional regulator, at 7,540 to 7,905
Atu0011
Atu0012: Atu0012 - DNA gyrase subunit B, at 8,064 to 10,499
Atu0012
Position (kb)
6
7
8 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 5.990 kb on - strand, within Atu0009 at 6.003 kb on + strand, within Atu0009 at 6.055 kb on - strand, within Atu0009 at 6.098 kb on + strand, within Atu0009 at 6.165 kb on - strand, within Atu0009 at 6.172 kb on + strand, within Atu0009 at 6.224 kb on - strand, within Atu0009 at 6.289 kb on - strand, within Atu0009 at 6.289 kb on - strand, within Atu0009 at 6.435 kb on + strand, within Atu0009 at 6.435 kb on + strand, within Atu0009 at 6.527 kb on - strand, within Atu0009 at 6.538 kb on - strand, within Atu0009 at 6.660 kb on + strand, within Atu0009 at 6.710 kb on - strand, within Atu0009 at 6.742 kb on + strand, within Atu0009 at 6.801 kb on - strand, within Atu0009 at 6.827 kb on + strand, within Atu0009 at 6.920 kb on - strand at 7.026 kb on - strand at 7.098 kb on + strand, within Atu0010 at 7.148 kb on + strand, within Atu0010 at 7.200 kb on + strand, within Atu0010 at 7.200 kb on - strand, within Atu0010 at 7.208 kb on - strand, within Atu0010 at 7.228 kb on + strand, within Atu0010 at 7.271 kb on + strand, within Atu0010 at 7.334 kb on + strand, within Atu0010 at 7.363 kb on - strand, within Atu0010 at 7.378 kb on - strand, within Atu0010 at 7.378 kb on - strand, within Atu0010 at 7.378 kb on - strand, within Atu0010 at 7.378 kb on - strand, within Atu0010 at 7.405 kb on + strand, within Atu0010 at 7.410 kb on + strand, within Atu0010 at 7.423 kb on - strand, within Atu0010 at 7.761 kb on + strand, within Atu0011 at 7.800 kb on + strand, within Atu0011 at 7.812 kb on + strand, within Atu0011 at 7.866 kb on + strand, within Atu0011 at 7.910 kb on + strand at 7.922 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Erythromycin 12.5 ug/mL remove 5,990 - Atu0009 0.11 -1.5 6,003 + Atu0009 0.12 -0.3 6,055 - Atu0009 0.17 -0.1 6,098 + Atu0009 0.21 -1.2 6,165 - Atu0009 0.27 -0.9 6,172 + Atu0009 0.28 -1.1 6,224 - Atu0009 0.32 -1.7 6,289 - Atu0009 0.38 +0.8 6,289 - Atu0009 0.38 -1.2 6,435 + Atu0009 0.51 -0.7 6,435 + Atu0009 0.51 -1.2 6,527 - Atu0009 0.59 +0.1 6,538 - Atu0009 0.60 -1.0 6,660 + Atu0009 0.71 -0.3 6,710 - Atu0009 0.76 -2.2 6,742 + Atu0009 0.79 -2.7 6,801 - Atu0009 0.84 -1.7 6,827 + Atu0009 0.86 -0.1 6,920 - -1.0 7,026 - +0.3 7,098 + Atu0010 0.22 -0.7 7,148 + Atu0010 0.31 -1.1 7,200 + Atu0010 0.40 +0.7 7,200 - Atu0010 0.40 -1.3 7,208 - Atu0010 0.41 -1.3 7,228 + Atu0010 0.45 -2.2 7,271 + Atu0010 0.52 -0.3 7,334 + Atu0010 0.63 -0.8 7,363 - Atu0010 0.68 -2.4 7,378 - Atu0010 0.71 -1.7 7,378 - Atu0010 0.71 -0.9 7,378 - Atu0010 0.71 -0.9 7,378 - Atu0010 0.71 -3.6 7,405 + Atu0010 0.76 -0.3 7,410 + Atu0010 0.76 -1.7 7,423 - Atu0010 0.79 -2.2 7,761 + Atu0011 0.60 -0.3 7,800 + Atu0011 0.71 -1.3 7,812 + Atu0011 0.74 +0.0 7,866 + Atu0011 0.89 -0.2 7,910 + +0.5 7,922 + +1.1
Or see this region's nucleotide sequence