Experiment: Erythromycin 6.25 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu3359 and Atu3360 are separated by 12 nucleotides Atu3360 and Atu3361 are separated by 4 nucleotides Atu3361 and Atu3362 are separated by 26 nucleotides
Atu3359: Atu3359 - amino acid ABC transporter permease, at 397,043 to 397,825
Atu3359
Atu3360: Atu3360 - amino acid ABC transporter ATPase/permease, at 397,838 to 398,653
Atu3360
Atu3361: Atu3361 - hypothetical protein, at 398,658 to 400,004
Atu3361
Atu3362: Atu3362 - monooxygenase, at 400,031 to 401,341
Atu3362
Position (kb) 398
399
400
401 Strain fitness (log2 ratio) -2
-1
0
1
2 at 397.754 kb on + strand at 397.824 kb on + strand at 397.832 kb on - strand at 397.848 kb on - strand at 397.848 kb on - strand at 397.848 kb on - strand at 397.861 kb on - strand at 397.900 kb on + strand at 397.933 kb on + strand, within Atu3360 at 397.944 kb on - strand, within Atu3360 at 398.015 kb on + strand, within Atu3360 at 398.023 kb on - strand, within Atu3360 at 398.067 kb on - strand, within Atu3360 at 398.079 kb on + strand, within Atu3360 at 398.087 kb on - strand, within Atu3360 at 398.270 kb on - strand, within Atu3360 at 398.330 kb on + strand, within Atu3360 at 398.338 kb on - strand, within Atu3360 at 398.369 kb on - strand, within Atu3360 at 398.515 kb on - strand, within Atu3360 at 398.540 kb on + strand, within Atu3360 at 398.553 kb on - strand, within Atu3360 at 398.948 kb on - strand, within Atu3361 at 398.948 kb on - strand, within Atu3361 at 398.948 kb on - strand, within Atu3361 at 398.948 kb on - strand, within Atu3361 at 398.960 kb on + strand, within Atu3361 at 398.987 kb on + strand, within Atu3361 at 399.005 kb on - strand, within Atu3361 at 399.029 kb on - strand, within Atu3361 at 399.096 kb on - strand, within Atu3361 at 399.155 kb on - strand, within Atu3361 at 399.167 kb on + strand, within Atu3361 at 399.268 kb on + strand, within Atu3361 at 399.268 kb on + strand, within Atu3361 at 399.281 kb on - strand, within Atu3361 at 399.395 kb on + strand, within Atu3361 at 399.426 kb on + strand, within Atu3361 at 399.496 kb on - strand, within Atu3361 at 399.596 kb on - strand, within Atu3361 at 399.702 kb on + strand, within Atu3361 at 399.720 kb on - strand, within Atu3361 at 399.778 kb on + strand, within Atu3361 at 399.783 kb on + strand, within Atu3361 at 399.786 kb on - strand, within Atu3361 at 399.938 kb on + strand at 399.946 kb on - strand at 399.972 kb on - strand at 400.014 kb on + strand at 400.035 kb on - strand at 400.091 kb on - strand at 400.158 kb on - strand at 400.345 kb on + strand, within Atu3362 at 400.353 kb on - strand, within Atu3362 at 400.408 kb on - strand, within Atu3362 at 400.408 kb on - strand, within Atu3362 at 400.453 kb on + strand, within Atu3362 at 400.575 kb on - strand, within Atu3362 at 400.589 kb on + strand, within Atu3362 at 400.618 kb on - strand, within Atu3362 at 400.644 kb on - strand, within Atu3362 at 400.663 kb on - strand, within Atu3362 at 400.700 kb on + strand, within Atu3362 at 400.700 kb on + strand, within Atu3362 at 400.763 kb on + strand, within Atu3362 at 400.782 kb on + strand, within Atu3362 at 400.782 kb on - strand, within Atu3362 at 400.944 kb on - strand, within Atu3362 at 400.952 kb on + strand, within Atu3362 at 400.967 kb on - strand, within Atu3362 at 400.990 kb on + strand, within Atu3362 at 401.004 kb on + strand, within Atu3362
Per-strain Table
Position Strand Gene LocusTag Fraction Erythromycin 6.25 ug/mL remove 397,754 + +0.6 397,824 + +0.3 397,832 - -0.1 397,848 - -0.2 397,848 - +0.8 397,848 - -0.3 397,861 - -0.1 397,900 + -0.4 397,933 + Atu3360 0.12 +0.2 397,944 - Atu3360 0.13 -0.5 398,015 + Atu3360 0.22 +0.1 398,023 - Atu3360 0.23 +2.0 398,067 - Atu3360 0.28 -0.1 398,079 + Atu3360 0.30 -1.8 398,087 - Atu3360 0.31 +0.0 398,270 - Atu3360 0.53 +0.1 398,330 + Atu3360 0.60 -0.2 398,338 - Atu3360 0.61 -0.4 398,369 - Atu3360 0.65 -0.6 398,515 - Atu3360 0.83 -0.8 398,540 + Atu3360 0.86 -1.8 398,553 - Atu3360 0.88 -1.2 398,948 - Atu3361 0.22 -1.1 398,948 - Atu3361 0.22 -0.5 398,948 - Atu3361 0.22 -0.4 398,948 - Atu3361 0.22 -2.0 398,960 + Atu3361 0.22 -0.1 398,987 + Atu3361 0.24 +0.1 399,005 - Atu3361 0.26 -0.3 399,029 - Atu3361 0.28 +0.6 399,096 - Atu3361 0.33 +0.0 399,155 - Atu3361 0.37 +0.0 399,167 + Atu3361 0.38 -0.1 399,268 + Atu3361 0.45 -0.1 399,268 + Atu3361 0.45 -1.0 399,281 - Atu3361 0.46 -0.4 399,395 + Atu3361 0.55 -1.6 399,426 + Atu3361 0.57 +0.5 399,496 - Atu3361 0.62 +0.6 399,596 - Atu3361 0.70 -0.1 399,702 + Atu3361 0.78 +0.1 399,720 - Atu3361 0.79 -0.8 399,778 + Atu3361 0.83 -0.8 399,783 + Atu3361 0.84 -2.0 399,786 - Atu3361 0.84 -0.2 399,938 + -0.1 399,946 - +0.8 399,972 - -0.2 400,014 + +0.2 400,035 - -1.4 400,091 - -0.1 400,158 - -0.2 400,345 + Atu3362 0.24 +0.2 400,353 - Atu3362 0.25 -0.9 400,408 - Atu3362 0.29 +0.9 400,408 - Atu3362 0.29 -0.2 400,453 + Atu3362 0.32 -0.7 400,575 - Atu3362 0.41 -0.8 400,589 + Atu3362 0.43 -0.9 400,618 - Atu3362 0.45 -0.4 400,644 - Atu3362 0.47 -0.2 400,663 - Atu3362 0.48 -0.3 400,700 + Atu3362 0.51 +1.2 400,700 + Atu3362 0.51 +0.1 400,763 + Atu3362 0.56 -0.2 400,782 + Atu3362 0.57 -0.0 400,782 - Atu3362 0.57 +0.4 400,944 - Atu3362 0.70 +0.1 400,952 + Atu3362 0.70 -0.4 400,967 - Atu3362 0.71 +0.3 400,990 + Atu3362 0.73 -0.4 401,004 + Atu3362 0.74 +1.8
Or see this region's nucleotide sequence