Experiment: Erythromycin 6.25 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu2299 and Atu2300 are separated by 208 nucleotides Atu2300 and Atu2301 are separated by 133 nucleotides Atu2301 and Atu2302 are separated by 155 nucleotides
Atu2299: Atu2299 - hypothetical protein, at 2,274,151 to 2,274,759
Atu2299
Atu2300: Atu2300 - GTP-binding protein, at 2,274,968 to 2,276,398
Atu2300
Atu2301: Atu2301 - hypothetical protein, at 2,276,532 to 2,277,203
Atu2301
Atu2302: Atu2302 - hypothetical protein, at 2,277,359 to 2,277,943
Atu2302
Position (kb)
2274
2275
2276
2277 Strain fitness (log2 ratio)
-1
0
1 at 2274.027 kb on + strand at 2274.037 kb on + strand at 2274.037 kb on + strand at 2274.045 kb on - strand at 2274.438 kb on + strand, within Atu2299 at 2274.441 kb on + strand, within Atu2299 at 2274.570 kb on - strand, within Atu2299 at 2274.602 kb on + strand, within Atu2299 at 2274.622 kb on - strand, within Atu2299 at 2274.623 kb on + strand, within Atu2299 at 2274.623 kb on - strand, within Atu2299 at 2274.763 kb on + strand at 2274.845 kb on + strand at 2274.860 kb on + strand at 2274.871 kb on + strand at 2276.537 kb on - strand at 2276.632 kb on - strand, within Atu2301 at 2277.308 kb on - strand at 2277.308 kb on - strand at 2277.373 kb on + strand at 2277.373 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Erythromycin 6.25 ug/mL remove 2,274,027 + +0.1 2,274,037 + -0.7 2,274,037 + -0.1 2,274,045 - +0.5 2,274,438 + Atu2299 0.47 +0.8 2,274,441 + Atu2299 0.48 -0.1 2,274,570 - Atu2299 0.69 -1.0 2,274,602 + Atu2299 0.74 +0.3 2,274,622 - Atu2299 0.77 +0.2 2,274,623 + Atu2299 0.78 +1.3 2,274,623 - Atu2299 0.78 +0.4 2,274,763 + +0.3 2,274,845 + +0.6 2,274,860 + -0.7 2,274,871 + -0.4 2,276,537 - +0.5 2,276,632 - Atu2301 0.15 +0.9 2,277,308 - +0.1 2,277,308 - -0.4 2,277,373 + +0.5 2,277,373 + -0.0
Or see this region's nucleotide sequence