Experiment: Erythromycin 6.25 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu1657 and Atu1658 are separated by 369 nucleotides Atu1658 and Atu1659 overlap by 1 nucleotides Atu1659 and Atu1660 are separated by 109 nucleotides
Atu1657: Atu1657 - NAD (+) synthetase, at 1,641,644 to 1,643,323
Atu1657
Atu1658: Atu1658 - conserved protein involved in phosphoglycerol modification of cyclic glucan, at 1,643,693 to 1,645,486
Atu1658
Atu1659: Atu1659 - hypothetical protein, at 1,645,486 to 1,646,136
Atu1659
Atu1660: Atu1660 - hypothetical protein, at 1,646,246 to 1,646,785
Atu1660
Position (kb)
1643
1644
1645
1646 Strain fitness (log2 ratio)
-2
-1
0
1 at 1643.336 kb on - strand at 1643.336 kb on - strand at 1643.408 kb on + strand at 1643.409 kb on + strand at 1643.414 kb on + strand at 1643.430 kb on + strand at 1643.610 kb on - strand at 1643.648 kb on + strand at 1643.656 kb on - strand at 1643.687 kb on - strand at 1643.749 kb on - strand at 1643.758 kb on - strand at 1643.834 kb on - strand at 1643.834 kb on - strand at 1643.922 kb on - strand, within Atu1658 at 1643.979 kb on - strand, within Atu1658 at 1644.035 kb on - strand, within Atu1658 at 1644.087 kb on - strand, within Atu1658 at 1644.087 kb on - strand, within Atu1658 at 1644.092 kb on - strand, within Atu1658 at 1644.246 kb on - strand, within Atu1658 at 1644.253 kb on - strand, within Atu1658 at 1644.291 kb on - strand, within Atu1658 at 1644.292 kb on - strand, within Atu1658 at 1644.296 kb on + strand, within Atu1658 at 1644.435 kb on + strand, within Atu1658 at 1644.461 kb on - strand, within Atu1658 at 1644.791 kb on - strand, within Atu1658 at 1644.818 kb on + strand, within Atu1658 at 1644.998 kb on - strand, within Atu1658 at 1645.111 kb on + strand, within Atu1658 at 1645.111 kb on + strand, within Atu1658 at 1645.142 kb on - strand, within Atu1658 at 1645.192 kb on + strand, within Atu1658 at 1645.338 kb on - strand at 1645.456 kb on - strand at 1645.489 kb on - strand at 1645.489 kb on - strand at 1645.510 kb on + strand at 1645.510 kb on + strand at 1645.582 kb on + strand, within Atu1659 at 1645.612 kb on + strand, within Atu1659 at 1645.633 kb on + strand, within Atu1659 at 1645.674 kb on - strand, within Atu1659 at 1645.679 kb on + strand, within Atu1659 at 1645.687 kb on - strand, within Atu1659 at 1645.687 kb on - strand, within Atu1659 at 1645.790 kb on + strand, within Atu1659 at 1645.838 kb on - strand, within Atu1659 at 1645.850 kb on + strand, within Atu1659 at 1645.850 kb on + strand, within Atu1659 at 1645.850 kb on + strand, within Atu1659 at 1645.850 kb on + strand, within Atu1659 at 1645.850 kb on + strand, within Atu1659 at 1645.850 kb on + strand, within Atu1659 at 1645.858 kb on - strand, within Atu1659 at 1645.871 kb on + strand, within Atu1659 at 1645.977 kb on - strand, within Atu1659 at 1646.018 kb on - strand, within Atu1659 at 1646.062 kb on - strand, within Atu1659 at 1646.180 kb on + strand at 1646.188 kb on - strand at 1646.188 kb on - strand at 1646.220 kb on - strand at 1646.248 kb on - strand at 1646.299 kb on - strand at 1646.375 kb on - strand, within Atu1660 at 1646.481 kb on + strand, within Atu1660
Per-strain Table
Position Strand Gene LocusTag Fraction Erythromycin 6.25 ug/mL remove 1,643,336 - +1.3 1,643,336 - +1.3 1,643,408 + +0.3 1,643,409 + -0.4 1,643,414 + +1.2 1,643,430 + +0.6 1,643,610 - -1.0 1,643,648 + -1.6 1,643,656 - -1.1 1,643,687 - -1.5 1,643,749 - -0.4 1,643,758 - -1.2 1,643,834 - -0.3 1,643,834 - +1.3 1,643,922 - Atu1658 0.13 -0.8 1,643,979 - Atu1658 0.16 -2.1 1,644,035 - Atu1658 0.19 +0.1 1,644,087 - Atu1658 0.22 -1.7 1,644,087 - Atu1658 0.22 -1.1 1,644,092 - Atu1658 0.22 -0.2 1,644,246 - Atu1658 0.31 -0.2 1,644,253 - Atu1658 0.31 +0.8 1,644,291 - Atu1658 0.33 -0.2 1,644,292 - Atu1658 0.33 -0.2 1,644,296 + Atu1658 0.34 +0.3 1,644,435 + Atu1658 0.41 -0.7 1,644,461 - Atu1658 0.43 +0.3 1,644,791 - Atu1658 0.61 -1.0 1,644,818 + Atu1658 0.63 -0.6 1,644,998 - Atu1658 0.73 -1.5 1,645,111 + Atu1658 0.79 +0.8 1,645,111 + Atu1658 0.79 -2.2 1,645,142 - Atu1658 0.81 +0.3 1,645,192 + Atu1658 0.84 -1.2 1,645,338 - -0.7 1,645,456 - -0.5 1,645,489 - +0.8 1,645,489 - +0.1 1,645,510 + -0.4 1,645,510 + +0.3 1,645,582 + Atu1659 0.15 -0.3 1,645,612 + Atu1659 0.19 +0.6 1,645,633 + Atu1659 0.23 -0.2 1,645,674 - Atu1659 0.29 -0.2 1,645,679 + Atu1659 0.30 +0.1 1,645,687 - Atu1659 0.31 -1.5 1,645,687 - Atu1659 0.31 -0.2 1,645,790 + Atu1659 0.47 -0.4 1,645,838 - Atu1659 0.54 +0.4 1,645,850 + Atu1659 0.56 -0.1 1,645,850 + Atu1659 0.56 -0.0 1,645,850 + Atu1659 0.56 +0.9 1,645,850 + Atu1659 0.56 -1.2 1,645,850 + Atu1659 0.56 +0.1 1,645,850 + Atu1659 0.56 +0.2 1,645,858 - Atu1659 0.57 -0.0 1,645,871 + Atu1659 0.59 -0.9 1,645,977 - Atu1659 0.75 -0.8 1,646,018 - Atu1659 0.82 +0.8 1,646,062 - Atu1659 0.88 -1.8 1,646,180 + +0.9 1,646,188 - -1.0 1,646,188 - +0.4 1,646,220 - -0.0 1,646,248 - -0.4 1,646,299 - -0.2 1,646,375 - Atu1660 0.24 +1.3 1,646,481 + Atu1660 0.44 -0.7
Or see this region's nucleotide sequence