Experiment: Erythromycin 6.25 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu0614 and Atu0616 are separated by 317 nucleotides Atu0616 and Atu0619 are separated by 208 nucleotides Atu0619 and Atu0620 overlap by 4 nucleotides Atu0620 and Atu0621 are separated by 0 nucleotides
Atu0614: Atu0614 - two component sensor kinase, at 602,711 to 606,538
Atu0614
Atu0616: Atu0616 - tRNA-His, at 606,856 to 606,932
Atu0616
Atu0619: Atu0619 - exopolyphosphatase, at 607,141 to 608,841
Atu0619
Atu0620: Atu0620 - cell division protein, at 608,838 to 609,578
Atu0620
Atu0621: Atu0621 - MFS permease, at 609,579 to 610,976
Atu0621
Position (kb)
607
608
609 Strain fitness (log2 ratio)
-1
0
1 at 606.750 kb on + strand at 607.006 kb on - strand at 607.034 kb on + strand at 607.034 kb on + strand at 607.042 kb on - strand at 607.046 kb on + strand at 607.046 kb on + strand at 607.046 kb on + strand at 607.054 kb on - strand at 607.054 kb on - strand at 607.126 kb on + strand at 607.138 kb on + strand at 607.185 kb on + strand at 609.523 kb on - strand at 609.625 kb on + strand at 609.625 kb on + strand at 609.625 kb on + strand at 609.690 kb on + strand at 609.716 kb on + strand at 609.782 kb on + strand, within Atu0621 at 609.790 kb on - strand, within Atu0621 at 609.811 kb on - strand, within Atu0621 at 609.840 kb on - strand, within Atu0621
Per-strain Table
Position Strand Gene LocusTag Fraction Erythromycin 6.25 ug/mL remove 606,750 + +0.1 607,006 - -0.6 607,034 + -0.1 607,034 + -0.3 607,042 - -0.3 607,046 + -0.6 607,046 + +0.7 607,046 + -0.8 607,054 - +0.2 607,054 - -0.2 607,126 + -0.9 607,138 + +0.8 607,185 + -0.3 609,523 - +0.3 609,625 + +1.1 609,625 + +0.1 609,625 + -0.2 609,690 + -0.4 609,716 + -1.0 609,782 + Atu0621 0.15 +0.3 609,790 - Atu0621 0.15 -0.3 609,811 - Atu0621 0.17 -0.0 609,840 - Atu0621 0.19 +0.2
Or see this region's nucleotide sequence