Experiment: Erythromycin 6.25 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu0147 and Atu0148 are separated by 83 nucleotides Atu0148 and Atu0149 are separated by 184 nucleotides Atu0149 and Atu0150 are separated by 6 nucleotides
Atu0147: Atu0147 - hypothetical protein, at 156,090 to 156,596
Atu0147
Atu0148: Atu0148 - Atu0148-1 mutant of a conserved hypothetical protein, at 156,680 to 157,276
Atu0148
Atu0149: Atu0149 - enoyl-(acyl-carrier-protein) reductase, at 157,461 to 158,267
Atu0149
Atu0150: Atu0150 - 3-oxoacyl-(acyl-carrier-protein) synthase, at 158,274 to 159,497
Atu0150
Position (kb) 157
158
159 Strain fitness (log2 ratio) -2
-1
0
1
2 at 156.530 kb on + strand, within Atu0147 at 156.665 kb on + strand at 156.759 kb on - strand, within Atu0148 at 156.841 kb on - strand, within Atu0148 at 157.215 kb on + strand, within Atu0148 at 157.260 kb on + strand at 157.291 kb on + strand at 157.482 kb on - strand at 157.482 kb on - strand at 157.482 kb on - strand at 157.561 kb on + strand, within Atu0149 at 157.588 kb on - strand, within Atu0149 at 157.795 kb on + strand, within Atu0149 at 157.828 kb on + strand, within Atu0149 at 157.868 kb on + strand, within Atu0149 at 158.091 kb on + strand, within Atu0149 at 158.160 kb on - strand, within Atu0149 at 158.257 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Erythromycin 6.25 ug/mL remove 156,530 + Atu0147 0.87 +1.2 156,665 + -0.1 156,759 - Atu0148 0.13 -0.2 156,841 - Atu0148 0.27 +0.8 157,215 + Atu0148 0.90 -2.0 157,260 + +0.4 157,291 + -0.7 157,482 - -0.2 157,482 - -0.8 157,482 - -0.2 157,561 + Atu0149 0.12 -0.9 157,588 - Atu0149 0.16 -0.2 157,795 + Atu0149 0.41 +0.4 157,828 + Atu0149 0.45 -1.1 157,868 + Atu0149 0.50 -1.8 158,091 + Atu0149 0.78 -0.6 158,160 - Atu0149 0.87 +0.4 158,257 + +2.2
Or see this region's nucleotide sequence