Experiment: Bethi_Library_OD1.0
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu3482 and Atu3483 overlap by 4 nucleotides Atu3483 and Atu3484 are separated by 34 nucleotides Atu3484 and Atu3485 are separated by 50 nucleotides
Atu3482: Atu3482 - pantoate--beta-alanine ligase, at 531,140 to 532,018
Atu3482
Atu3483: Atu3483 - 3-methyl-2-oxobutanoate hydroxymethyltransferase, at 532,015 to 532,854
Atu3483
Atu3484: Atu3484 - hypothetical protein, at 532,889 to 534,181
Atu3484
Atu3485: Atu3485 - short chain dehydrogenase, at 534,232 to 536,328
Atu3485
Position (kb) 532
533
534
535 Strain fitness (log2 ratio) -2
-1
0
1 at 531.923 kb on - strand, within Atu3482 at 532.099 kb on - strand, within Atu3483 at 532.169 kb on + strand, within Atu3483 at 532.320 kb on + strand, within Atu3483 at 532.479 kb on - strand, within Atu3483 at 532.609 kb on - strand, within Atu3483 at 532.767 kb on + strand, within Atu3483 at 533.076 kb on + strand, within Atu3484 at 533.076 kb on + strand, within Atu3484 at 533.084 kb on - strand, within Atu3484 at 533.228 kb on - strand, within Atu3484 at 533.474 kb on + strand, within Atu3484 at 533.480 kb on + strand, within Atu3484 at 533.483 kb on - strand, within Atu3484 at 533.556 kb on + strand, within Atu3484 at 533.558 kb on + strand, within Atu3484 at 533.940 kb on - strand, within Atu3484 at 533.959 kb on + strand, within Atu3484 at 533.972 kb on - strand, within Atu3484 at 533.995 kb on + strand, within Atu3484 at 534.017 kb on + strand, within Atu3484 at 534.017 kb on - strand, within Atu3484 at 534.492 kb on + strand, within Atu3485 at 534.551 kb on + strand, within Atu3485 at 534.675 kb on + strand, within Atu3485 at 534.684 kb on - strand, within Atu3485 at 534.847 kb on - strand, within Atu3485 at 534.907 kb on + strand, within Atu3485 at 534.958 kb on + strand, within Atu3485
Per-strain Table
Position Strand Gene LocusTag Fraction Bethi_Library_OD1.0 remove 531,923 - Atu3482 0.89 +0.5 532,099 - Atu3483 0.10 +0.3 532,169 + Atu3483 0.18 +1.3 532,320 + Atu3483 0.36 +0.3 532,479 - Atu3483 0.55 -1.0 532,609 - Atu3483 0.71 -0.0 532,767 + Atu3483 0.90 -1.9 533,076 + Atu3484 0.14 -0.0 533,076 + Atu3484 0.14 +1.3 533,084 - Atu3484 0.15 +0.1 533,228 - Atu3484 0.26 +0.3 533,474 + Atu3484 0.45 +0.4 533,480 + Atu3484 0.46 +0.3 533,483 - Atu3484 0.46 +0.3 533,556 + Atu3484 0.52 +0.1 533,558 + Atu3484 0.52 +0.4 533,940 - Atu3484 0.81 -0.9 533,959 + Atu3484 0.83 +0.5 533,972 - Atu3484 0.84 -0.7 533,995 + Atu3484 0.86 -0.3 534,017 + Atu3484 0.87 +0.2 534,017 - Atu3484 0.87 -0.0 534,492 + Atu3485 0.12 +1.3 534,551 + Atu3485 0.15 -0.9 534,675 + Atu3485 0.21 -2.0 534,684 - Atu3485 0.22 -1.0 534,847 - Atu3485 0.29 -1.8 534,907 + Atu3485 0.32 +0.9 534,958 + Atu3485 0.35 -0.7
Or see this region's nucleotide sequence