Experiment: Bethi_Library_OD0.5
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu5424 and Atu5425 are separated by 10 nucleotides Atu5425 and Atu5426 are separated by 7 nucleotides Atu5426 and Atu5427 are separated by 1 nucleotides
Atu5424: Atu5424 - oxidoreductase, at 418,884 to 420,362
Atu5424
Atu5425: Atu5425 - 2-hydroxychromene-2-carboxylate isomerase, at 420,373 to 420,966
Atu5425
Atu5426: Atu5426 - hypothetical protein, at 420,974 to 421,480
Atu5426
Atu5427: Atu5427 - hypothetical protein, at 421,482 to 422,060
Atu5427
Position (kb)
420
421 Strain fitness (log2 ratio)
-1
0
1
2 at 419.459 kb on + strand, within Atu5424 at 419.474 kb on - strand, within Atu5424 at 419.501 kb on + strand, within Atu5424 at 419.587 kb on + strand, within Atu5424 at 419.589 kb on + strand, within Atu5424 at 419.606 kb on + strand, within Atu5424 at 419.671 kb on - strand, within Atu5424 at 419.773 kb on - strand, within Atu5424 at 419.793 kb on + strand, within Atu5424 at 419.801 kb on + strand, within Atu5424 at 419.803 kb on + strand, within Atu5424 at 419.997 kb on + strand, within Atu5424 at 420.020 kb on + strand, within Atu5424 at 420.020 kb on + strand, within Atu5424 at 420.020 kb on + strand, within Atu5424 at 420.020 kb on + strand, within Atu5424 at 420.020 kb on + strand, within Atu5424 at 420.020 kb on + strand, within Atu5424 at 420.028 kb on - strand, within Atu5424 at 420.032 kb on + strand, within Atu5424 at 420.032 kb on + strand, within Atu5424 at 420.050 kb on + strand, within Atu5424 at 420.129 kb on + strand, within Atu5424 at 420.220 kb on - strand at 420.221 kb on + strand at 420.266 kb on + strand at 420.274 kb on - strand at 420.277 kb on + strand at 420.318 kb on - strand at 420.318 kb on - strand at 420.318 kb on - strand at 420.331 kb on + strand at 420.347 kb on + strand at 420.365 kb on - strand at 420.371 kb on - strand at 420.423 kb on - strand at 420.427 kb on - strand at 420.449 kb on - strand, within Atu5425 at 420.475 kb on - strand, within Atu5425 at 420.535 kb on + strand, within Atu5425 at 420.549 kb on + strand, within Atu5425 at 420.675 kb on + strand, within Atu5425 at 420.683 kb on - strand, within Atu5425 at 420.686 kb on + strand, within Atu5425 at 420.706 kb on - strand, within Atu5425 at 420.730 kb on - strand, within Atu5425 at 420.743 kb on - strand, within Atu5425 at 420.799 kb on - strand, within Atu5425 at 420.799 kb on - strand, within Atu5425 at 420.816 kb on - strand, within Atu5425 at 420.841 kb on + strand, within Atu5425 at 420.852 kb on + strand, within Atu5425 at 420.919 kb on + strand at 420.922 kb on - strand at 420.927 kb on - strand at 420.986 kb on + strand at 421.025 kb on - strand, within Atu5426 at 421.029 kb on + strand, within Atu5426 at 421.074 kb on - strand, within Atu5426 at 421.118 kb on - strand, within Atu5426 at 421.143 kb on + strand, within Atu5426 at 421.143 kb on + strand, within Atu5426 at 421.201 kb on - strand, within Atu5426 at 421.222 kb on + strand, within Atu5426 at 421.222 kb on + strand, within Atu5426 at 421.366 kb on + strand, within Atu5426 at 421.382 kb on - strand, within Atu5426 at 421.438 kb on + strand at 421.581 kb on + strand, within Atu5427 at 421.607 kb on - strand, within Atu5427 at 421.610 kb on + strand, within Atu5427 at 421.663 kb on - strand, within Atu5427 at 421.757 kb on - strand, within Atu5427 at 421.787 kb on + strand, within Atu5427
Per-strain Table
Position Strand Gene LocusTag Fraction Bethi_Library_OD0.5 remove 419,459 + Atu5424 0.39 -0.4 419,474 - Atu5424 0.40 -0.7 419,501 + Atu5424 0.42 -0.0 419,587 + Atu5424 0.48 +0.5 419,589 + Atu5424 0.48 -0.5 419,606 + Atu5424 0.49 -0.1 419,671 - Atu5424 0.53 -0.0 419,773 - Atu5424 0.60 -0.4 419,793 + Atu5424 0.61 -0.2 419,801 + Atu5424 0.62 +0.5 419,803 + Atu5424 0.62 +0.3 419,997 + Atu5424 0.75 -0.3 420,020 + Atu5424 0.77 +0.2 420,020 + Atu5424 0.77 +0.1 420,020 + Atu5424 0.77 -0.9 420,020 + Atu5424 0.77 +0.1 420,020 + Atu5424 0.77 -0.1 420,020 + Atu5424 0.77 -0.2 420,028 - Atu5424 0.77 +0.3 420,032 + Atu5424 0.78 -0.5 420,032 + Atu5424 0.78 -0.3 420,050 + Atu5424 0.79 +0.0 420,129 + Atu5424 0.84 +0.1 420,220 - -0.2 420,221 + -0.4 420,266 + -0.1 420,274 - -0.2 420,277 + +1.3 420,318 - -0.3 420,318 - +0.2 420,318 - +1.9 420,331 + +0.7 420,347 + +0.9 420,365 - -0.0 420,371 - -0.4 420,423 - +0.2 420,427 - +0.0 420,449 - Atu5425 0.13 +0.6 420,475 - Atu5425 0.17 -0.2 420,535 + Atu5425 0.27 +0.2 420,549 + Atu5425 0.30 -1.2 420,675 + Atu5425 0.51 +1.4 420,683 - Atu5425 0.52 +0.6 420,686 + Atu5425 0.53 +1.0 420,706 - Atu5425 0.56 -0.3 420,730 - Atu5425 0.60 +0.4 420,743 - Atu5425 0.62 -0.0 420,799 - Atu5425 0.72 +0.9 420,799 - Atu5425 0.72 +0.2 420,816 - Atu5425 0.75 -0.0 420,841 + Atu5425 0.79 +0.2 420,852 + Atu5425 0.81 +1.3 420,919 + -0.2 420,922 - -0.8 420,927 - -1.1 420,986 + -0.1 421,025 - Atu5426 0.10 +0.3 421,029 + Atu5426 0.11 -1.0 421,074 - Atu5426 0.20 -0.7 421,118 - Atu5426 0.28 +1.3 421,143 + Atu5426 0.33 -0.2 421,143 + Atu5426 0.33 -0.7 421,201 - Atu5426 0.45 +0.4 421,222 + Atu5426 0.49 +0.2 421,222 + Atu5426 0.49 -0.4 421,366 + Atu5426 0.77 +0.7 421,382 - Atu5426 0.80 -0.7 421,438 + -0.1 421,581 + Atu5427 0.17 +0.1 421,607 - Atu5427 0.22 -0.3 421,610 + Atu5427 0.22 +1.3 421,663 - Atu5427 0.31 -0.7 421,757 - Atu5427 0.47 -1.0 421,787 + Atu5427 0.53 +1.5
Or see this region's nucleotide sequence