Experiment: Bethi_Library_OD0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu2547 and Atu2548 are separated by 586 nucleotides Atu2548 and Atu2549 are separated by 3 nucleotides Atu2549 and Atu2550 are separated by 199 nucleotides
Atu2547: Atu2547 - 16S ribosomal RNA, at 2,517,145 to 2,518,624
Atu2547
Atu2548: Atu2548 - phosphoribosylaminoimidazole-succinocarboxamide synthase, at 2,519,211 to 2,520,161
Atu2548
Atu2549: Atu2549 - transcriptional regulator, TetR family, at 2,520,165 to 2,520,824
Atu2549
Atu2550: Atu2550 - RND multidrug efflux membrane permease, at 2,521,024 to 2,522,226
Atu2550
Position (kb)
2519
2520
2521 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2519.116 kb on + strand at 2519.124 kb on - strand at 2519.124 kb on - strand at 2519.197 kb on + strand at 2519.246 kb on + strand at 2519.278 kb on + strand at 2519.286 kb on - strand at 2519.286 kb on - strand at 2519.288 kb on + strand at 2519.332 kb on + strand, within Atu2548 at 2519.337 kb on + strand, within Atu2548 at 2519.368 kb on + strand, within Atu2548 at 2519.401 kb on - strand, within Atu2548 at 2519.406 kb on - strand, within Atu2548 at 2519.408 kb on + strand, within Atu2548 at 2519.430 kb on + strand, within Atu2548 at 2519.486 kb on - strand, within Atu2548 at 2519.494 kb on + strand, within Atu2548 at 2519.525 kb on + strand, within Atu2548 at 2519.533 kb on + strand, within Atu2548 at 2519.547 kb on + strand, within Atu2548 at 2519.549 kb on + strand, within Atu2548 at 2519.559 kb on + strand, within Atu2548 at 2519.565 kb on + strand, within Atu2548 at 2519.570 kb on + strand, within Atu2548 at 2519.578 kb on - strand, within Atu2548 at 2519.608 kb on + strand, within Atu2548 at 2519.681 kb on - strand, within Atu2548 at 2519.692 kb on - strand, within Atu2548 at 2519.725 kb on + strand, within Atu2548 at 2519.733 kb on - strand, within Atu2548 at 2519.735 kb on + strand, within Atu2548 at 2519.748 kb on - strand, within Atu2548 at 2519.756 kb on + strand, within Atu2548 at 2519.756 kb on + strand, within Atu2548 at 2519.756 kb on + strand, within Atu2548 at 2519.756 kb on + strand, within Atu2548 at 2519.764 kb on - strand, within Atu2548 at 2519.764 kb on - strand, within Atu2548 at 2519.764 kb on - strand, within Atu2548 at 2519.774 kb on - strand, within Atu2548 at 2519.799 kb on - strand, within Atu2548 at 2519.799 kb on - strand, within Atu2548 at 2519.877 kb on - strand, within Atu2548 at 2519.963 kb on + strand, within Atu2548 at 2519.990 kb on + strand, within Atu2548 at 2520.038 kb on - strand, within Atu2548 at 2520.044 kb on + strand, within Atu2548 at 2520.044 kb on + strand, within Atu2548 at 2520.044 kb on + strand, within Atu2548 at 2520.052 kb on - strand, within Atu2548 at 2520.052 kb on - strand, within Atu2548 at 2520.098 kb on - strand at 2520.133 kb on + strand at 2520.133 kb on + strand at 2520.155 kb on + strand at 2520.155 kb on + strand at 2520.155 kb on + strand at 2520.163 kb on - strand at 2520.344 kb on - strand, within Atu2549 at 2520.347 kb on - strand, within Atu2549 at 2520.349 kb on - strand, within Atu2549 at 2520.434 kb on + strand, within Atu2549 at 2520.457 kb on + strand, within Atu2549 at 2520.475 kb on + strand, within Atu2549 at 2520.504 kb on + strand, within Atu2549 at 2520.512 kb on - strand, within Atu2549 at 2520.554 kb on - strand, within Atu2549 at 2520.560 kb on - strand, within Atu2549 at 2520.562 kb on + strand, within Atu2549 at 2520.593 kb on + strand, within Atu2549 at 2520.635 kb on + strand, within Atu2549 at 2520.640 kb on + strand, within Atu2549 at 2520.651 kb on + strand, within Atu2549 at 2520.707 kb on + strand, within Atu2549 at 2520.710 kb on - strand, within Atu2549 at 2520.711 kb on + strand, within Atu2549 at 2520.806 kb on - strand at 2520.866 kb on + strand at 2520.866 kb on + strand at 2520.874 kb on - strand at 2520.874 kb on - strand at 2520.881 kb on - strand at 2520.897 kb on + strand at 2520.926 kb on + strand at 2520.942 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bethi_Library_OD0.1 remove 2,519,116 + -0.3 2,519,124 - -0.4 2,519,124 - +0.2 2,519,197 + +0.1 2,519,246 + -1.1 2,519,278 + -0.7 2,519,286 - -1.4 2,519,286 - +1.2 2,519,288 + +0.2 2,519,332 + Atu2548 0.13 -0.6 2,519,337 + Atu2548 0.13 -0.7 2,519,368 + Atu2548 0.17 -1.1 2,519,401 - Atu2548 0.20 -1.6 2,519,406 - Atu2548 0.21 +0.2 2,519,408 + Atu2548 0.21 -1.6 2,519,430 + Atu2548 0.23 -0.4 2,519,486 - Atu2548 0.29 -1.4 2,519,494 + Atu2548 0.30 -0.5 2,519,525 + Atu2548 0.33 +2.2 2,519,533 + Atu2548 0.34 -0.8 2,519,547 + Atu2548 0.35 -0.1 2,519,549 + Atu2548 0.36 -1.2 2,519,559 + Atu2548 0.37 -0.4 2,519,565 + Atu2548 0.37 -0.1 2,519,570 + Atu2548 0.38 -0.9 2,519,578 - Atu2548 0.39 -1.6 2,519,608 + Atu2548 0.42 -0.4 2,519,681 - Atu2548 0.49 -0.7 2,519,692 - Atu2548 0.51 -0.6 2,519,725 + Atu2548 0.54 -0.7 2,519,733 - Atu2548 0.55 -0.4 2,519,735 + Atu2548 0.55 -1.4 2,519,748 - Atu2548 0.56 -0.6 2,519,756 + Atu2548 0.57 +0.4 2,519,756 + Atu2548 0.57 +0.0 2,519,756 + Atu2548 0.57 -0.8 2,519,756 + Atu2548 0.57 -1.1 2,519,764 - Atu2548 0.58 -0.6 2,519,764 - Atu2548 0.58 -0.2 2,519,764 - Atu2548 0.58 -1.1 2,519,774 - Atu2548 0.59 -0.3 2,519,799 - Atu2548 0.62 -0.9 2,519,799 - Atu2548 0.62 -2.1 2,519,877 - Atu2548 0.70 +0.2 2,519,963 + Atu2548 0.79 -0.4 2,519,990 + Atu2548 0.82 +1.2 2,520,038 - Atu2548 0.87 +0.2 2,520,044 + Atu2548 0.88 -0.2 2,520,044 + Atu2548 0.88 +0.3 2,520,044 + Atu2548 0.88 -0.8 2,520,052 - Atu2548 0.88 -3.2 2,520,052 - Atu2548 0.88 -0.8 2,520,098 - +0.2 2,520,133 + -0.8 2,520,133 + +0.1 2,520,155 + -1.6 2,520,155 + -0.4 2,520,155 + +0.1 2,520,163 - -0.4 2,520,344 - Atu2549 0.27 -2.1 2,520,347 - Atu2549 0.28 +0.6 2,520,349 - Atu2549 0.28 -0.1 2,520,434 + Atu2549 0.41 -0.4 2,520,457 + Atu2549 0.44 +0.5 2,520,475 + Atu2549 0.47 -0.6 2,520,504 + Atu2549 0.51 -0.2 2,520,512 - Atu2549 0.53 -0.3 2,520,554 - Atu2549 0.59 -0.6 2,520,560 - Atu2549 0.60 +0.1 2,520,562 + Atu2549 0.60 -1.6 2,520,593 + Atu2549 0.65 -0.9 2,520,635 + Atu2549 0.71 -0.7 2,520,640 + Atu2549 0.72 +1.2 2,520,651 + Atu2549 0.74 -0.2 2,520,707 + Atu2549 0.82 -0.2 2,520,710 - Atu2549 0.83 -0.4 2,520,711 + Atu2549 0.83 +2.2 2,520,806 - -0.7 2,520,866 + -0.5 2,520,866 + -0.3 2,520,874 - -0.0 2,520,874 - +1.2 2,520,881 - +0.1 2,520,897 + +0.5 2,520,926 + -0.6 2,520,942 - +0.2
Or see this region's nucleotide sequence