Experiment: Bethi_Library_OD0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu1528 and Atu1529 overlap by 4 nucleotides Atu1529 and Atu1530 overlap by 4 nucleotides Atu1530 and Atu1531 are separated by 120 nucleotides Atu1531 and Atu1532 are separated by 11 nucleotides Atu1532 and Atu1533 are separated by 16 nucleotides
Atu1528: Atu1528 - nitrogen fixation protein FixI, at 1,519,351 to 1,521,642
Atu1528
Atu1529: Atu1529 - nitrogen fixation protein FixH, at 1,521,639 to 1,522,139
Atu1529
Atu1530: Atu1530 - nitrogen fixation protein FixG, at 1,522,136 to 1,523,719
Atu1530
Atu1531: Atu1531 - hypothetical protein, at 1,523,840 to 1,524,244
Atu1531
Atu1532: Atu1532 - glyoxalase/bleomycin resistance protein/dioxygenase superfamily protein, at 1,524,256 to 1,524,612
Atu1532
Atu1533: Atu1533 - glyoxalase/bleomycin resistance protein/dioxygenase superfamily protein, at 1,524,629 to 1,525,075
Atu1533
Position (kb)
1522
1523
1524 Strain fitness (log2 ratio)
-2
-1
0
1 at 1521.136 kb on - strand, within Atu1528 at 1521.167 kb on + strand, within Atu1528 at 1521.216 kb on - strand, within Atu1528 at 1521.216 kb on - strand, within Atu1528 at 1521.266 kb on - strand, within Atu1528 at 1521.268 kb on + strand, within Atu1528 at 1521.271 kb on - strand, within Atu1528 at 1521.380 kb on + strand, within Atu1528 at 1521.470 kb on + strand at 1521.640 kb on + strand at 1521.698 kb on + strand, within Atu1529 at 1521.939 kb on + strand, within Atu1529 at 1521.966 kb on - strand, within Atu1529 at 1522.029 kb on + strand, within Atu1529 at 1522.065 kb on + strand, within Atu1529 at 1522.291 kb on + strand at 1522.305 kb on - strand, within Atu1530 at 1522.476 kb on - strand, within Atu1530 at 1522.493 kb on - strand, within Atu1530 at 1522.511 kb on + strand, within Atu1530 at 1522.518 kb on + strand, within Atu1530 at 1522.557 kb on + strand, within Atu1530 at 1522.557 kb on + strand, within Atu1530 at 1522.557 kb on + strand, within Atu1530 at 1522.781 kb on - strand, within Atu1530 at 1522.783 kb on + strand, within Atu1530 at 1522.791 kb on - strand, within Atu1530 at 1522.831 kb on + strand, within Atu1530 at 1522.839 kb on - strand, within Atu1530 at 1523.010 kb on + strand, within Atu1530 at 1523.011 kb on - strand, within Atu1530 at 1523.243 kb on - strand, within Atu1530 at 1523.289 kb on + strand, within Atu1530 at 1523.468 kb on + strand, within Atu1530 at 1523.476 kb on - strand, within Atu1530 at 1523.627 kb on - strand at 1523.655 kb on + strand at 1523.889 kb on + strand, within Atu1531 at 1523.966 kb on - strand, within Atu1531 at 1524.048 kb on + strand, within Atu1531 at 1524.085 kb on + strand, within Atu1531 at 1524.128 kb on + strand, within Atu1531 at 1524.135 kb on + strand, within Atu1531 at 1524.136 kb on - strand, within Atu1531 at 1524.143 kb on - strand, within Atu1531 at 1524.160 kb on + strand, within Atu1531 at 1524.581 kb on + strand at 1524.589 kb on - strand at 1524.589 kb on - strand at 1524.589 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bethi_Library_OD0.1 remove 1,521,136 - Atu1528 0.78 +0.9 1,521,167 + Atu1528 0.79 -1.4 1,521,216 - Atu1528 0.81 -2.0 1,521,216 - Atu1528 0.81 +0.4 1,521,266 - Atu1528 0.84 -0.3 1,521,268 + Atu1528 0.84 -0.2 1,521,271 - Atu1528 0.84 -0.6 1,521,380 + Atu1528 0.89 +0.0 1,521,470 + -2.2 1,521,640 + +0.2 1,521,698 + Atu1529 0.12 -0.2 1,521,939 + Atu1529 0.60 -0.3 1,521,966 - Atu1529 0.65 +0.2 1,522,029 + Atu1529 0.78 -0.4 1,522,065 + Atu1529 0.85 -0.1 1,522,291 + -1.2 1,522,305 - Atu1530 0.11 -1.6 1,522,476 - Atu1530 0.21 -0.8 1,522,493 - Atu1530 0.23 -0.5 1,522,511 + Atu1530 0.24 -0.6 1,522,518 + Atu1530 0.24 -0.4 1,522,557 + Atu1530 0.27 -1.0 1,522,557 + Atu1530 0.27 -0.4 1,522,557 + Atu1530 0.27 -0.8 1,522,781 - Atu1530 0.41 -1.2 1,522,783 + Atu1530 0.41 -0.6 1,522,791 - Atu1530 0.41 +0.2 1,522,831 + Atu1530 0.44 -0.8 1,522,839 - Atu1530 0.44 +1.2 1,523,010 + Atu1530 0.55 -2.1 1,523,011 - Atu1530 0.55 -0.7 1,523,243 - Atu1530 0.70 -0.5 1,523,289 + Atu1530 0.73 -1.7 1,523,468 + Atu1530 0.84 +1.2 1,523,476 - Atu1530 0.85 +0.2 1,523,627 - -0.2 1,523,655 + -0.5 1,523,889 + Atu1531 0.12 -0.2 1,523,966 - Atu1531 0.31 -0.1 1,524,048 + Atu1531 0.51 +1.5 1,524,085 + Atu1531 0.60 -0.8 1,524,128 + Atu1531 0.71 -0.3 1,524,135 + Atu1531 0.73 +0.2 1,524,136 - Atu1531 0.73 -0.6 1,524,143 - Atu1531 0.75 +0.5 1,524,160 + Atu1531 0.79 +0.6 1,524,581 + -0.2 1,524,589 - +1.5 1,524,589 - +0.4 1,524,589 - +0.7
Or see this region's nucleotide sequence