Experiment: Bethi_Library_OD0.05
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu2660 and Atu2661 are separated by 5 nucleotides Atu2661 and Atu2662 are separated by 169 nucleotides Atu2662 and Atu2663 are separated by 215 nucleotides
Atu2660: Atu2660 - hypothetical protein, at 2,643,260 to 2,643,811
Atu2660
Atu2661: Atu2661 - inorganic pyrophosphatase, at 2,643,817 to 2,644,350
Atu2661
Atu2662: Atu2662 - acetyltransferase, at 2,644,520 to 2,645,026
Atu2662
Atu2663: Atu2663 - GTP-binding tyrosin phosphorylated protein, at 2,645,242 to 2,647,062
Atu2663
Position (kb)
2644
2645
2646 Strain fitness (log2 ratio)
-1
0
1 at 2643.543 kb on + strand, within Atu2660 at 2643.666 kb on - strand, within Atu2660 at 2643.716 kb on + strand, within Atu2660 at 2643.823 kb on - strand at 2644.454 kb on - strand at 2644.506 kb on + strand at 2644.513 kb on + strand at 2644.521 kb on - strand at 2644.521 kb on - strand at 2644.521 kb on - strand at 2644.631 kb on + strand, within Atu2662 at 2644.871 kb on - strand, within Atu2662 at 2645.098 kb on + strand at 2645.132 kb on + strand at 2645.208 kb on - strand at 2645.235 kb on + strand at 2645.262 kb on + strand at 2645.601 kb on + strand, within Atu2663 at 2645.690 kb on + strand, within Atu2663
Per-strain Table
Position Strand Gene LocusTag Fraction Bethi_Library_OD0.05 remove 2,643,543 + Atu2660 0.51 +0.2 2,643,666 - Atu2660 0.74 +0.5 2,643,716 + Atu2660 0.83 -0.4 2,643,823 - +0.7 2,644,454 - -0.2 2,644,506 + +0.8 2,644,513 + -0.1 2,644,521 - -0.6 2,644,521 - +0.0 2,644,521 - -0.4 2,644,631 + Atu2662 0.22 -0.3 2,644,871 - Atu2662 0.69 +1.4 2,645,098 + +0.9 2,645,132 + -1.4 2,645,208 - -0.7 2,645,235 + -0.5 2,645,262 + +1.4 2,645,601 + Atu2663 0.20 +1.0 2,645,690 + Atu2663 0.25 -0.6
Or see this region's nucleotide sequence