Experiment: Bethi_Library_OD0.05
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu8174 and Atu2220 overlap by 11 nucleotides Atu2220 and Atu2221 overlap by 8 nucleotides Atu2221 and Atu2222 are separated by 20 nucleotides
Atu8174: Atu8174 - hypothetical protein, at 2,187,518 to 2,188,363
Atu8174
Atu2220: Atu2220 - hypothetical protein, at 2,188,353 to 2,189,843
Atu2220
Atu2221: Atu2221 - Lipid A biosynthesis protein, at 2,189,836 to 2,190,123
Atu2221
Atu2222: Atu2222 - dolichol-phosphate mannosyltransferase, at 2,190,144 to 2,190,983
Atu2222
Position (kb)
2188
2189
2190 Strain fitness (log2 ratio)
-1
0
1
2 at 2187.375 kb on - strand at 2187.375 kb on - strand at 2187.380 kb on + strand at 2187.388 kb on - strand at 2187.435 kb on + strand at 2187.504 kb on + strand at 2187.696 kb on + strand, within Atu8174 at 2187.699 kb on - strand, within Atu8174 at 2188.166 kb on + strand, within Atu8174 at 2188.229 kb on - strand, within Atu8174 at 2188.229 kb on - strand, within Atu8174 at 2188.277 kb on + strand, within Atu8174 at 2188.312 kb on + strand at 2188.441 kb on + strand at 2188.593 kb on - strand, within Atu2220 at 2188.757 kb on + strand, within Atu2220 at 2188.758 kb on + strand, within Atu2220 at 2188.808 kb on - strand, within Atu2220 at 2188.913 kb on - strand, within Atu2220 at 2189.130 kb on - strand, within Atu2220 at 2189.176 kb on - strand, within Atu2220 at 2189.335 kb on + strand, within Atu2220 at 2189.358 kb on - strand, within Atu2220 at 2189.436 kb on + strand, within Atu2220 at 2189.456 kb on - strand, within Atu2220 at 2189.592 kb on + strand, within Atu2220 at 2189.640 kb on - strand, within Atu2220 at 2189.648 kb on + strand, within Atu2220 at 2189.735 kb on + strand at 2189.753 kb on - strand at 2189.828 kb on - strand at 2189.861 kb on - strand at 2189.895 kb on + strand, within Atu2221 at 2189.895 kb on + strand, within Atu2221 at 2189.940 kb on + strand, within Atu2221 at 2189.948 kb on - strand, within Atu2221 at 2190.079 kb on + strand, within Atu2221 at 2190.087 kb on - strand, within Atu2221 at 2190.205 kb on + strand at 2190.346 kb on + strand, within Atu2222 at 2190.367 kb on + strand, within Atu2222 at 2190.367 kb on + strand, within Atu2222 at 2190.496 kb on + strand, within Atu2222 at 2190.632 kb on - strand, within Atu2222 at 2190.652 kb on + strand, within Atu2222 at 2190.660 kb on + strand, within Atu2222 at 2190.822 kb on + strand, within Atu2222 at 2190.833 kb on + strand, within Atu2222
Per-strain Table
Position Strand Gene LocusTag Fraction Bethi_Library_OD0.05 remove 2,187,375 - +0.3 2,187,375 - -0.5 2,187,380 + -1.6 2,187,388 - -1.1 2,187,435 + -0.5 2,187,504 + +0.6 2,187,696 + Atu8174 0.21 -0.2 2,187,699 - Atu8174 0.21 +0.7 2,188,166 + Atu8174 0.77 +0.0 2,188,229 - Atu8174 0.84 -0.2 2,188,229 - Atu8174 0.84 -0.4 2,188,277 + Atu8174 0.90 -0.7 2,188,312 + +1.3 2,188,441 + +0.1 2,188,593 - Atu2220 0.16 +1.2 2,188,757 + Atu2220 0.27 +0.4 2,188,758 + Atu2220 0.27 +0.2 2,188,808 - Atu2220 0.31 +1.3 2,188,913 - Atu2220 0.38 +0.6 2,189,130 - Atu2220 0.52 +0.5 2,189,176 - Atu2220 0.55 +1.6 2,189,335 + Atu2220 0.66 +0.9 2,189,358 - Atu2220 0.67 +0.2 2,189,436 + Atu2220 0.73 -0.1 2,189,456 - Atu2220 0.74 -0.1 2,189,592 + Atu2220 0.83 +0.0 2,189,640 - Atu2220 0.86 -0.2 2,189,648 + Atu2220 0.87 +1.5 2,189,735 + +0.0 2,189,753 - +0.8 2,189,828 - -1.2 2,189,861 - -0.2 2,189,895 + Atu2221 0.20 -0.7 2,189,895 + Atu2221 0.20 +1.5 2,189,940 + Atu2221 0.36 +0.6 2,189,948 - Atu2221 0.39 +0.1 2,190,079 + Atu2221 0.84 +2.6 2,190,087 - Atu2221 0.87 +0.2 2,190,205 + +0.1 2,190,346 + Atu2222 0.24 -0.1 2,190,367 + Atu2222 0.27 +0.1 2,190,367 + Atu2222 0.27 -0.6 2,190,496 + Atu2222 0.42 -0.8 2,190,632 - Atu2222 0.58 +0.1 2,190,652 + Atu2222 0.60 -0.7 2,190,660 + Atu2222 0.61 +0.1 2,190,822 + Atu2222 0.81 +0.5 2,190,833 + Atu2222 0.82 -0.5
Or see this region's nucleotide sequence