Experiment: Bethi_Library_OD0.01
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu1100 and Atu1101 are separated by 5 nucleotides Atu1101 and Atu1102 are separated by 1 nucleotides Atu1102 and Atu1103 are separated by 71 nucleotides Atu1103 and Atu1104 overlap by 1 nucleotides
Atu1100: Atu1100 - hypothetical protein, at 1,089,547 to 1,090,434
Atu1100
Atu1101: Atu1101 - guanylate kinase, at 1,090,440 to 1,091,102
Atu1101
Atu1102: Atu1102 - hypothetical protein, at 1,091,104 to 1,091,727
Atu1102
Atu1103: Atu1103 - rRNA-adenine N6,N6-dimethyltransferase, at 1,091,799 to 1,092,629
Atu1103
Atu1104: Atu1104 - pyridoxal phosphate biosynthesis protein, at 1,092,629 to 1,093,651
Atu1104
Position (kb)
1091
1092 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1090.238 kb on + strand, within Atu1100 at 1090.251 kb on - strand, within Atu1100 at 1090.256 kb on - strand, within Atu1100 at 1090.269 kb on - strand, within Atu1100 at 1091.178 kb on - strand, within Atu1102 at 1091.368 kb on - strand, within Atu1102 at 1091.421 kb on - strand, within Atu1102 at 1091.457 kb on + strand, within Atu1102 at 1091.481 kb on + strand, within Atu1102 at 1091.587 kb on + strand, within Atu1102 at 1091.742 kb on + strand at 1091.742 kb on + strand at 1091.814 kb on - strand at 1091.959 kb on - strand, within Atu1103 at 1091.988 kb on + strand, within Atu1103 at 1092.357 kb on - strand, within Atu1103 at 1092.659 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bethi_Library_OD0.01 remove 1,090,238 + Atu1100 0.78 -0.4 1,090,251 - Atu1100 0.79 +1.5 1,090,256 - Atu1100 0.80 -0.1 1,090,269 - Atu1100 0.81 -0.1 1,091,178 - Atu1102 0.12 -0.4 1,091,368 - Atu1102 0.42 -1.8 1,091,421 - Atu1102 0.51 +0.2 1,091,457 + Atu1102 0.57 -0.4 1,091,481 + Atu1102 0.60 -1.9 1,091,587 + Atu1102 0.77 -0.9 1,091,742 + +0.2 1,091,742 + +0.4 1,091,814 - -0.5 1,091,959 - Atu1103 0.19 -0.4 1,091,988 + Atu1103 0.23 +2.5 1,092,357 - Atu1103 0.67 +0.2 1,092,659 - -0.2
Or see this region's nucleotide sequence