Strain Fitness in Pseudomonas putida KT2440 around PP_2269

Experiment: Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=144_hrs

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPP_2267 and PP_2268 are separated by 22 nucleotidesPP_2268 and PP_2269 are separated by 2 nucleotidesPP_2269 and PP_2270 are separated by 271 nucleotides PP_2267: PP_2267 - putative Phage single-stranded DNA-binding protein, at 2,589,954 to 2,590,634 _2267 PP_2268: PP_2268 - Phage endodeoxyribonuclease I, at 2,590,657 to 2,591,082 _2268 PP_2269: PP_2269 - putative N-acetylmuramoyl-L-alanine amidase, at 2,591,085 to 2,591,534 _2269 PP_2270: PP_2270 - putative DNA primase/helicase protein, at 2,591,806 to 2,593,257 _2270 Position (kb) 2591 2592Strain fitness (log2 ratio) -2 -1 0 1 2at 2590.184 kb on - strand, within PP_2267at 2590.184 kb on - strand, within PP_2267at 2590.407 kb on + strand, within PP_2267at 2590.408 kb on - strand, within PP_2267at 2590.408 kb on - strand, within PP_2267at 2590.408 kb on - strand, within PP_2267at 2590.618 kb on - strandat 2590.669 kb on + strandat 2590.759 kb on + strand, within PP_2268at 2591.080 kb on + strandat 2591.128 kb on - strandat 2591.296 kb on + strand, within PP_2269at 2591.297 kb on - strand, within PP_2269at 2591.297 kb on - strand, within PP_2269at 2591.306 kb on + strand, within PP_2269at 2591.307 kb on - strand, within PP_2269at 2591.307 kb on - strand, within PP_2269at 2591.508 kb on - strandat 2591.531 kb on - strandat 2591.912 kb on + strandat 2591.913 kb on - strandat 2592.087 kb on + strand, within PP_2270at 2592.087 kb on + strand, within PP_2270at 2592.087 kb on + strand, within PP_2270at 2592.088 kb on - strand, within PP_2270at 2592.088 kb on - strand, within PP_2270at 2592.266 kb on + strand, within PP_2270at 2592.266 kb on + strand, within PP_2270at 2592.267 kb on - strand, within PP_2270at 2592.299 kb on + strand, within PP_2270at 2592.379 kb on + strand, within PP_2270at 2592.395 kb on + strand, within PP_2270at 2592.395 kb on + strand, within PP_2270at 2592.396 kb on - strand, within PP_2270at 2592.396 kb on - strand, within PP_2270at 2592.396 kb on - strand, within PP_2270at 2592.497 kb on - strand, within PP_2270at 2592.525 kb on + strand, within PP_2270at 2592.525 kb on + strand, within PP_2270at 2592.525 kb on + strand, within PP_2270at 2592.526 kb on - strand, within PP_2270

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Per-strain Table

Position Strand Gene LocusTag Fraction Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=144_hrs
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2,590,184 - PP_2267 0.34 +0.7
2,590,184 - PP_2267 0.34 -0.6
2,590,407 + PP_2267 0.67 +0.1
2,590,408 - PP_2267 0.67 +0.1
2,590,408 - PP_2267 0.67 -0.5
2,590,408 - PP_2267 0.67 -0.4
2,590,618 - +0.1
2,590,669 + +1.0
2,590,759 + PP_2268 0.24 -0.7
2,591,080 + -1.9
2,591,128 - +0.2
2,591,296 + PP_2269 0.47 +0.5
2,591,297 - PP_2269 0.47 +0.9
2,591,297 - PP_2269 0.47 +0.4
2,591,306 + PP_2269 0.49 +0.5
2,591,307 - PP_2269 0.49 +0.5
2,591,307 - PP_2269 0.49 +2.4
2,591,508 - +1.0
2,591,531 - -0.8
2,591,912 + -0.2
2,591,913 - -0.8
2,592,087 + PP_2270 0.19 +0.3
2,592,087 + PP_2270 0.19 +1.2
2,592,087 + PP_2270 0.19 +1.0
2,592,088 - PP_2270 0.19 +0.1
2,592,088 - PP_2270 0.19 +0.1
2,592,266 + PP_2270 0.32 +1.3
2,592,266 + PP_2270 0.32 -0.1
2,592,267 - PP_2270 0.32 -0.7
2,592,299 + PP_2270 0.34 +0.1
2,592,379 + PP_2270 0.39 -0.1
2,592,395 + PP_2270 0.41 -0.8
2,592,395 + PP_2270 0.41 -1.1
2,592,396 - PP_2270 0.41 +0.2
2,592,396 - PP_2270 0.41 -1.2
2,592,396 - PP_2270 0.41 +0.5
2,592,497 - PP_2270 0.48 -0.6
2,592,525 + PP_2270 0.50 +1.0
2,592,525 + PP_2270 0.50 -0.5
2,592,525 + PP_2270 0.50 -0.2
2,592,526 - PP_2270 0.50 -0.6

Or see this region's nucleotide sequence