Strain Fitness in Pseudomonas putida KT2440 around PP_1767

Experiment: Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=144_hrs

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPP_1766 and PP_1767 are separated by 365 nucleotidesPP_1767 and PP_1768 are separated by 147 nucleotides PP_1766: PP_1766 - methylthioribose-1-phosphate isomerase, at 1,969,630 to 1,970,706 _1766 PP_1767: PP_1767 - DNA gyrase, subunit A, at 1,971,072 to 1,973,843 _1767 PP_1768: PP_1768 - phosphoserine aminotransferase, at 1,973,991 to 1,975,076 _1768 Position (kb) 1971 1972 1973 1974Strain fitness (log2 ratio) -3 -2 -1 0 1at 1970.215 kb on + strand, within PP_1766at 1970.410 kb on + strand, within PP_1766at 1970.410 kb on + strand, within PP_1766at 1970.410 kb on + strand, within PP_1766at 1970.410 kb on + strand, within PP_1766at 1970.411 kb on - strand, within PP_1766at 1970.600 kb on + strandat 1970.898 kb on + strandat 1973.785 kb on + strandat 1973.841 kb on + strandat 1973.841 kb on + strandat 1973.841 kb on + strandat 1973.841 kb on + strandat 1973.841 kb on + strandat 1973.841 kb on + strandat 1973.845 kb on + strandat 1973.851 kb on + strandat 1973.893 kb on + strandat 1973.894 kb on - strandat 1973.955 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=144_hrs
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1,970,215 + PP_1766 0.54 -0.2
1,970,410 + PP_1766 0.72 -0.3
1,970,410 + PP_1766 0.72 +0.9
1,970,410 + PP_1766 0.72 -0.3
1,970,410 + PP_1766 0.72 -0.5
1,970,411 - PP_1766 0.73 -3.6
1,970,600 + +1.5
1,970,898 + +0.1
1,973,785 + -2.1
1,973,841 + -2.9
1,973,841 + -0.3
1,973,841 + -1.5
1,973,841 + -1.4
1,973,841 + -2.6
1,973,841 + -1.6
1,973,845 + -2.5
1,973,851 + +0.1
1,973,893 + -2.0
1,973,894 - +0.5
1,973,955 + -1.4

Or see this region's nucleotide sequence