Strain Fitness in Pseudomonas putida KT2440 around PP_4808

Experiment: Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=144_hrs

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPP_4807 and PP_4808 are separated by 16 nucleotidesPP_4808 and PP_4809 overlap by 41 nucleotidesPP_4809 and PP_4810 are separated by 43 nucleotides PP_4807: PP_4807 - transpeptidase (penicillin-binding protein 2), at 5,470,015 to 5,471,904 _4807 PP_4808: PP_4808 - 23S rRNA m3psi1915 methyltransferase, at 5,471,921 to 5,472,439 _4808 PP_4809: PP_4809 - Ribosomal silencing factor RsfS, at 5,472,399 to 5,472,818 _4809 PP_4810: PP_4810 - Probable nicotinate-nucleotide adenylyltransferase, at 5,472,862 to 5,473,554 _4810 Position (kb) 5471 5472 5473Strain fitness (log2 ratio) -2 -1 0 1 2at 5471.915 kb on - strandat 5471.915 kb on - strandat 5471.932 kb on - strandat 5471.965 kb on - strandat 5471.991 kb on - strand, within PP_4808at 5472.322 kb on - strand, within PP_4808at 5472.322 kb on - strand, within PP_4808at 5472.401 kb on - strandat 5472.401 kb on - strandat 5472.697 kb on + strand, within PP_4809at 5472.698 kb on - strand, within PP_4809at 5472.698 kb on - strand, within PP_4809at 5472.821 kb on - strandat 5472.821 kb on - strandat 5472.821 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=144_hrs
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5,471,915 - +0.2
5,471,915 - -0.6
5,471,932 - -2.1
5,471,965 - +0.4
5,471,991 - PP_4808 0.13 +0.3
5,472,322 - PP_4808 0.77 +1.2
5,472,322 - PP_4808 0.77 +2.5
5,472,401 - +1.0
5,472,401 - +0.4
5,472,697 + PP_4809 0.71 +1.1
5,472,698 - PP_4809 0.71 +1.8
5,472,698 - PP_4809 0.71 -0.3
5,472,821 - -0.6
5,472,821 - +0.1
5,472,821 - -2.1

Or see this region's nucleotide sequence