Strain Fitness in Pseudomonas putida KT2440 around PP_3116

Experiment: Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=144_hrs

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPP_3114 and PP_3115 are separated by 14 nucleotidesPP_3115 and PP_3116 are separated by 405 nucleotidesPP_3116 and PP_3117 overlap by 1 nucleotidesPP_3117 and PP_3118 are separated by 6 nucleotides PP_3114: PP_3114 - transposase, at 3,523,288 to 3,524,820 _3114 PP_3115: PP_3115 - transposase, at 3,524,835 to 3,525,149 _3115 PP_3116: PP_3116 - transcriptional repressor, at 3,525,555 to 3,526,163 _3116 PP_3117: PP_3117 - putative protein involved in error-prone processing of DNA lesions, at 3,526,163 to 3,526,783 _3117 PP_3118: PP_3118 - DNA linked enzyme involved in DNA repair, at 3,526,790 to 3,528,208 _3118 Position (kb) 3525 3526 3527Strain fitness (log2 ratio) -2 -1 0 1 2at 3525.192 kb on + strandat 3525.192 kb on + strandat 3525.192 kb on + strandat 3525.192 kb on + strandat 3525.422 kb on - strandat 3525.493 kb on - strandat 3525.493 kb on - strandat 3525.493 kb on - strandat 3525.566 kb on + strandat 3525.566 kb on + strandat 3525.567 kb on - strandat 3525.718 kb on - strand, within PP_3116at 3525.751 kb on + strand, within PP_3116at 3525.751 kb on + strand, within PP_3116at 3525.793 kb on + strand, within PP_3116at 3526.086 kb on + strand, within PP_3116at 3526.086 kb on + strand, within PP_3116at 3526.479 kb on + strand, within PP_3117at 3526.479 kb on + strand, within PP_3117at 3526.605 kb on + strand, within PP_3117at 3526.605 kb on + strand, within PP_3117at 3526.761 kb on + strandat 3526.762 kb on - strandat 3527.055 kb on - strand, within PP_3118at 3527.085 kb on - strand, within PP_3118at 3527.085 kb on - strand, within PP_3118

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Per-strain Table

Position Strand Gene LocusTag Fraction Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=144_hrs
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3,525,192 + +1.1
3,525,192 + +1.6
3,525,192 + -0.8
3,525,192 + -0.2
3,525,422 - -2.1
3,525,493 - -1.3
3,525,493 - +2.0
3,525,493 - +0.5
3,525,566 + -1.5
3,525,566 + +0.2
3,525,567 - +1.3
3,525,718 - PP_3116 0.27 +1.6
3,525,751 + PP_3116 0.32 +1.7
3,525,751 + PP_3116 0.32 +0.2
3,525,793 + PP_3116 0.39 -0.2
3,526,086 + PP_3116 0.87 +1.4
3,526,086 + PP_3116 0.87 +1.7
3,526,479 + PP_3117 0.51 +0.3
3,526,479 + PP_3117 0.51 -0.8
3,526,605 + PP_3117 0.71 -0.4
3,526,605 + PP_3117 0.71 -2.2
3,526,761 + -2.0
3,526,762 - -1.7
3,527,055 - PP_3118 0.19 +0.9
3,527,085 - PP_3118 0.21 -0.8
3,527,085 - PP_3118 0.21 -1.4

Or see this region's nucleotide sequence