Strain Fitness in Pseudomonas putida KT2440 around PP_2800

Experiment: Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=144_hrs

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPP_2799 and PP_2800 are separated by 95 nucleotidesPP_2800 and PP_2801 are separated by 37 nucleotides PP_2799: PP_2799 - Aminotransferase, class III, at 3,189,270 to 3,190,649 _2799 PP_2800: PP_2800 - putative Diaminobutyrate-2-oxoglutarate transaminase, at 3,190,745 to 3,191,998 _2800 PP_2801: PP_2801 - gamma-aminobutyraldehyde dehydrogenase, at 3,192,036 to 3,193,460 _2801 Position (kb) 3190 3191 3192Strain fitness (log2 ratio) -3 -2 -1 0 1at 3189.775 kb on + strand, within PP_2799at 3189.776 kb on - strand, within PP_2799at 3189.776 kb on - strand, within PP_2799at 3189.827 kb on - strand, within PP_2799at 3189.934 kb on + strand, within PP_2799at 3190.023 kb on - strand, within PP_2799at 3190.023 kb on - strand, within PP_2799at 3190.024 kb on + strand, within PP_2799at 3190.024 kb on + strand, within PP_2799at 3190.024 kb on + strand, within PP_2799at 3190.025 kb on - strand, within PP_2799at 3190.025 kb on - strand, within PP_2799at 3190.025 kb on - strand, within PP_2799at 3190.056 kb on - strand, within PP_2799at 3190.056 kb on - strand, within PP_2799at 3190.201 kb on + strand, within PP_2799at 3190.405 kb on + strand, within PP_2799at 3190.405 kb on + strand, within PP_2799at 3190.408 kb on + strand, within PP_2799at 3191.478 kb on + strand, within PP_2800at 3191.494 kb on - strand, within PP_2800at 3191.583 kb on + strand, within PP_2800at 3191.592 kb on + strand, within PP_2800at 3191.691 kb on + strand, within PP_2800at 3191.841 kb on + strand, within PP_2800at 3191.841 kb on + strand, within PP_2800at 3191.842 kb on - strand, within PP_2800at 3191.878 kb on - strandat 3192.003 kb on - strandat 3192.070 kb on + strandat 3192.085 kb on + strandat 3192.086 kb on - strandat 3192.086 kb on - strandat 3192.429 kb on + strand, within PP_2801at 3192.429 kb on + strand, within PP_2801at 3192.429 kb on + strand, within PP_2801at 3192.430 kb on - strand, within PP_2801at 3192.430 kb on - strand, within PP_2801at 3192.430 kb on - strand, within PP_2801at 3192.430 kb on - strand, within PP_2801at 3192.431 kb on + strand, within PP_2801at 3192.431 kb on + strand, within PP_2801at 3192.601 kb on + strand, within PP_2801at 3192.602 kb on - strand, within PP_2801at 3192.602 kb on - strand, within PP_2801at 3192.641 kb on - strand, within PP_2801at 3192.728 kb on + strand, within PP_2801at 3192.728 kb on + strand, within PP_2801at 3192.729 kb on - strand, within PP_2801at 3192.741 kb on - strand, within PP_2801at 3192.784 kb on - strand, within PP_2801at 3192.784 kb on - strand, within PP_2801

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Per-strain Table

Position Strand Gene LocusTag Fraction Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=144_hrs
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3,189,775 + PP_2799 0.37 -2.0
3,189,776 - PP_2799 0.37 -1.1
3,189,776 - PP_2799 0.37 -0.5
3,189,827 - PP_2799 0.40 -2.3
3,189,934 + PP_2799 0.48 -0.7
3,190,023 - PP_2799 0.55 -3.4
3,190,023 - PP_2799 0.55 -1.3
3,190,024 + PP_2799 0.55 -2.4
3,190,024 + PP_2799 0.55 -0.3
3,190,024 + PP_2799 0.55 +0.2
3,190,025 - PP_2799 0.55 +0.1
3,190,025 - PP_2799 0.55 +0.2
3,190,025 - PP_2799 0.55 +0.8
3,190,056 - PP_2799 0.57 -2.9
3,190,056 - PP_2799 0.57 +1.6
3,190,201 + PP_2799 0.67 +0.4
3,190,405 + PP_2799 0.82 -2.3
3,190,405 + PP_2799 0.82 -0.1
3,190,408 + PP_2799 0.82 -0.4
3,191,478 + PP_2800 0.58 -1.5
3,191,494 - PP_2800 0.60 -1.0
3,191,583 + PP_2800 0.67 +0.6
3,191,592 + PP_2800 0.68 -1.9
3,191,691 + PP_2800 0.75 -1.5
3,191,841 + PP_2800 0.87 +0.5
3,191,841 + PP_2800 0.87 -2.7
3,191,842 - PP_2800 0.87 -0.8
3,191,878 - -1.1
3,192,003 - -0.5
3,192,070 + +0.1
3,192,085 + -0.5
3,192,086 - -0.1
3,192,086 - -1.3
3,192,429 + PP_2801 0.28 -0.3
3,192,429 + PP_2801 0.28 -0.6
3,192,429 + PP_2801 0.28 +0.2
3,192,430 - PP_2801 0.28 -2.0
3,192,430 - PP_2801 0.28 -3.1
3,192,430 - PP_2801 0.28 -1.0
3,192,430 - PP_2801 0.28 -0.4
3,192,431 + PP_2801 0.28 -1.3
3,192,431 + PP_2801 0.28 -1.4
3,192,601 + PP_2801 0.40 -1.2
3,192,602 - PP_2801 0.40 -0.9
3,192,602 - PP_2801 0.40 -0.5
3,192,641 - PP_2801 0.42 +0.6
3,192,728 + PP_2801 0.49 -1.7
3,192,728 + PP_2801 0.49 +0.6
3,192,729 - PP_2801 0.49 -0.1
3,192,741 - PP_2801 0.49 -1.7
3,192,784 - PP_2801 0.52 -1.4
3,192,784 - PP_2801 0.52 +0.0

Or see this region's nucleotide sequence