Strain Fitness in Pseudomonas putida KT2440 around PP_1422

Experiment: Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=144_hrs

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPP_1421 and PP_1422 are separated by 65 nucleotidesPP_1422 and PP_1423 are separated by 49 nucleotides PP_1421: PP_1421 - putative Sensor histidine kinase TctE, at 1,621,087 to 1,622,478 _1421 PP_1422: PP_1422 - conserved protein of unknown function, at 1,622,544 to 1,623,356 _1422 PP_1423: PP_1423 - conserved protein of unknown function, at 1,623,406 to 1,624,347 _1423 Position (kb) 1622 1623 1624Strain fitness (log2 ratio) -1 0 1 2at 1622.226 kb on + strand, within PP_1421at 1622.226 kb on + strand, within PP_1421at 1622.227 kb on - strand, within PP_1421at 1622.301 kb on + strand, within PP_1421at 1622.341 kb on + strandat 1622.341 kb on + strandat 1622.467 kb on - strandat 1622.539 kb on - strandat 1622.696 kb on - strand, within PP_1422at 1622.821 kb on + strand, within PP_1422at 1622.821 kb on + strand, within PP_1422at 1622.821 kb on + strand, within PP_1422at 1622.822 kb on - strand, within PP_1422at 1622.869 kb on + strand, within PP_1422at 1622.869 kb on + strand, within PP_1422at 1622.869 kb on + strand, within PP_1422at 1622.869 kb on + strand, within PP_1422at 1622.869 kb on + strand, within PP_1422at 1622.870 kb on - strand, within PP_1422at 1622.877 kb on + strand, within PP_1422at 1623.082 kb on + strand, within PP_1422at 1623.220 kb on - strand, within PP_1422at 1623.360 kb on + strandat 1623.360 kb on + strandat 1623.441 kb on - strandat 1623.441 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=144_hrs
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1,622,226 + PP_1421 0.82 +0.4
1,622,226 + PP_1421 0.82 -0.7
1,622,227 - PP_1421 0.82 +0.6
1,622,301 + PP_1421 0.87 +0.8
1,622,341 + -0.6
1,622,341 + -0.9
1,622,467 - +0.6
1,622,539 - +0.1
1,622,696 - PP_1422 0.19 -0.6
1,622,821 + PP_1422 0.34 -0.5
1,622,821 + PP_1422 0.34 +0.2
1,622,821 + PP_1422 0.34 +0.1
1,622,822 - PP_1422 0.34 -0.8
1,622,869 + PP_1422 0.40 +0.7
1,622,869 + PP_1422 0.40 +2.6
1,622,869 + PP_1422 0.40 -0.9
1,622,869 + PP_1422 0.40 -0.4
1,622,869 + PP_1422 0.40 +2.3
1,622,870 - PP_1422 0.40 +0.8
1,622,877 + PP_1422 0.41 +0.7
1,623,082 + PP_1422 0.66 +0.4
1,623,220 - PP_1422 0.83 +1.3
1,623,360 + +0.0
1,623,360 + -1.1
1,623,441 - +0.1
1,623,441 - -0.7

Or see this region's nucleotide sequence