Experiment: Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=72_hrs
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_4826 and PP_4827 overlap by 13 nucleotides PP_4827 and PP_4828 overlap by 4 nucleotides PP_4828 and PP_4829 overlap by 8 nucleotides
PP_4826: PP_4826 - Precorrin-3B C17-methyltransferase, at 5,492,318 to 5,494,015
_4826
PP_4827: PP_4827 - Precorrin-2 C(20)-methyltransferase, at 5,494,003 to 5,494,737
_4827
PP_4828: PP_4828 - Precorrin-8X methylmutase, at 5,494,734 to 5,495,360
_4828
PP_4829: PP_4829 - putative precorrin-3B synthase, at 5,495,353 to 5,496,684
_4829
Position (kb)
5494
5495
5496 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 5493.854 kb on - strand at 5493.940 kb on + strand at 5493.940 kb on + strand at 5494.046 kb on + strand at 5494.118 kb on + strand, within PP_4827 at 5494.118 kb on + strand, within PP_4827 at 5494.119 kb on - strand, within PP_4827 at 5494.119 kb on - strand, within PP_4827 at 5494.119 kb on - strand, within PP_4827 at 5494.119 kb on - strand, within PP_4827 at 5494.119 kb on - strand, within PP_4827 at 5494.119 kb on - strand at 5494.119 kb on - strand, within PP_4827 at 5494.275 kb on + strand, within PP_4827 at 5494.379 kb on + strand, within PP_4827 at 5494.379 kb on + strand, within PP_4827 at 5494.379 kb on + strand, within PP_4827 at 5494.379 kb on + strand, within PP_4827 at 5494.379 kb on + strand, within PP_4827 at 5494.379 kb on + strand, within PP_4827 at 5494.379 kb on + strand, within PP_4827 at 5494.379 kb on + strand, within PP_4827 at 5494.380 kb on - strand, within PP_4827 at 5494.380 kb on - strand, within PP_4827 at 5494.380 kb on - strand, within PP_4827 at 5494.380 kb on - strand, within PP_4827 at 5494.380 kb on - strand, within PP_4827 at 5494.380 kb on - strand, within PP_4827 at 5494.385 kb on + strand, within PP_4827 at 5494.385 kb on + strand, within PP_4827 at 5494.385 kb on + strand, within PP_4827 at 5494.385 kb on + strand, within PP_4827 at 5494.385 kb on + strand, within PP_4827 at 5494.385 kb on + strand, within PP_4827 at 5494.385 kb on + strand, within PP_4827 at 5494.385 kb on + strand, within PP_4827 at 5494.385 kb on + strand, within PP_4827 at 5494.386 kb on - strand, within PP_4827 at 5494.386 kb on - strand, within PP_4827 at 5494.386 kb on - strand, within PP_4827 at 5494.386 kb on - strand, within PP_4827 at 5494.386 kb on - strand, within PP_4827 at 5494.386 kb on - strand, within PP_4827 at 5494.394 kb on + strand, within PP_4827 at 5494.395 kb on - strand, within PP_4827 at 5494.395 kb on - strand, within PP_4827 at 5494.462 kb on + strand, within PP_4827 at 5494.462 kb on + strand, within PP_4827 at 5494.462 kb on + strand, within PP_4827 at 5494.463 kb on - strand, within PP_4827 at 5494.463 kb on - strand, within PP_4827 at 5494.463 kb on - strand, within PP_4827 at 5494.467 kb on + strand, within PP_4827 at 5494.467 kb on + strand, within PP_4827 at 5494.478 kb on + strand, within PP_4827 at 5494.479 kb on - strand, within PP_4827 at 5494.503 kb on - strand, within PP_4827 at 5494.503 kb on - strand, within PP_4827 at 5494.503 kb on - strand, within PP_4827 at 5494.503 kb on - strand, within PP_4827 at 5494.588 kb on - strand, within PP_4827 at 5494.628 kb on + strand, within PP_4827 at 5494.937 kb on - strand, within PP_4828 at 5495.011 kb on - strand, within PP_4828 at 5495.326 kb on + strand at 5495.327 kb on - strand at 5495.327 kb on - strand at 5495.351 kb on - strand at 5495.410 kb on - strand at 5495.462 kb on + strand at 5495.462 kb on + strand at 5495.462 kb on + strand at 5495.463 kb on - strand at 5495.658 kb on + strand, within PP_4829 at 5495.659 kb on - strand, within PP_4829 at 5495.808 kb on + strand, within PP_4829 at 5495.808 kb on + strand, within PP_4829 at 5495.996 kb on + strand, within PP_4829 at 5495.996 kb on + strand, within PP_4829
Per-strain Table
Position Strand Gene LocusTag Fraction Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=72_hrs remove 5,493,854 - -1.6 5,493,940 + +1.0 5,493,940 + +2.5 5,494,046 + +0.1 5,494,118 + PP_4827 0.16 +0.1 5,494,118 + PP_4827 0.16 +0.4 5,494,119 - PP_4827 0.16 -0.1 5,494,119 - PP_4827 0.16 -0.2 5,494,119 - PP_4827 0.16 +1.0 5,494,119 - PP_4827 0.16 -0.2 5,494,119 - PP_4827 0.16 -0.9 5,494,119 - -0.2 5,494,119 - PP_4827 0.16 +1.5 5,494,275 + PP_4827 0.37 +1.8 5,494,379 + PP_4827 0.51 +0.4 5,494,379 + PP_4827 0.51 +0.3 5,494,379 + PP_4827 0.51 -1.6 5,494,379 + PP_4827 0.51 +0.9 5,494,379 + PP_4827 0.51 +0.4 5,494,379 + PP_4827 0.51 -0.9 5,494,379 + PP_4827 0.51 +0.1 5,494,379 + PP_4827 0.51 +0.5 5,494,380 - PP_4827 0.51 +0.8 5,494,380 - PP_4827 0.51 -0.6 5,494,380 - PP_4827 0.51 -0.3 5,494,380 - PP_4827 0.51 -1.0 5,494,380 - PP_4827 0.51 -1.2 5,494,380 - PP_4827 0.51 +0.8 5,494,385 + PP_4827 0.52 -0.5 5,494,385 + PP_4827 0.52 -0.2 5,494,385 + PP_4827 0.52 -0.1 5,494,385 + PP_4827 0.52 +0.9 5,494,385 + PP_4827 0.52 +0.2 5,494,385 + PP_4827 0.52 -0.2 5,494,385 + PP_4827 0.52 -2.0 5,494,385 + PP_4827 0.52 -1.2 5,494,385 + PP_4827 0.52 +1.6 5,494,386 - PP_4827 0.52 -0.1 5,494,386 - PP_4827 0.52 +1.3 5,494,386 - PP_4827 0.52 -0.7 5,494,386 - PP_4827 0.52 +1.3 5,494,386 - PP_4827 0.52 -0.4 5,494,386 - PP_4827 0.52 +0.3 5,494,394 + PP_4827 0.53 -0.5 5,494,395 - PP_4827 0.53 -0.4 5,494,395 - PP_4827 0.53 -0.2 5,494,462 + PP_4827 0.62 +0.0 5,494,462 + PP_4827 0.62 +0.7 5,494,462 + PP_4827 0.62 -0.4 5,494,463 - PP_4827 0.63 -0.5 5,494,463 - PP_4827 0.63 +1.3 5,494,463 - PP_4827 0.63 +0.7 5,494,467 + PP_4827 0.63 -0.7 5,494,467 + PP_4827 0.63 -0.7 5,494,478 + PP_4827 0.65 -0.1 5,494,479 - PP_4827 0.65 +0.8 5,494,503 - PP_4827 0.68 -0.1 5,494,503 - PP_4827 0.68 +1.1 5,494,503 - PP_4827 0.68 -0.2 5,494,503 - PP_4827 0.68 +0.4 5,494,588 - PP_4827 0.80 +1.5 5,494,628 + PP_4827 0.85 +0.1 5,494,937 - PP_4828 0.32 +0.5 5,495,011 - PP_4828 0.44 +0.5 5,495,326 + -1.9 5,495,327 - +1.6 5,495,327 - -1.2 5,495,351 - -1.5 5,495,410 - +0.6 5,495,462 + +0.4 5,495,462 + +2.6 5,495,462 + -3.1 5,495,463 - +0.0 5,495,658 + PP_4829 0.23 +1.1 5,495,659 - PP_4829 0.23 +0.4 5,495,808 + PP_4829 0.34 -0.2 5,495,808 + PP_4829 0.34 -1.4 5,495,996 + PP_4829 0.48 +0.3 5,495,996 + PP_4829 0.48 +0.8
Or see this region's nucleotide sequence