Strain Fitness in Pseudomonas putida KT2440 around PP_1048

Experiment: Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=72_hrs

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPP_1047 and PP_1048 are separated by 3 nucleotidesPP_1048 and PP_1049 overlap by 4 nucleotidesPP_1049 and PP_1050 are separated by 3 nucleotidesPP_1050 and PP_1051 overlap by 43 nucleotides PP_1047: PP_1047 - putative protein secretion protein for export, at 1,197,691 to 1,199,142 _1047 PP_1048: PP_1048 - putative protein secretion protein for export, at 1,199,146 to 1,200,348 _1048 PP_1049: PP_1049 - putative protein secretion protein for export, at 1,200,345 to 1,200,800 _1049 PP_1050: PP_1050 - Type II secretion pathway protein XcpU, at 1,200,804 to 1,201,235 _1050 PP_1051: PP_1051 - Type II secretion pathway protein XcpV, at 1,201,193 to 1,201,600 _1051 Position (kb) 1199 1200 1201Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 1198.166 kb on + strand, within PP_1047at 1198.266 kb on - strand, within PP_1047at 1198.373 kb on + strand, within PP_1047at 1198.374 kb on - strand, within PP_1047at 1198.374 kb on - strand, within PP_1047at 1198.462 kb on + strand, within PP_1047at 1198.463 kb on - strand, within PP_1047at 1198.730 kb on + strand, within PP_1047at 1198.874 kb on + strandat 1198.874 kb on + strand, within PP_1047at 1198.874 kb on + strand, within PP_1047at 1198.875 kb on - strandat 1198.906 kb on + strand, within PP_1047at 1198.948 kb on - strand, within PP_1047at 1198.967 kb on - strand, within PP_1047at 1198.967 kb on - strandat 1198.967 kb on - strand, within PP_1047at 1199.008 kb on + strandat 1199.008 kb on + strandat 1199.009 kb on - strandat 1199.041 kb on + strandat 1199.042 kb on - strandat 1199.042 kb on - strandat 1199.140 kb on + strandat 1199.155 kb on + strandat 1199.156 kb on - strandat 1199.161 kb on + strandat 1199.524 kb on + strand, within PP_1048at 1199.525 kb on - strand, within PP_1048at 1199.644 kb on + strand, within PP_1048at 1200.359 kb on + strandat 1200.360 kb on - strandat 1200.360 kb on - strandat 1200.360 kb on - strandat 1200.428 kb on + strand, within PP_1049at 1200.428 kb on + strand, within PP_1049at 1200.543 kb on + strand, within PP_1049at 1200.543 kb on + strand, within PP_1049at 1200.544 kb on - strandat 1200.544 kb on - strand, within PP_1049at 1200.544 kb on - strand, within PP_1049at 1200.544 kb on - strand, within PP_1049at 1200.648 kb on + strand, within PP_1049at 1200.649 kb on - strand, within PP_1049at 1200.688 kb on - strand, within PP_1049at 1200.688 kb on - strand, within PP_1049at 1200.730 kb on - strand, within PP_1049at 1200.798 kb on + strandat 1200.799 kb on - strandat 1201.157 kb on + strand, within PP_1050at 1201.275 kb on + strand, within PP_1051at 1201.320 kb on + strand, within PP_1051at 1201.321 kb on - strand, within PP_1051

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Per-strain Table

Position Strand Gene LocusTag Fraction Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=72_hrs
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1,198,166 + PP_1047 0.33 +0.4
1,198,266 - PP_1047 0.40 +0.5
1,198,373 + PP_1047 0.47 +0.7
1,198,374 - PP_1047 0.47 -0.1
1,198,374 - PP_1047 0.47 -0.6
1,198,462 + PP_1047 0.53 +0.1
1,198,463 - PP_1047 0.53 +0.2
1,198,730 + PP_1047 0.72 -0.5
1,198,874 + +0.1
1,198,874 + PP_1047 0.81 +0.3
1,198,874 + PP_1047 0.81 -0.2
1,198,875 - +2.1
1,198,906 + PP_1047 0.84 +1.0
1,198,948 - PP_1047 0.87 +0.8
1,198,967 - PP_1047 0.88 +0.5
1,198,967 - +3.1
1,198,967 - PP_1047 0.88 -0.3
1,199,008 + -0.5
1,199,008 + -0.2
1,199,009 - -0.0
1,199,041 + +0.1
1,199,042 - +0.3
1,199,042 - +0.7
1,199,140 + -1.1
1,199,155 + -0.2
1,199,156 - +0.7
1,199,161 + -0.6
1,199,524 + PP_1048 0.31 +0.2
1,199,525 - PP_1048 0.32 +0.0
1,199,644 + PP_1048 0.41 +0.9
1,200,359 + +0.9
1,200,360 - -0.7
1,200,360 - +0.9
1,200,360 - -0.3
1,200,428 + PP_1049 0.18 -0.3
1,200,428 + PP_1049 0.18 -0.1
1,200,543 + PP_1049 0.43 -2.9
1,200,543 + PP_1049 0.43 +0.2
1,200,544 - +0.1
1,200,544 - PP_1049 0.44 +1.0
1,200,544 - PP_1049 0.44 -0.5
1,200,544 - PP_1049 0.44 -1.7
1,200,648 + PP_1049 0.66 -1.4
1,200,649 - PP_1049 0.67 -0.2
1,200,688 - PP_1049 0.75 +0.1
1,200,688 - PP_1049 0.75 +1.3
1,200,730 - PP_1049 0.84 +0.2
1,200,798 + +0.8
1,200,799 - +0.1
1,201,157 + PP_1050 0.82 -0.1
1,201,275 + PP_1051 0.20 -0.5
1,201,320 + PP_1051 0.31 -0.9
1,201,321 - PP_1051 0.31 -0.1

Or see this region's nucleotide sequence