Experiment: JP1 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_26450 and PS417_26455 are separated by 210 nucleotides PS417_26455 and PS417_26460 are separated by 95 nucleotides PS417_26460 and PS417_26465 are separated by 39 nucleotides PS417_26465 and PS417_26470 are separated by 6 nucleotides
PS417_26450: PS417_26450 - S-adenosylmethionine synthetase, at 5,720,606 to 5,721,796
_26450
PS417_26455: PS417_26455 - hypothetical protein, at 5,722,007 to 5,722,357
_26455
PS417_26460: PS417_26460 - NAD-dependent DNA ligase LigB, at 5,722,453 to 5,724,117
_26460
PS417_26465: PS417_26465 - hypothetical protein, at 5,724,157 to 5,724,552
_26465
PS417_26470: PS417_26470 - cytochrome C, at 5,724,559 to 5,725,008
_26470
Position (kb)
5722
5723
5724
5725 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 5721.795 kb on - strand at 5721.846 kb on + strand at 5721.846 kb on + strand at 5721.894 kb on - strand at 5721.934 kb on - strand at 5721.934 kb on - strand at 5721.934 kb on - strand at 5721.938 kb on + strand at 5722.315 kb on + strand, within PS417_26455 at 5722.355 kb on + strand at 5722.356 kb on - strand at 5722.359 kb on + strand at 5722.360 kb on - strand at 5722.365 kb on + strand at 5722.365 kb on + strand at 5722.372 kb on + strand at 5722.421 kb on - strand at 5722.478 kb on + strand at 5722.479 kb on - strand at 5722.562 kb on + strand at 5722.562 kb on + strand at 5722.562 kb on + strand at 5722.562 kb on + strand at 5722.582 kb on + strand at 5722.582 kb on + strand at 5722.582 kb on + strand at 5722.583 kb on - strand at 5722.621 kb on + strand, within PS417_26460 at 5722.622 kb on - strand, within PS417_26460 at 5722.622 kb on - strand, within PS417_26460 at 5722.738 kb on - strand, within PS417_26460 at 5723.249 kb on - strand, within PS417_26460 at 5723.396 kb on + strand, within PS417_26460 at 5723.397 kb on - strand, within PS417_26460 at 5723.741 kb on + strand, within PS417_26460 at 5723.742 kb on - strand, within PS417_26460 at 5723.762 kb on + strand, within PS417_26460 at 5723.946 kb on + strand, within PS417_26460 at 5723.947 kb on - strand, within PS417_26460 at 5724.068 kb on + strand at 5724.068 kb on + strand at 5724.069 kb on - strand at 5724.069 kb on - strand at 5724.090 kb on - strand at 5724.490 kb on - strand, within PS417_26465 at 5724.641 kb on + strand, within PS417_26470 at 5724.642 kb on - strand, within PS417_26470 at 5724.983 kb on - strand at 5724.983 kb on - strand at 5725.075 kb on + strand at 5725.075 kb on + strand at 5725.078 kb on + strand at 5725.079 kb on - strand at 5725.081 kb on + strand at 5725.081 kb on + strand at 5725.082 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction JP1 MOI 0.1 remove 5,721,795 - -0.0 5,721,846 + +0.8 5,721,846 + +0.3 5,721,894 - -0.1 5,721,934 - -1.1 5,721,934 - -0.1 5,721,934 - -0.1 5,721,938 + +0.3 5,722,315 + PS417_26455 0.88 -0.3 5,722,355 + -0.9 5,722,356 - -0.0 5,722,359 + -1.1 5,722,360 - +1.7 5,722,365 + +0.1 5,722,365 + +1.9 5,722,372 + +0.0 5,722,421 - -3.2 5,722,478 + +0.3 5,722,479 - -0.7 5,722,562 + +0.1 5,722,562 + +2.9 5,722,562 + -0.2 5,722,562 + -0.9 5,722,582 + +2.1 5,722,582 + -1.7 5,722,582 + +1.1 5,722,583 - +0.4 5,722,621 + PS417_26460 0.10 +0.3 5,722,622 - PS417_26460 0.10 -0.2 5,722,622 - PS417_26460 0.10 -1.7 5,722,738 - PS417_26460 0.17 -1.5 5,723,249 - PS417_26460 0.48 -0.8 5,723,396 + PS417_26460 0.57 +1.6 5,723,397 - PS417_26460 0.57 -1.6 5,723,741 + PS417_26460 0.77 +1.0 5,723,742 - PS417_26460 0.77 -0.5 5,723,762 + PS417_26460 0.79 -1.1 5,723,946 + PS417_26460 0.90 -0.3 5,723,947 - PS417_26460 0.90 -1.5 5,724,068 + +0.0 5,724,068 + -0.1 5,724,069 - -1.1 5,724,069 - -1.4 5,724,090 - -0.6 5,724,490 - PS417_26465 0.84 -0.3 5,724,641 + PS417_26470 0.18 +0.8 5,724,642 - PS417_26470 0.18 +0.7 5,724,983 - -0.2 5,724,983 - -0.4 5,725,075 + -0.0 5,725,075 + -0.7 5,725,078 + +1.9 5,725,079 - +0.8 5,725,081 + +1.0 5,725,081 + -0.9 5,725,082 - +0.6
Or see this region's nucleotide sequence