Experiment: JP1 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_23515 and PS417_23520 are separated by 283 nucleotides PS417_23520 and PS417_23525 are separated by 54 nucleotides
PS417_23515: PS417_23515 - thioredoxin reductase, at 5,087,627 to 5,088,589
_23515
PS417_23520: PS417_23520 - cysteine biosynthesis protein CysZ, at 5,088,873 to 5,089,640
_23520
PS417_23525: PS417_23525 - glycoside hydrolase, at 5,089,695 to 5,090,897
_23525
Position (kb)
5088
5089
5090 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 5088.617 kb on - strand at 5088.689 kb on - strand at 5088.736 kb on - strand at 5088.736 kb on - strand at 5088.751 kb on - strand at 5089.003 kb on - strand, within PS417_23520 at 5089.081 kb on - strand, within PS417_23520 at 5089.192 kb on + strand, within PS417_23520 at 5089.192 kb on + strand, within PS417_23520 at 5089.192 kb on + strand, within PS417_23520 at 5089.192 kb on + strand, within PS417_23520 at 5089.193 kb on - strand, within PS417_23520 at 5089.193 kb on - strand, within PS417_23520 at 5089.639 kb on - strand at 5089.981 kb on - strand, within PS417_23525 at 5089.981 kb on - strand, within PS417_23525 at 5090.080 kb on - strand, within PS417_23525 at 5090.080 kb on - strand, within PS417_23525 at 5090.127 kb on + strand, within PS417_23525 at 5090.128 kb on - strand, within PS417_23525 at 5090.145 kb on - strand, within PS417_23525 at 5090.300 kb on - strand, within PS417_23525 at 5090.326 kb on - strand, within PS417_23525 at 5090.326 kb on - strand, within PS417_23525 at 5090.397 kb on + strand, within PS417_23525
Per-strain Table
Position Strand Gene LocusTag Fraction JP1 MOI 0.1 remove 5,088,617 - +2.8 5,088,689 - -0.5 5,088,736 - +0.2 5,088,736 - +0.8 5,088,751 - +0.9 5,089,003 - PS417_23520 0.17 +1.6 5,089,081 - PS417_23520 0.27 -2.2 5,089,192 + PS417_23520 0.42 -1.6 5,089,192 + PS417_23520 0.42 +0.8 5,089,192 + PS417_23520 0.42 +1.8 5,089,192 + PS417_23520 0.42 +1.9 5,089,193 - PS417_23520 0.42 +0.4 5,089,193 - PS417_23520 0.42 -2.0 5,089,639 - -0.5 5,089,981 - PS417_23525 0.24 +0.0 5,089,981 - PS417_23525 0.24 +0.1 5,090,080 - PS417_23525 0.32 +0.1 5,090,080 - PS417_23525 0.32 -0.6 5,090,127 + PS417_23525 0.36 +0.2 5,090,128 - PS417_23525 0.36 +0.1 5,090,145 - PS417_23525 0.37 +0.8 5,090,300 - PS417_23525 0.50 -0.2 5,090,326 - PS417_23525 0.52 +1.9 5,090,326 - PS417_23525 0.52 -0.6 5,090,397 + PS417_23525 0.58 -1.9
Or see this region's nucleotide sequence