Experiment: JP1 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_12955 and PS417_12960 are separated by 317 nucleotides PS417_12960 and PS417_12965 are separated by 189 nucleotides PS417_12965 and PS417_12970 are separated by 59 nucleotides PS417_12970 and PS417_12975 overlap by 4 nucleotides
PS417_12955: PS417_12955 - sulfonate ABC transporter substrate-binding protein, at 2,831,790 to 2,832,746
_12955
PS417_12960: PS417_12960 - transporter, at 2,833,064 to 2,833,279
_12960
PS417_12965: PS417_12965 - aminotransferase, at 2,833,469 to 2,834,620
_12965
PS417_12970: PS417_12970 - FMN-dependent NADH-azoreductase, at 2,834,680 to 2,835,273
_12970
PS417_12975: PS417_12975 - GCN5 family acetyltransferase, at 2,835,270 to 2,835,710
_12975
Position (kb)
2833
2834
2835 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2832.471 kb on + strand, within PS417_12955 at 2832.524 kb on - strand, within PS417_12955 at 2832.632 kb on - strand, within PS417_12955 at 2832.634 kb on + strand, within PS417_12955 at 2832.723 kb on + strand at 2832.858 kb on + strand at 2833.281 kb on + strand at 2833.281 kb on + strand at 2833.674 kb on + strand, within PS417_12965 at 2833.674 kb on + strand, within PS417_12965 at 2833.675 kb on - strand, within PS417_12965 at 2833.675 kb on - strand, within PS417_12965 at 2833.678 kb on + strand, within PS417_12965 at 2833.679 kb on - strand, within PS417_12965 at 2833.679 kb on - strand, within PS417_12965 at 2833.679 kb on - strand, within PS417_12965 at 2833.679 kb on - strand, within PS417_12965 at 2833.679 kb on - strand, within PS417_12965 at 2833.929 kb on + strand, within PS417_12965 at 2834.196 kb on + strand, within PS417_12965 at 2834.429 kb on + strand, within PS417_12965 at 2834.429 kb on + strand at 2834.631 kb on + strand at 2834.682 kb on - strand at 2834.684 kb on - strand at 2834.805 kb on + strand, within PS417_12970 at 2834.806 kb on - strand, within PS417_12970 at 2834.825 kb on + strand, within PS417_12970 at 2834.825 kb on + strand, within PS417_12970 at 2834.826 kb on - strand, within PS417_12970 at 2834.826 kb on - strand, within PS417_12970 at 2835.004 kb on - strand, within PS417_12970 at 2835.120 kb on - strand, within PS417_12970 at 2835.155 kb on + strand, within PS417_12970 at 2835.350 kb on + strand, within PS417_12975 at 2835.466 kb on - strand, within PS417_12975 at 2835.478 kb on + strand, within PS417_12975 at 2835.479 kb on - strand, within PS417_12975 at 2835.482 kb on + strand, within PS417_12975 at 2835.483 kb on - strand, within PS417_12975 at 2835.493 kb on + strand, within PS417_12975 at 2835.494 kb on - strand, within PS417_12975 at 2835.494 kb on - strand, within PS417_12975
Per-strain Table
Position Strand Gene LocusTag Fraction JP1 MOI 0.1 remove 2,832,471 + PS417_12955 0.71 +0.4 2,832,524 - PS417_12955 0.77 -1.8 2,832,632 - PS417_12955 0.88 +0.1 2,832,634 + PS417_12955 0.88 -0.4 2,832,723 + -1.0 2,832,858 + -1.1 2,833,281 + +1.1 2,833,281 + -1.3 2,833,674 + PS417_12965 0.18 +0.7 2,833,674 + PS417_12965 0.18 -0.7 2,833,675 - PS417_12965 0.18 -0.3 2,833,675 - PS417_12965 0.18 -0.9 2,833,678 + PS417_12965 0.18 +0.0 2,833,679 - PS417_12965 0.18 -0.3 2,833,679 - PS417_12965 0.18 -0.6 2,833,679 - PS417_12965 0.18 -0.3 2,833,679 - PS417_12965 0.18 -0.6 2,833,679 - PS417_12965 0.18 -0.5 2,833,929 + PS417_12965 0.40 -0.3 2,834,196 + PS417_12965 0.63 -0.6 2,834,429 + PS417_12965 0.83 -1.5 2,834,429 + +0.7 2,834,631 + -1.7 2,834,682 - +0.3 2,834,684 - +0.1 2,834,805 + PS417_12970 0.21 -1.6 2,834,806 - PS417_12970 0.21 -1.8 2,834,825 + PS417_12970 0.24 +0.7 2,834,825 + PS417_12970 0.24 -1.2 2,834,826 - PS417_12970 0.25 -0.7 2,834,826 - PS417_12970 0.25 -0.7 2,835,004 - PS417_12970 0.55 -0.1 2,835,120 - PS417_12970 0.74 +0.7 2,835,155 + PS417_12970 0.80 +2.1 2,835,350 + PS417_12975 0.18 +0.4 2,835,466 - PS417_12975 0.44 -1.3 2,835,478 + PS417_12975 0.47 -0.2 2,835,479 - PS417_12975 0.47 -0.3 2,835,482 + PS417_12975 0.48 -0.3 2,835,483 - PS417_12975 0.48 +0.4 2,835,493 + PS417_12975 0.51 -1.1 2,835,494 - PS417_12975 0.51 -0.2 2,835,494 - PS417_12975 0.51 -1.7
Or see this region's nucleotide sequence