Experiment: JP1 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_12610 and PS417_12615 are separated by 170 nucleotides PS417_12615 and PS417_12620 are separated by 138 nucleotides PS417_12620 and PS417_12625 are separated by 91 nucleotides
PS417_12610: PS417_12610 - RNA polymerase sigma factor, at 2,745,135 to 2,745,650
_12610
PS417_12615: PS417_12615 - hypothetical protein, at 2,745,821 to 2,746,342
_12615
PS417_12620: PS417_12620 - SAM-dependent methyltransferase, at 2,746,481 to 2,747,251
_12620
PS417_12625: PS417_12625 - membrane protein, at 2,747,343 to 2,748,128
_12625
Position (kb)
2746
2747
2748 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2745.583 kb on + strand at 2745.584 kb on - strand, within PS417_12610 at 2745.652 kb on + strand at 2745.652 kb on + strand at 2745.652 kb on + strand at 2745.653 kb on - strand at 2745.653 kb on - strand at 2745.653 kb on - strand at 2745.684 kb on - strand at 2745.684 kb on - strand at 2745.684 kb on - strand at 2745.777 kb on + strand at 2745.777 kb on + strand at 2745.784 kb on + strand at 2745.784 kb on + strand at 2745.784 kb on + strand at 2745.784 kb on + strand at 2745.784 kb on + strand at 2745.784 kb on + strand at 2745.785 kb on - strand at 2745.785 kb on - strand at 2745.785 kb on - strand at 2745.785 kb on - strand at 2745.785 kb on - strand at 2745.785 kb on - strand at 2745.785 kb on - strand at 2745.808 kb on + strand at 2745.808 kb on + strand at 2745.943 kb on - strand, within PS417_12615 at 2746.095 kb on + strand, within PS417_12615 at 2746.095 kb on + strand, within PS417_12615 at 2746.198 kb on - strand, within PS417_12615 at 2746.198 kb on - strand, within PS417_12615 at 2746.312 kb on + strand at 2746.334 kb on - strand at 2746.347 kb on - strand at 2746.362 kb on + strand at 2746.491 kb on + strand at 2746.491 kb on + strand at 2746.492 kb on - strand at 2746.492 kb on - strand at 2746.492 kb on - strand at 2746.946 kb on - strand, within PS417_12620 at 2747.035 kb on - strand, within PS417_12620 at 2747.035 kb on - strand, within PS417_12620 at 2747.228 kb on + strand at 2747.228 kb on + strand at 2747.228 kb on + strand at 2747.229 kb on - strand at 2747.229 kb on - strand at 2747.229 kb on - strand at 2747.229 kb on - strand at 2747.278 kb on - strand at 2747.280 kb on - strand at 2747.323 kb on + strand at 2747.335 kb on + strand at 2747.336 kb on - strand at 2747.336 kb on - strand at 2747.394 kb on - strand at 2747.493 kb on + strand, within PS417_12625 at 2747.546 kb on + strand, within PS417_12625 at 2747.677 kb on + strand, within PS417_12625 at 2747.757 kb on + strand, within PS417_12625 at 2747.757 kb on + strand, within PS417_12625 at 2747.758 kb on - strand, within PS417_12625 at 2747.874 kb on - strand at 2748.030 kb on + strand, within PS417_12625 at 2748.030 kb on + strand, within PS417_12625 at 2748.030 kb on + strand, within PS417_12625 at 2748.030 kb on + strand, within PS417_12625 at 2748.030 kb on + strand, within PS417_12625 at 2748.031 kb on - strand, within PS417_12625 at 2748.031 kb on - strand, within PS417_12625 at 2748.031 kb on - strand, within PS417_12625 at 2748.031 kb on - strand, within PS417_12625 at 2748.044 kb on + strand, within PS417_12625 at 2748.045 kb on - strand, within PS417_12625 at 2748.111 kb on + strand at 2748.224 kb on + strand at 2748.225 kb on - strand at 2748.233 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction JP1 MOI 0.1 remove 2,745,583 + +3.3 2,745,584 - PS417_12610 0.87 -0.4 2,745,652 + +0.1 2,745,652 + +0.5 2,745,652 + +0.7 2,745,653 - +0.3 2,745,653 - -0.5 2,745,653 - -0.2 2,745,684 - +1.7 2,745,684 - -2.1 2,745,684 - +0.1 2,745,777 + -3.4 2,745,777 + +0.3 2,745,784 + -0.0 2,745,784 + -0.1 2,745,784 + -0.3 2,745,784 + +1.2 2,745,784 + -0.3 2,745,784 + -0.4 2,745,785 - -0.5 2,745,785 - -0.5 2,745,785 - -0.7 2,745,785 - -1.1 2,745,785 - +1.1 2,745,785 - -0.4 2,745,785 - -0.5 2,745,808 + +0.1 2,745,808 + +1.1 2,745,943 - PS417_12615 0.23 -0.7 2,746,095 + PS417_12615 0.52 -1.6 2,746,095 + PS417_12615 0.52 +0.3 2,746,198 - PS417_12615 0.72 -0.6 2,746,198 - PS417_12615 0.72 +1.7 2,746,312 + +0.1 2,746,334 - +0.6 2,746,347 - +2.6 2,746,362 + -1.1 2,746,491 + -2.0 2,746,491 + -1.4 2,746,492 - -2.3 2,746,492 - -1.1 2,746,492 - -0.2 2,746,946 - PS417_12620 0.60 -0.4 2,747,035 - PS417_12620 0.72 -0.3 2,747,035 - PS417_12620 0.72 -1.3 2,747,228 + -0.6 2,747,228 + -0.0 2,747,228 + +0.6 2,747,229 - -1.1 2,747,229 - +0.1 2,747,229 - +0.2 2,747,229 - -1.6 2,747,278 - +1.7 2,747,280 - +0.6 2,747,323 + -0.1 2,747,335 + +2.2 2,747,336 - +0.4 2,747,336 - +0.3 2,747,394 - -1.9 2,747,493 + PS417_12625 0.19 +0.5 2,747,546 + PS417_12625 0.26 +0.7 2,747,677 + PS417_12625 0.42 -2.0 2,747,757 + PS417_12625 0.53 -0.2 2,747,757 + PS417_12625 0.53 +0.7 2,747,758 - PS417_12625 0.53 -0.9 2,747,874 - +1.7 2,748,030 + PS417_12625 0.87 -1.0 2,748,030 + PS417_12625 0.87 +0.0 2,748,030 + PS417_12625 0.87 -0.3 2,748,030 + PS417_12625 0.87 -0.1 2,748,030 + PS417_12625 0.87 -1.1 2,748,031 - PS417_12625 0.88 +2.7 2,748,031 - PS417_12625 0.88 -0.4 2,748,031 - PS417_12625 0.88 +1.4 2,748,031 - PS417_12625 0.88 +0.0 2,748,044 + PS417_12625 0.89 -0.3 2,748,045 - PS417_12625 0.89 +0.2 2,748,111 + +0.4 2,748,224 + -1.1 2,748,225 - -0.7 2,748,233 + -1.2
Or see this region's nucleotide sequence