Experiment: JP1 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_00725 and PS417_00730 are separated by 81 nucleotides PS417_00730 and PS417_00735 are separated by 147 nucleotides PS417_00735 and PS417_00740 are separated by 300 nucleotides
PS417_00725: PS417_00725 - RND transporter, at 150,688 to 152,130
_00725
PS417_00730: PS417_00730 - NADH:ubiquinone oxidoreductase subunit N, at 152,212 to 152,844
_00730
PS417_00735: PS417_00735 - hypothetical protein, at 152,992 to 154,488
_00735
PS417_00740: PS417_00740 - membrane protein, at 154,789 to 157,269
_00740
Position (kb) 152
153
154
155 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 152.000 kb on - strand at 152.141 kb on - strand at 152.224 kb on + strand at 152.224 kb on + strand at 152.261 kb on + strand at 152.278 kb on + strand, within PS417_00730 at 152.278 kb on + strand, within PS417_00730 at 152.278 kb on + strand, within PS417_00730 at 152.279 kb on - strand, within PS417_00730 at 152.389 kb on + strand, within PS417_00730 at 152.497 kb on + strand, within PS417_00730 at 152.512 kb on + strand, within PS417_00730 at 152.513 kb on - strand, within PS417_00730 at 152.698 kb on + strand, within PS417_00730 at 152.836 kb on + strand at 152.837 kb on - strand at 152.899 kb on + strand at 152.899 kb on + strand at 152.997 kb on + strand at 152.997 kb on + strand at 153.009 kb on - strand at 153.009 kb on - strand at 153.064 kb on + strand at 153.282 kb on + strand, within PS417_00735 at 153.283 kb on - strand, within PS417_00735 at 153.356 kb on - strand, within PS417_00735 at 153.442 kb on + strand, within PS417_00735 at 153.443 kb on - strand, within PS417_00735 at 153.578 kb on - strand, within PS417_00735 at 153.579 kb on + strand, within PS417_00735 at 153.579 kb on + strand, within PS417_00735 at 153.580 kb on - strand, within PS417_00735 at 153.580 kb on - strand, within PS417_00735 at 153.688 kb on + strand, within PS417_00735 at 153.827 kb on - strand, within PS417_00735 at 153.935 kb on - strand, within PS417_00735 at 153.935 kb on - strand, within PS417_00735 at 154.125 kb on - strand, within PS417_00735 at 154.370 kb on + strand at 154.371 kb on - strand at 154.388 kb on - strand at 154.388 kb on - strand at 154.486 kb on + strand at 154.491 kb on - strand at 154.511 kb on + strand at 154.512 kb on - strand at 154.612 kb on + strand at 154.648 kb on - strand at 154.681 kb on + strand at 154.682 kb on - strand at 154.682 kb on - strand at 154.689 kb on - strand at 154.699 kb on + strand at 154.699 kb on + strand at 154.699 kb on + strand at 154.699 kb on + strand at 154.700 kb on - strand at 154.752 kb on - strand at 154.752 kb on - strand at 154.752 kb on - strand at 154.773 kb on + strand at 154.788 kb on + strand at 154.789 kb on - strand at 154.898 kb on - strand at 155.410 kb on + strand, within PS417_00740 at 155.411 kb on - strand, within PS417_00740 at 155.411 kb on - strand, within PS417_00740
Per-strain Table
Position Strand Gene LocusTag Fraction JP1 MOI 0.1 remove 152,000 - -2.1 152,141 - +0.6 152,224 + -0.3 152,224 + -2.1 152,261 + -0.2 152,278 + PS417_00730 0.10 -1.0 152,278 + PS417_00730 0.10 -1.7 152,278 + PS417_00730 0.10 +0.3 152,279 - PS417_00730 0.11 -0.1 152,389 + PS417_00730 0.28 +1.4 152,497 + PS417_00730 0.45 -1.0 152,512 + PS417_00730 0.47 +1.3 152,513 - PS417_00730 0.48 -0.7 152,698 + PS417_00730 0.77 -0.5 152,836 + +0.3 152,837 - +0.4 152,899 + +0.7 152,899 + +0.1 152,997 + -0.2 152,997 + +0.1 153,009 - +0.8 153,009 - +0.6 153,064 + -1.1 153,282 + PS417_00735 0.19 -1.3 153,283 - PS417_00735 0.19 +0.9 153,356 - PS417_00735 0.24 +0.3 153,442 + PS417_00735 0.30 +0.9 153,443 - PS417_00735 0.30 +0.3 153,578 - PS417_00735 0.39 +1.3 153,579 + PS417_00735 0.39 -0.6 153,579 + PS417_00735 0.39 -0.5 153,580 - PS417_00735 0.39 -0.6 153,580 - PS417_00735 0.39 +2.2 153,688 + PS417_00735 0.46 -3.3 153,827 - PS417_00735 0.56 -1.3 153,935 - PS417_00735 0.63 +1.2 153,935 - PS417_00735 0.63 +1.3 154,125 - PS417_00735 0.76 +0.7 154,370 + +0.9 154,371 - -3.5 154,388 - -0.7 154,388 - -0.3 154,486 + +0.1 154,491 - -0.2 154,511 + -1.1 154,512 - +1.4 154,612 + +2.3 154,648 - -0.5 154,681 + -0.3 154,682 - -0.4 154,682 - +0.9 154,689 - -1.3 154,699 + -1.2 154,699 + -0.0 154,699 + +3.3 154,699 + -0.1 154,700 - +0.5 154,752 - -0.6 154,752 - +0.1 154,752 - +1.6 154,773 + -1.7 154,788 + -0.7 154,789 - -1.9 154,898 - -0.6 155,410 + PS417_00740 0.25 -1.1 155,411 - PS417_00740 0.25 -3.3 155,411 - PS417_00740 0.25 -0.4
Or see this region's nucleotide sequence