Experiment: JP1 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_00715 and PS417_00720 overlap by 4 nucleotides PS417_00720 and PS417_00725 overlap by 4 nucleotides PS417_00725 and PS417_00730 are separated by 81 nucleotides PS417_00730 and PS417_00735 are separated by 147 nucleotides
PS417_00715: PS417_00715 - required for octanoate transport, together with NodT, MFP, and FUSC proteins (PS417_00725, PS417_00720, PS417_00710) (from data) , at 149,595 to 149,804
_00715
PS417_00720: PS417_00720 - membrane protein, at 149,801 to 150,691
_00720
PS417_00725: PS417_00725 - RND transporter, at 150,688 to 152,130
_00725
PS417_00730: PS417_00730 - NADH:ubiquinone oxidoreductase subunit N, at 152,212 to 152,844
_00730
PS417_00735: PS417_00735 - hypothetical protein, at 152,992 to 154,488
_00735
Position (kb)
150
151
152
153 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 149.709 kb on + strand, within PS417_00715 at 149.891 kb on + strand, within PS417_00720 at 149.891 kb on + strand, within PS417_00720 at 149.891 kb on + strand at 149.891 kb on + strand, within PS417_00720 at 149.891 kb on + strand, within PS417_00720 at 149.892 kb on - strand, within PS417_00720 at 149.892 kb on - strand, within PS417_00720 at 149.892 kb on - strand, within PS417_00720 at 149.892 kb on - strand, within PS417_00720 at 149.894 kb on + strand, within PS417_00720 at 149.894 kb on + strand, within PS417_00720 at 149.894 kb on + strand, within PS417_00720 at 149.894 kb on + strand, within PS417_00720 at 149.894 kb on + strand, within PS417_00720 at 150.670 kb on + strand at 150.757 kb on + strand at 150.772 kb on - strand at 150.830 kb on + strand at 150.830 kb on + strand at 150.831 kb on - strand at 150.831 kb on - strand at 150.868 kb on + strand, within PS417_00725 at 150.868 kb on + strand, within PS417_00725 at 150.868 kb on + strand, within PS417_00725 at 150.869 kb on - strand, within PS417_00725 at 150.961 kb on + strand, within PS417_00725 at 151.375 kb on + strand, within PS417_00725 at 151.376 kb on - strand, within PS417_00725 at 151.376 kb on - strand, within PS417_00725 at 151.376 kb on - strand, within PS417_00725 at 151.865 kb on - strand, within PS417_00725 at 151.865 kb on - strand, within PS417_00725 at 152.000 kb on - strand at 152.141 kb on - strand at 152.224 kb on + strand at 152.224 kb on + strand at 152.261 kb on + strand at 152.278 kb on + strand, within PS417_00730 at 152.278 kb on + strand, within PS417_00730 at 152.278 kb on + strand, within PS417_00730 at 152.279 kb on - strand, within PS417_00730 at 152.389 kb on + strand, within PS417_00730 at 152.497 kb on + strand, within PS417_00730 at 152.512 kb on + strand, within PS417_00730 at 152.513 kb on - strand, within PS417_00730 at 152.698 kb on + strand, within PS417_00730 at 152.836 kb on + strand at 152.837 kb on - strand at 152.899 kb on + strand at 152.899 kb on + strand at 152.997 kb on + strand at 152.997 kb on + strand at 153.009 kb on - strand at 153.009 kb on - strand at 153.064 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction JP1 MOI 0.1 remove 149,709 + PS417_00715 0.54 +1.4 149,891 + PS417_00720 0.10 -0.4 149,891 + PS417_00720 0.10 -1.6 149,891 + +2.5 149,891 + PS417_00720 0.10 -1.1 149,891 + PS417_00720 0.10 +1.2 149,892 - PS417_00720 0.10 -0.4 149,892 - PS417_00720 0.10 -1.5 149,892 - PS417_00720 0.10 +0.1 149,892 - PS417_00720 0.10 -0.5 149,894 + PS417_00720 0.10 -0.5 149,894 + PS417_00720 0.10 +0.3 149,894 + PS417_00720 0.10 +0.2 149,894 + PS417_00720 0.10 +0.9 149,894 + PS417_00720 0.10 -2.3 150,670 + +0.1 150,757 + -1.8 150,772 - -0.9 150,830 + -0.6 150,830 + +0.3 150,831 - +0.1 150,831 - -0.1 150,868 + PS417_00725 0.12 +0.4 150,868 + PS417_00725 0.12 -1.1 150,868 + PS417_00725 0.12 +1.9 150,869 - PS417_00725 0.13 +0.5 150,961 + PS417_00725 0.19 +0.1 151,375 + PS417_00725 0.48 -0.3 151,376 - PS417_00725 0.48 -1.4 151,376 - PS417_00725 0.48 -1.8 151,376 - PS417_00725 0.48 -2.5 151,865 - PS417_00725 0.82 +0.3 151,865 - PS417_00725 0.82 -0.1 152,000 - -2.1 152,141 - +0.6 152,224 + -0.3 152,224 + -2.1 152,261 + -0.2 152,278 + PS417_00730 0.10 -1.0 152,278 + PS417_00730 0.10 -1.7 152,278 + PS417_00730 0.10 +0.3 152,279 - PS417_00730 0.11 -0.1 152,389 + PS417_00730 0.28 +1.4 152,497 + PS417_00730 0.45 -1.0 152,512 + PS417_00730 0.47 +1.3 152,513 - PS417_00730 0.48 -0.7 152,698 + PS417_00730 0.77 -0.5 152,836 + +0.3 152,837 - +0.4 152,899 + +0.7 152,899 + +0.1 152,997 + -0.2 152,997 + +0.1 153,009 - +0.8 153,009 - +0.6 153,064 + -1.1
Or see this region's nucleotide sequence