Strain Fitness in Pseudomonas putida KT2440 around PP_5437

Experiment: Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPP_0276 and PP_t73 are separated by 287 nucleotidesPP_t73 and PP_0277 are separated by 98 nucleotidesPP_0277 and PP_5437 are separated by 203 nucleotidesPP_5437 and PP_0278 are separated by 134 nucleotidesPP_0278 and PP_0279 are separated by 392 nucleotides PP_0276: PP_0276 - Transcriptional regulator, Cro/CI family, at 334,362 to 334,685 _0276 PP_t73: PP_t73 - tRNA-Thr, at 334,973 to 335,048 _t73 PP_0277: PP_0277 - conserved exported protein of unknown function, at 335,147 to 335,395 _0277 PP_5437: PP_5437 - protein of unknown function, at 335,599 to 335,781 _5437 PP_0278: PP_0278 - conserved protein of unknown function, at 335,916 to 336,152 _0278 PP_0279: PP_0279 - conserved protein of unknown function, at 336,545 to 337,750 _0279 Position (kb) 335 336Strain fitness (log2 ratio) -2 -1 0 1 2 3at 334.866 kb on + strandat 334.897 kb on + strandat 334.965 kb on - strandat 334.971 kb on + strandat 334.972 kb on - strandat 334.972 kb on - strandat 335.065 kb on - strandat 335.119 kb on - strandat 335.505 kb on + strandat 335.505 kb on + strandat 335.506 kb on - strandat 335.506 kb on - strandat 335.506 kb on - strandat 335.506 kb on - strandat 335.506 kb on - strandat 335.548 kb on - strandat 335.548 kb on - strandat 335.548 kb on - strandat 335.577 kb on - strandat 335.600 kb on + strandat 335.601 kb on - strandat 335.608 kb on + strandat 335.663 kb on + strand, within PP_5437at 335.664 kb on - strand, within PP_5437at 335.668 kb on + strand, within PP_5437at 335.668 kb on + strand, within PP_5437at 335.668 kb on + strand, within PP_5437at 335.669 kb on - strand, within PP_5437at 335.669 kb on - strand, within PP_5437at 335.686 kb on - strand, within PP_5437at 335.686 kb on - strand, within PP_5437at 335.765 kb on + strandat 335.765 kb on + strandat 335.765 kb on + strandat 335.765 kb on + strandat 335.765 kb on + strandat 335.766 kb on - strandat 335.766 kb on - strandat 335.790 kb on + strandat 335.790 kb on + strandat 335.790 kb on + strandat 335.791 kb on - strandat 335.825 kb on - strandat 335.850 kb on - strandat 335.998 kb on + strand, within PP_0278at 336.004 kb on + strand, within PP_0278at 336.005 kb on - strand, within PP_0278at 336.005 kb on - strand, within PP_0278at 336.033 kb on + strand, within PP_0278at 336.105 kb on - strand, within PP_0278at 336.125 kb on + strand, within PP_0278at 336.178 kb on + strandat 336.188 kb on + strandat 336.188 kb on + strandat 336.188 kb on + strandat 336.403 kb on + strandat 336.500 kb on + strandat 336.501 kb on - strandat 336.524 kb on + strandat 336.624 kb on + strandat 336.624 kb on + strandat 336.625 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs
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334,866 + +0.2
334,897 + +1.0
334,965 - -0.7
334,971 + -0.1
334,972 - -0.5
334,972 - -0.6
335,065 - -0.9
335,119 - -1.8
335,505 + -0.8
335,505 + -0.1
335,506 - -0.4
335,506 - -0.0
335,506 - -0.4
335,506 - +0.2
335,506 - +0.8
335,548 - +0.3
335,548 - +0.5
335,548 - -0.1
335,577 - -0.9
335,600 + +0.2
335,601 - +0.5
335,608 + -0.6
335,663 + PP_5437 0.35 +0.8
335,664 - PP_5437 0.36 -1.4
335,668 + PP_5437 0.38 +1.0
335,668 + PP_5437 0.38 +1.2
335,668 + PP_5437 0.38 +0.1
335,669 - PP_5437 0.38 +0.4
335,669 - PP_5437 0.38 +0.5
335,686 - PP_5437 0.48 -0.7
335,686 - PP_5437 0.48 +0.4
335,765 + -0.7
335,765 + -0.2
335,765 + -0.2
335,765 + -0.0
335,765 + +0.3
335,766 - +0.0
335,766 - -1.8
335,790 + +1.0
335,790 + -1.1
335,790 + -0.0
335,791 - -1.1
335,825 - +1.1
335,850 - -0.2
335,998 + PP_0278 0.35 +0.3
336,004 + PP_0278 0.37 +0.5
336,005 - PP_0278 0.38 -0.3
336,005 - PP_0278 0.38 +3.0
336,033 + PP_0278 0.49 -1.5
336,105 - PP_0278 0.80 +1.4
336,125 + PP_0278 0.88 -0.3
336,178 + -0.5
336,188 + -1.2
336,188 + -0.1
336,188 + +0.7
336,403 + +0.6
336,500 + -0.2
336,501 - +0.0
336,524 + +0.1
336,624 + -1.6
336,624 + +0.6
336,625 - -0.7

Or see this region's nucleotide sequence