Strain Fitness in Pseudomonas putida KT2440 around PP_5143

Experiment: Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPP_5141 and PP_5142 are separated by 51 nucleotidesPP_5142 and PP_5143 are separated by 16 nucleotidesPP_5143 and PP_5144 are separated by 130 nucleotides PP_5141: PP_5141 - Thymidylate synthase, at 5,866,734 to 5,867,705 _5141 PP_5142: PP_5142 - Prolipoprotein diacylglyceryl transferase, at 5,867,757 to 5,868,563 _5142 PP_5143: PP_5143 - conserved membrane protein of unknown function, at 5,868,580 to 5,869,362 _5143 PP_5144: PP_5144 - conserved protein of unknown function, at 5,869,493 to 5,870,239 _5144 Position (kb) 5868 5869 5870Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 5867.775 kb on - strandat 5867.787 kb on - strandat 5867.787 kb on - strandat 5867.789 kb on - strandat 5867.789 kb on - strandat 5868.726 kb on - strand, within PP_5143at 5868.732 kb on - strand, within PP_5143at 5868.732 kb on - strand, within PP_5143at 5868.732 kb on - strand, within PP_5143at 5869.347 kb on - strandat 5869.347 kb on - strandat 5869.347 kb on - strandat 5869.375 kb on - strandat 5869.395 kb on - strandat 5869.419 kb on - strandat 5869.492 kb on + strandat 5869.492 kb on + strandat 5869.492 kb on + strandat 5869.492 kb on + strandat 5869.492 kb on + strandat 5869.493 kb on - strandat 5869.493 kb on - strandat 5869.493 kb on - strandat 5869.493 kb on - strandat 5869.493 kb on - strandat 5869.493 kb on - strandat 5869.493 kb on - strandat 5869.493 kb on - strandat 5869.507 kb on - strandat 5869.569 kb on - strand, within PP_5144at 5869.784 kb on + strand, within PP_5144at 5869.784 kb on + strand, within PP_5144at 5869.785 kb on - strand, within PP_5144at 5869.817 kb on + strand, within PP_5144at 5869.818 kb on - strand, within PP_5144at 5869.863 kb on - strand, within PP_5144at 5869.936 kb on + strand, within PP_5144at 5869.936 kb on + strand, within PP_5144at 5870.123 kb on + strand, within PP_5144at 5870.241 kb on + strandat 5870.288 kb on - strandat 5870.288 kb on - strandat 5870.288 kb on - strandat 5870.319 kb on + strandat 5870.319 kb on + strandat 5870.319 kb on + strandat 5870.319 kb on + strandat 5870.320 kb on - strandat 5870.320 kb on - strandat 5870.320 kb on - strandat 5870.326 kb on + strandat 5870.327 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs
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5,867,775 - -0.4
5,867,787 - -1.5
5,867,787 - -0.5
5,867,789 - +0.1
5,867,789 - +0.0
5,868,726 - PP_5143 0.19 +1.6
5,868,732 - PP_5143 0.19 +0.1
5,868,732 - PP_5143 0.19 +0.7
5,868,732 - PP_5143 0.19 +0.0
5,869,347 - -0.3
5,869,347 - -0.3
5,869,347 - -2.1
5,869,375 - +0.3
5,869,395 - +0.2
5,869,419 - +1.0
5,869,492 + +0.9
5,869,492 + -0.0
5,869,492 + -0.6
5,869,492 + -3.2
5,869,492 + +1.2
5,869,493 - -0.8
5,869,493 - +0.3
5,869,493 - -0.5
5,869,493 - +0.6
5,869,493 - -0.5
5,869,493 - -1.2
5,869,493 - +0.4
5,869,493 - -1.1
5,869,507 - +1.8
5,869,569 - PP_5144 0.10 +0.4
5,869,784 + PP_5144 0.39 -0.8
5,869,784 + PP_5144 0.39 +0.6
5,869,785 - PP_5144 0.39 -1.5
5,869,817 + PP_5144 0.43 -0.8
5,869,818 - PP_5144 0.44 +0.5
5,869,863 - PP_5144 0.50 -0.3
5,869,936 + PP_5144 0.59 -0.0
5,869,936 + PP_5144 0.59 +0.5
5,870,123 + PP_5144 0.84 -1.1
5,870,241 + -0.3
5,870,288 - -0.8
5,870,288 - -1.8
5,870,288 - +0.1
5,870,319 + +0.3
5,870,319 + -0.0
5,870,319 + +0.5
5,870,319 + +0.9
5,870,320 - +0.2
5,870,320 - -0.2
5,870,320 - -0.5
5,870,326 + -0.7
5,870,327 - -0.3

Or see this region's nucleotide sequence