Strain Fitness in Pseudomonas putida KT2440 around PP_4422

Experiment: Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs
5,016,384	-		PP_4421	0.30	+0.8
5,016,432	+		PP_4421	0.33	+0.9
5,016,432	+		PP_4421	0.33	-0.3
5,016,433	-		PP_4421	0.33	+0.3
5,016,433	-		PP_4421	0.33	-1.1
5,016,433	-		PP_4421	0.33	+0.7
5,016,433	-		PP_4421	0.33	-0.3
5,016,434	+		PP_4421	0.33	+0.7
5,016,434	+		PP_4421	0.33	+0.6
5,016,434	+		PP_4421	0.33	-0.8
5,016,434	+		PP_4421	0.33	-0.2
5,016,434	+		PP_4421	0.33	+0.1
5,016,434	+		PP_4421	0.33	+1.1
5,016,434	+		PP_4421	0.33	+0.5
5,016,435	-		PP_4421	0.33	-0.6
5,016,435	-		PP_4421	0.33	+0.3
5,016,435	-		PP_4421	0.33	+1.2
5,016,435	-		PP_4421	0.33	-0.7
5,016,435	-		PP_4421	0.33	+0.5
5,016,435	-		PP_4421	0.33	-0.9
5,016,435	-		PP_4421	0.33	-0.8
5,016,473	+		PP_4421	0.36	+0.1
5,016,492	+		PP_4421	0.37	+0.8
5,016,492	+		PP_4421	0.37	+0.1
5,016,493	-		PP_4421	0.38	+0.5
5,016,496	+		PP_4421	0.38	-0.5
5,016,496	+		PP_4421	0.38	+0.3
5,016,497	-		PP_4421	0.38	+0.3
5,016,524	+		PP_4421	0.40	+1.1
5,016,524	+		PP_4421	0.40	-1.1
5,016,524	+		PP_4421	0.40	+0.2
5,016,525	-		PP_4421	0.40	-0.5
5,016,525	-		PP_4421	0.40	-0.8
5,016,525	-		PP_4421	0.40	+1.1
5,016,525	-		PP_4421	0.40	+0.2
5,016,525	-		PP_4421	0.40	-0.9
5,016,552	+		PP_4421	0.42	-1.3
5,016,553	-		PP_4421	0.42	-1.6
5,016,610	+		PP_4421	0.46	-0.2
5,016,610	+		PP_4421	0.46	+0.2
5,016,632	+		PP_4421	0.47	+0.2
5,016,632	+		PP_4421	0.47	-0.0
5,016,632	+		PP_4421	0.47	+0.5
5,016,633	-		PP_4421	0.48	-0.5
5,016,651	+		PP_4421	0.49	+0.1
5,016,652	-		PP_4421	0.49	+0.4
5,016,652	-		PP_4421	0.49	-0.8
5,016,654	+		PP_4421	0.49	-0.2
5,016,654	+		PP_4421	0.49	+1.0
5,016,654	+		PP_4421	0.49	-0.1
5,016,791	+		PP_4421	0.59	-0.1
5,016,791	+		PP_4421	0.59	+0.7
5,016,792	-		PP_4421	0.59	+0.7
5,016,792	-		PP_4421	0.59	-0.3
5,016,792	-		PP_4421	0.59	-0.8
5,016,795	-		PP_4421	0.59	+0.2
5,016,795	-		PP_4421	0.59	-0.1
5,016,795	-		PP_4421	0.59	+0.3
5,016,811	-		PP_4421	0.60	-0.7
5,016,851	+		PP_4421	0.63	+0.6
5,016,851	+		PP_4421	0.63	-0.8
5,016,852	-		PP_4421	0.63	-0.2
5,016,956	+		PP_4421	0.71	+0.4
5,016,956	+		PP_4421	0.71	+0.2
5,016,956	+		PP_4421	0.71	+0.5
5,016,956	+		PP_4421	0.71	+0.9
5,016,957	-		PP_4421	0.71	+0.1
5,016,957	-		PP_4421	0.71	-0.7
5,016,959	+		PP_4421	0.71	-0.2
5,016,960	-		PP_4421	0.71	-0.3
5,017,010	+		PP_4421	0.75	+0.4
5,017,010	+		PP_4421	0.75	+0.8
5,017,010	+		PP_4421	0.75	+0.0
5,017,011	-		PP_4421	0.75	+0.1
5,017,094	+		PP_4421	0.81	-0.5
5,017,094	+		PP_4421	0.81	+0.5
5,017,094	+		PP_4421	0.81	-0.1
5,017,094	+				+2.4
5,017,094	+		PP_4421	0.81	+0.4
5,017,095	-		PP_4421	0.81	-0.5
5,017,095	-		PP_4421	0.81	+0.8
5,017,095	-		PP_4421	0.81	-0.4
5,017,095	-				-0.2
5,017,095	-		PP_4421	0.81	+0.1
5,017,095	-		PP_4421	0.81	+0.3
5,017,103	+		PP_4421	0.81	+0.2
5,017,104	-		PP_4421	0.81	+1.6
5,017,104	-		PP_4421	0.81	-0.0
5,017,104	-		PP_4421	0.81	-0.2
5,017,106	+		PP_4421	0.82	+0.8
5,017,106	+		PP_4421	0.82	-0.9
5,017,106	+		PP_4421	0.82	-0.1
5,017,160	+		PP_4421	0.85	+0.1
5,017,160	+		PP_4421	0.85	+0.4
5,017,161	-		PP_4421	0.85	-0.3
5,017,178	+		PP_4421	0.87	-0.6
5,017,179	-		PP_4421	0.87	+0.2
5,017,202	+		PP_4421	0.88	+0.8
5,017,203	-		PP_4421	0.89	+0.3
5,017,227	+				+0.2
5,017,227	+				+0.6
5,017,227	+				+0.4
5,017,228	-				-0.0
5,017,228	-				+0.0
5,017,407	-				+0.1
5,017,407	-				-1.5
5,017,407	-				-2.0
5,017,421	+				-0.2
5,017,421	+				+0.4
5,017,421	+				+0.7
5,017,421	+				+0.6
5,017,549	+		PP_4422	0.11	-1.0
5,017,580	+		PP_4422	0.13	+0.9
5,017,581	-				+1.1
5,017,587	-		PP_4422	0.14	-1.5
5,017,587	-		PP_4422	0.14	-0.6
5,017,587	-		PP_4422	0.14	-1.8
5,017,587	-		PP_4422	0.14	-1.3
5,017,627	+		PP_4422	0.16	-0.1
5,017,627	+		PP_4422	0.16	+0.4
5,017,628	-		PP_4422	0.17	-1.5
5,017,889	-		PP_4422	0.34	-1.1
5,017,889	-		PP_4422	0.34	-2.1
5,017,918	+		PP_4422	0.36	-0.4
5,017,919	-		PP_4422	0.36	+0.8
5,017,931	+		PP_4422	0.37	+1.4
5,017,931	+		PP_4422	0.37	+0.6
5,017,932	-		PP_4422	0.37	-0.7
5,017,932	-		PP_4422	0.37	-0.4
5,017,949	+		PP_4422	0.38	-1.1
5,017,949	+		PP_4422	0.38	-0.6
5,017,949	+		PP_4422	0.38	-1.5
5,017,949	+		PP_4422	0.38	+0.2
5,017,949	+		PP_4422	0.38	-0.1
5,017,949	+		PP_4422	0.38	-0.2
5,017,950	-		PP_4422	0.38	-1.5
5,017,950	-		PP_4422	0.38	-0.2
5,017,950	-		PP_4422	0.38	-0.2
5,017,950	-		PP_4422	0.38	+0.0
5,017,950	-		PP_4422	0.38	-1.1
5,018,080	+				+3.1
5,018,235	+		PP_4422	0.58	-0.4
5,018,389	+		PP_4422	0.68	+0.2
5,018,390	-		PP_4422	0.68	-0.5
5,018,409	+		PP_4422	0.70	-2.3
5,018,409	+		PP_4422	0.70	-0.5
5,018,410	-		PP_4422	0.70	-0.8
5,018,410	-		PP_4422	0.70	+0.3
5,018,456	+		PP_4422	0.73	+0.3
5,018,504	+		PP_4422	0.76	-0.6
5,018,505	-		PP_4422	0.76	+1.4
5,018,505	-		PP_4422	0.76	+0.2
5,018,505	-		PP_4422	0.76	-1.1
5,018,538	+		PP_4422	0.78	-0.5
5,018,538	+		PP_4422	0.78	-1.1
5,018,539	-		PP_4422	0.78	-1.2
5,018,539	-		PP_4422	0.78	-3.6
5,018,605	-		PP_4422	0.83	-1.2
5,018,605	-		PP_4422	0.83	-1.6
5,018,605	-		PP_4422	0.83	+0.1
5,018,686	+		PP_4422	0.88	-0.6
5,018,686	+		PP_4422	0.88	-0.2
5,018,687	-		PP_4422	0.88	-0.1
5,018,687	-		PP_4422	0.88	+0.1
5,018,687	-		PP_4422	0.88	-0.2
5,018,687	-		PP_4422	0.88	-1.6
5,018,711	-				-0.3
5,018,734	-				-0.2
5,018,734	-				-0.2
5,018,734	-				+0.7
5,018,734	-				-0.2
5,018,734	-				+0.5
5,018,734	-				+0.4
5,018,734	-				-0.2
5,018,734	-				+0.0
5,018,827	-				-0.2
5,018,877	+				+1.0
5,018,878	-				-0.7
5,018,878	-				-0.0
5,018,905	+				+0.5
5,018,905	+				-1.0
5,018,905	+				-0.7
5,018,905	+				-0.3
5,018,906	-				-0.8
5,018,906	-				-0.8
5,018,906	-				+0.5
5,018,906	-				+1.0
5,018,906	-				-0.8
5,018,906	-				-0.2
5,018,906	-				-0.5
5,019,026	-				-0.4
5,019,026	-				-0.5
5,019,033	+				+0.2
5,019,034	-				-0.8
5,019,117	+				+0.7
5,019,117	+				+0.1
5,019,214	-				-0.2
5,019,220	+		PP_4423	0.10	+0.3
5,019,221	-		PP_4423	0.11	-0.7
5,019,249	+		PP_4423	0.13	+0.1
5,019,303	+		PP_4423	0.19	-0.2
5,019,303	+		PP_4423	0.19	+1.2
5,019,303	+		PP_4423	0.19	+0.0
5,019,303	+		PP_4423	0.19	-1.6
5,019,383	-		PP_4423	0.27	+0.4
5,019,491	-		PP_4423	0.38	+0.3
5,019,527	-		PP_4423	0.41	-0.2
5,019,570	+		PP_4423	0.46	+0.4
5,019,634	+		PP_4423	0.52	-0.3
5,019,729	+		PP_4423	0.62	-0.9
5,019,779	-		PP_4423	0.67	-0.3