Strain Fitness in Pseudomonas simiae WCS417 around PS417_04355

Experiment: JP1 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPS417_04345 and PS417_04350 are separated by 185 nucleotidesPS417_04350 and PS417_04355 overlap by 1 nucleotidesPS417_04355 and PS417_04360 overlap by 14 nucleotidesPS417_04360 and PS417_04365 are separated by 8 nucleotides PS417_04345: PS417_04345 - RNA polymerase factor sigma-54, at 971,057 to 972,550 _04345 PS417_04350: PS417_04350 - sugar ABC transporter ATP-binding protein, at 972,736 to 973,461 _04350 PS417_04355: PS417_04355 - organic solvent tolerance protein OstA, at 973,461 to 973,994 _04355 PS417_04360: PS417_04360 - lipopolysaccharide ABC transporter permease, at 973,981 to 974,553 _04360 PS417_04365: PS417_04365 - 3-deoxy-D-manno-octulosonate 8-phosphate phosphatase, at 974,562 to 975,086 _04365 Position (kb) 973 974Strain fitness (log2 ratio) -2 -1 0 1at 972.657 kb on - strandat 972.738 kb on - strandat 972.738 kb on - strandat 974.564 kb on - strandat 974.566 kb on - strandat 974.566 kb on - strandat 974.566 kb on - strandat 974.780 kb on - strand, within PS417_04365at 974.785 kb on - strand, within PS417_04365

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Per-strain Table

Position Strand Gene LocusTag Fraction JP1 MOI 1
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972,657 - +0.2
972,738 - -0.9
972,738 - -0.7
974,564 - -2.1
974,566 - -2.6
974,566 - -0.4
974,566 - -1.3
974,780 - PS417_04365 0.42 -0.6
974,785 - PS417_04365 0.42 +0.9

Or see this region's nucleotide sequence