Experiment: JP1 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_03620 and PS417_03625 are separated by 112 nucleotides PS417_03625 and PS417_03630 are separated by 136 nucleotides PS417_03630 and PS417_03635 are separated by 137 nucleotides PS417_03635 and PS417_03640 overlap by 8 nucleotides
PS417_03620: PS417_03620 - glutamate racemase, at 822,680 to 823,474
_03620
PS417_03625: PS417_03625 - lipid A 3-O-deacylase, at 823,587 to 824,105
_03625
PS417_03630: PS417_03630 - hypothetical protein, at 824,242 to 824,463
_03630
PS417_03635: PS417_03635 - membrane protein, at 824,601 to 825,080
_03635
PS417_03640: PS417_03640 - cyclopropane-fatty-acyl-phospholipid synthase, at 825,073 to 826,344
_03640
Position (kb) 824
825 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 823.435 kb on - strand at 823.531 kb on + strand at 823.531 kb on + strand at 823.532 kb on - strand at 823.532 kb on - strand at 823.541 kb on + strand at 823.549 kb on - strand at 823.549 kb on - strand at 823.560 kb on + strand at 823.560 kb on + strand at 823.560 kb on + strand at 823.560 kb on + strand at 823.734 kb on - strand, within PS417_03625 at 823.795 kb on - strand, within PS417_03625 at 823.795 kb on - strand, within PS417_03625 at 823.884 kb on + strand, within PS417_03625 at 823.884 kb on + strand, within PS417_03625 at 823.884 kb on + strand, within PS417_03625 at 823.885 kb on - strand, within PS417_03625 at 823.885 kb on - strand, within PS417_03625 at 823.885 kb on - strand, within PS417_03625 at 823.886 kb on + strand, within PS417_03625 at 823.886 kb on + strand, within PS417_03625 at 823.886 kb on + strand, within PS417_03625 at 824.076 kb on + strand at 824.076 kb on + strand at 824.077 kb on - strand at 824.088 kb on + strand at 824.089 kb on - strand at 824.089 kb on - strand at 824.104 kb on - strand at 824.170 kb on + strand at 824.185 kb on + strand at 824.186 kb on - strand at 824.221 kb on - strand at 824.340 kb on + strand, within PS417_03630 at 824.340 kb on + strand, within PS417_03630 at 824.341 kb on - strand, within PS417_03630 at 824.341 kb on - strand, within PS417_03630 at 824.428 kb on - strand, within PS417_03630 at 824.429 kb on + strand, within PS417_03630 at 824.560 kb on - strand at 824.560 kb on - strand at 824.575 kb on + strand at 824.576 kb on - strand at 824.625 kb on + strand at 824.625 kb on + strand at 824.911 kb on - strand, within PS417_03635 at 824.959 kb on - strand, within PS417_03635 at 825.075 kb on - strand at 825.185 kb on + strand at 825.209 kb on + strand, within PS417_03640 at 825.210 kb on - strand, within PS417_03640 at 825.210 kb on - strand, within PS417_03640 at 825.390 kb on + strand, within PS417_03640 at 825.391 kb on - strand, within PS417_03640 at 825.392 kb on + strand, within PS417_03640 at 825.393 kb on - strand, within PS417_03640
Per-strain Table
Position Strand Gene LocusTag Fraction JP1 MOI 1 remove 823,435 - +0.5 823,531 + +2.4 823,531 + +0.0 823,532 - -0.1 823,532 - +1.8 823,541 + -1.2 823,549 - -0.2 823,549 - -0.7 823,560 + -0.2 823,560 + +3.8 823,560 + -2.0 823,560 + +0.7 823,734 - PS417_03625 0.28 +0.1 823,795 - PS417_03625 0.40 +0.2 823,795 - PS417_03625 0.40 +0.3 823,884 + PS417_03625 0.57 -0.2 823,884 + PS417_03625 0.57 +0.4 823,884 + PS417_03625 0.57 +0.5 823,885 - PS417_03625 0.57 -0.6 823,885 - PS417_03625 0.57 -1.5 823,885 - PS417_03625 0.57 +0.2 823,886 + PS417_03625 0.58 -0.4 823,886 + PS417_03625 0.58 +2.1 823,886 + PS417_03625 0.58 +0.3 824,076 + +0.8 824,076 + +0.4 824,077 - +0.6 824,088 + -0.2 824,089 - -3.4 824,089 - -0.2 824,104 - -1.9 824,170 + +0.7 824,185 + +0.3 824,186 - +1.1 824,221 - +0.9 824,340 + PS417_03630 0.44 -2.6 824,340 + PS417_03630 0.44 -0.8 824,341 - PS417_03630 0.45 +0.4 824,341 - PS417_03630 0.45 -1.1 824,428 - PS417_03630 0.84 -1.4 824,429 + PS417_03630 0.84 -2.3 824,560 - +0.1 824,560 - -0.7 824,575 + +0.3 824,576 - +1.1 824,625 + +1.0 824,625 + -1.4 824,911 - PS417_03635 0.65 +0.4 824,959 - PS417_03635 0.75 -1.6 825,075 - +0.6 825,185 + -1.3 825,209 + PS417_03640 0.11 +0.6 825,210 - PS417_03640 0.11 -1.5 825,210 - PS417_03640 0.11 +2.9 825,390 + PS417_03640 0.25 -1.7 825,391 - PS417_03640 0.25 +0.1 825,392 + PS417_03640 0.25 -0.4 825,393 - PS417_03640 0.25 +1.1
Or see this region's nucleotide sequence