Experiment: JP1 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_03485 and PS417_03490 overlap by 4 nucleotides PS417_03490 and PS417_03495 are separated by 7 nucleotides PS417_03495 and PS417_03500 are separated by 10 nucleotides PS417_03500 and PS417_03505 overlap by 4 nucleotides
PS417_03485: PS417_03485 - type III secretion protein, at 800,283 to 801,296
_03485
PS417_03490: PS417_03490 - type III secretion system protein SsaR, at 801,293 to 801,946
_03490
PS417_03495: PS417_03495 - type III secretory protein EscS, at 801,954 to 802,217
_03495
PS417_03500: PS417_03500 - type III secretion system protein, at 802,228 to 803,016
_03500
PS417_03505: PS417_03505 - type III secretion system protein, at 803,013 to 804,110
_03505
Position (kb) 801
802
803 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 800.994 kb on - strand, within PS417_03485 at 801.058 kb on + strand, within PS417_03485 at 801.103 kb on + strand, within PS417_03485 at 801.104 kb on - strand, within PS417_03485 at 801.104 kb on - strand, within PS417_03485 at 801.104 kb on - strand, within PS417_03485 at 801.126 kb on - strand, within PS417_03485 at 801.204 kb on + strand at 801.204 kb on + strand at 801.204 kb on + strand at 801.247 kb on + strand at 801.636 kb on + strand, within PS417_03490 at 801.636 kb on + strand, within PS417_03490 at 801.637 kb on - strand, within PS417_03490 at 801.637 kb on - strand, within PS417_03490 at 801.780 kb on - strand, within PS417_03490 at 801.780 kb on - strand, within PS417_03490 at 801.938 kb on + strand at 802.075 kb on + strand, within PS417_03495 at 802.076 kb on - strand, within PS417_03495 at 802.076 kb on - strand, within PS417_03495 at 802.237 kb on + strand at 802.278 kb on + strand at 802.279 kb on - strand at 802.323 kb on + strand, within PS417_03500 at 802.323 kb on + strand, within PS417_03500 at 802.323 kb on + strand, within PS417_03500 at 802.323 kb on + strand, within PS417_03500 at 802.324 kb on - strand, within PS417_03500 at 802.325 kb on + strand, within PS417_03500 at 802.325 kb on + strand, within PS417_03500 at 802.325 kb on + strand, within PS417_03500 at 802.325 kb on + strand, within PS417_03500 at 802.325 kb on + strand, within PS417_03500 at 802.325 kb on + strand, within PS417_03500 at 802.326 kb on - strand, within PS417_03500 at 802.326 kb on - strand, within PS417_03500 at 802.326 kb on - strand, within PS417_03500 at 802.326 kb on - strand, within PS417_03500 at 802.636 kb on - strand, within PS417_03500 at 802.678 kb on + strand, within PS417_03500 at 802.844 kb on - strand, within PS417_03500 at 802.918 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction JP1 MOI 1 remove 800,994 - PS417_03485 0.70 +0.7 801,058 + PS417_03485 0.76 -1.9 801,103 + PS417_03485 0.81 +0.3 801,104 - PS417_03485 0.81 -1.7 801,104 - PS417_03485 0.81 -1.7 801,104 - PS417_03485 0.81 +0.9 801,126 - PS417_03485 0.83 +1.1 801,204 + +0.3 801,204 + +0.0 801,204 + -1.0 801,247 + -0.2 801,636 + PS417_03490 0.52 +0.0 801,636 + PS417_03490 0.52 +1.6 801,637 - PS417_03490 0.53 -0.7 801,637 - PS417_03490 0.53 +0.4 801,780 - PS417_03490 0.74 -0.8 801,780 - PS417_03490 0.74 +3.6 801,938 + -0.2 802,075 + PS417_03495 0.46 -0.1 802,076 - PS417_03495 0.46 -1.2 802,076 - PS417_03495 0.46 -0.9 802,237 + -3.4 802,278 + +0.4 802,279 - +0.3 802,323 + PS417_03500 0.12 -0.5 802,323 + PS417_03500 0.12 -0.2 802,323 + PS417_03500 0.12 +2.4 802,323 + PS417_03500 0.12 -2.8 802,324 - PS417_03500 0.12 +0.5 802,325 + PS417_03500 0.12 +0.8 802,325 + PS417_03500 0.12 -1.4 802,325 + PS417_03500 0.12 +0.1 802,325 + PS417_03500 0.12 +1.4 802,325 + PS417_03500 0.12 +0.2 802,325 + PS417_03500 0.12 -1.3 802,326 - PS417_03500 0.12 -0.3 802,326 - PS417_03500 0.12 +0.1 802,326 - PS417_03500 0.12 -0.1 802,326 - PS417_03500 0.12 +1.1 802,636 - PS417_03500 0.52 -0.3 802,678 + PS417_03500 0.57 +1.1 802,844 - PS417_03500 0.78 +0.2 802,918 + +3.8
Or see this region's nucleotide sequence