Experiment: JP1 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_03400 and PS417_03405 overlap by 11 nucleotides PS417_03405 and PS417_03410 are separated by 25 nucleotides
PS417_03400: PS417_03400 - D-alanine--D-alanine ligase, at 779,984 to 781,078
_03400
PS417_03405: PS417_03405 - D-alanyl-alanine synthetase, at 781,068 to 781,793
_03405
PS417_03410: PS417_03410 - gamma-glutamyltransferase, at 781,819 to 783,630
_03410
Position (kb) 781
782 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 780.472 kb on + strand, within PS417_03400 at 780.488 kb on - strand, within PS417_03400 at 780.488 kb on - strand, within PS417_03400 at 780.488 kb on - strand, within PS417_03400 at 780.488 kb on - strand, within PS417_03400 at 780.594 kb on - strand, within PS417_03400 at 780.743 kb on + strand, within PS417_03400 at 780.762 kb on - strand, within PS417_03400 at 780.966 kb on - strand, within PS417_03400 at 780.999 kb on - strand at 781.096 kb on + strand at 781.168 kb on + strand, within PS417_03405 at 781.168 kb on + strand, within PS417_03405 at 781.168 kb on + strand, within PS417_03405 at 781.405 kb on + strand, within PS417_03405 at 781.405 kb on + strand, within PS417_03405 at 781.406 kb on - strand, within PS417_03405 at 781.406 kb on - strand, within PS417_03405 at 781.494 kb on + strand, within PS417_03405 at 781.494 kb on + strand, within PS417_03405 at 781.495 kb on - strand at 781.620 kb on - strand, within PS417_03405 at 781.621 kb on + strand, within PS417_03405 at 781.621 kb on + strand at 781.621 kb on + strand, within PS417_03405 at 781.622 kb on - strand, within PS417_03405 at 781.622 kb on - strand, within PS417_03405 at 781.622 kb on - strand, within PS417_03405 at 781.622 kb on - strand, within PS417_03405 at 781.622 kb on - strand, within PS417_03405 at 781.622 kb on - strand, within PS417_03405 at 781.623 kb on + strand, within PS417_03405 at 781.624 kb on - strand, within PS417_03405 at 781.624 kb on - strand, within PS417_03405 at 781.624 kb on - strand, within PS417_03405 at 781.778 kb on + strand at 781.817 kb on - strand at 782.099 kb on + strand, within PS417_03410 at 782.100 kb on - strand, within PS417_03410 at 782.415 kb on - strand, within PS417_03410
Per-strain Table
Position Strand Gene LocusTag Fraction JP1 MOI 1 remove 780,472 + PS417_03400 0.45 -0.1 780,488 - PS417_03400 0.46 -2.4 780,488 - PS417_03400 0.46 +0.2 780,488 - PS417_03400 0.46 -0.6 780,488 - PS417_03400 0.46 +0.3 780,594 - PS417_03400 0.56 -0.8 780,743 + PS417_03400 0.69 -2.0 780,762 - PS417_03400 0.71 +1.0 780,966 - PS417_03400 0.90 -2.0 780,999 - +0.3 781,096 + -0.5 781,168 + PS417_03405 0.14 -0.9 781,168 + PS417_03405 0.14 -2.3 781,168 + PS417_03405 0.14 -0.4 781,405 + PS417_03405 0.46 -0.2 781,405 + PS417_03405 0.46 -2.4 781,406 - PS417_03405 0.47 -1.7 781,406 - PS417_03405 0.47 -1.2 781,494 + PS417_03405 0.59 -1.1 781,494 + PS417_03405 0.59 -0.2 781,495 - +0.4 781,620 - PS417_03405 0.76 +1.6 781,621 + PS417_03405 0.76 -0.2 781,621 + +1.4 781,621 + PS417_03405 0.76 -0.1 781,622 - PS417_03405 0.76 -0.7 781,622 - PS417_03405 0.76 -3.2 781,622 - PS417_03405 0.76 +0.1 781,622 - PS417_03405 0.76 -2.5 781,622 - PS417_03405 0.76 +0.9 781,622 - PS417_03405 0.76 -2.0 781,623 + PS417_03405 0.76 -0.9 781,624 - PS417_03405 0.77 -1.5 781,624 - PS417_03405 0.77 +0.4 781,624 - PS417_03405 0.77 -0.0 781,778 + -2.6 781,817 - +1.3 782,099 + PS417_03410 0.15 +0.6 782,100 - PS417_03410 0.16 -3.2 782,415 - PS417_03410 0.33 -1.5
Or see this region's nucleotide sequence