Experiment: JP1 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_03210 and PS417_03215 are separated by 0 nucleotides PS417_03215 and PS417_03220 are separated by 184 nucleotides PS417_03220 and PS417_03225 are separated by 35 nucleotides
PS417_03210: PS417_03210 - calcium:proton antiporter, at 733,815 to 734,906
_03210
PS417_03215: PS417_03215 - 8-oxoguanine deaminase, at 734,907 to 736,262
_03215
PS417_03220: PS417_03220 - short-chain dehydrogenase, at 736,447 to 737,133
_03220
PS417_03225: PS417_03225 - thymidylate synthase, at 737,169 to 737,750
_03225
Position (kb) 734
735
736
737 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 734.124 kb on + strand, within PS417_03210 at 734.125 kb on - strand, within PS417_03210 at 734.230 kb on - strand, within PS417_03210 at 734.343 kb on + strand, within PS417_03210 at 734.343 kb on + strand, within PS417_03210 at 734.343 kb on + strand, within PS417_03210 at 734.343 kb on + strand at 734.343 kb on + strand, within PS417_03210 at 734.343 kb on + strand, within PS417_03210 at 734.344 kb on - strand, within PS417_03210 at 734.344 kb on - strand, within PS417_03210 at 734.344 kb on - strand, within PS417_03210 at 734.344 kb on - strand, within PS417_03210 at 734.344 kb on - strand, within PS417_03210 at 734.382 kb on + strand, within PS417_03210 at 734.382 kb on + strand, within PS417_03210 at 734.394 kb on + strand, within PS417_03210 at 734.667 kb on - strand, within PS417_03210 at 734.667 kb on - strand, within PS417_03210 at 734.667 kb on - strand, within PS417_03210 at 734.880 kb on - strand at 734.905 kb on - strand at 735.192 kb on + strand, within PS417_03215 at 735.241 kb on + strand, within PS417_03215 at 735.241 kb on + strand, within PS417_03215 at 735.242 kb on - strand, within PS417_03215 at 735.242 kb on - strand, within PS417_03215 at 735.496 kb on + strand, within PS417_03215 at 735.496 kb on + strand, within PS417_03215 at 735.497 kb on - strand, within PS417_03215 at 735.497 kb on - strand, within PS417_03215 at 735.691 kb on + strand, within PS417_03215 at 735.692 kb on - strand, within PS417_03215 at 735.837 kb on + strand, within PS417_03215 at 735.838 kb on - strand, within PS417_03215 at 735.843 kb on + strand, within PS417_03215 at 735.880 kb on + strand, within PS417_03215 at 735.880 kb on + strand, within PS417_03215 at 735.881 kb on - strand, within PS417_03215 at 736.055 kb on - strand, within PS417_03215 at 736.055 kb on - strand, within PS417_03215 at 736.055 kb on - strand, within PS417_03215 at 736.055 kb on - strand, within PS417_03215 at 736.358 kb on + strand at 736.358 kb on + strand at 736.358 kb on + strand at 736.358 kb on + strand at 736.364 kb on + strand at 736.364 kb on + strand at 736.364 kb on + strand at 736.381 kb on + strand at 736.381 kb on + strand at 736.382 kb on - strand at 736.388 kb on - strand at 736.432 kb on - strand at 736.448 kb on + strand at 736.505 kb on + strand at 736.506 kb on - strand at 736.685 kb on + strand, within PS417_03220 at 736.686 kb on - strand at 737.179 kb on - strand at 737.231 kb on + strand, within PS417_03225 at 737.231 kb on + strand, within PS417_03225 at 737.232 kb on - strand, within PS417_03225 at 737.232 kb on - strand, within PS417_03225 at 737.232 kb on - strand, within PS417_03225 at 737.232 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction JP1 MOI 1 remove 734,124 + PS417_03210 0.28 +0.6 734,125 - PS417_03210 0.28 -1.5 734,230 - PS417_03210 0.38 +0.1 734,343 + PS417_03210 0.48 -0.1 734,343 + PS417_03210 0.48 -3.5 734,343 + PS417_03210 0.48 +0.1 734,343 + +1.5 734,343 + PS417_03210 0.48 +1.8 734,343 + PS417_03210 0.48 +0.1 734,344 - PS417_03210 0.48 +0.6 734,344 - PS417_03210 0.48 +0.5 734,344 - PS417_03210 0.48 -0.5 734,344 - PS417_03210 0.48 -0.4 734,344 - PS417_03210 0.48 -0.5 734,382 + PS417_03210 0.52 +0.4 734,382 + PS417_03210 0.52 +0.0 734,394 + PS417_03210 0.53 +0.6 734,667 - PS417_03210 0.78 -0.5 734,667 - PS417_03210 0.78 +0.1 734,667 - PS417_03210 0.78 -0.6 734,880 - +0.1 734,905 - +0.6 735,192 + PS417_03215 0.21 +0.0 735,241 + PS417_03215 0.25 +0.3 735,241 + PS417_03215 0.25 -0.2 735,242 - PS417_03215 0.25 -3.1 735,242 - PS417_03215 0.25 +1.5 735,496 + PS417_03215 0.43 -1.8 735,496 + PS417_03215 0.43 -2.0 735,497 - PS417_03215 0.44 -0.2 735,497 - PS417_03215 0.44 +0.2 735,691 + PS417_03215 0.58 -0.5 735,692 - PS417_03215 0.58 -0.2 735,837 + PS417_03215 0.69 -1.3 735,838 - PS417_03215 0.69 -0.2 735,843 + PS417_03215 0.69 -1.5 735,880 + PS417_03215 0.72 -2.9 735,880 + PS417_03215 0.72 +0.5 735,881 - PS417_03215 0.72 -1.5 736,055 - PS417_03215 0.85 +0.1 736,055 - PS417_03215 0.85 +0.3 736,055 - PS417_03215 0.85 -1.5 736,055 - PS417_03215 0.85 +0.4 736,358 + -0.3 736,358 + +0.4 736,358 + +0.3 736,358 + +0.1 736,364 + +0.3 736,364 + -0.6 736,364 + +1.9 736,381 + -0.1 736,381 + -2.1 736,382 - -2.9 736,388 - -0.3 736,432 - +0.5 736,448 + -2.7 736,505 + -1.9 736,506 - -0.0 736,685 + PS417_03220 0.35 -2.9 736,686 - +0.5 737,179 - +0.2 737,231 + PS417_03225 0.11 +0.4 737,231 + PS417_03225 0.11 +1.5 737,232 - PS417_03225 0.11 +0.4 737,232 - PS417_03225 0.11 +0.3 737,232 - PS417_03225 0.11 -0.9 737,232 - -0.5
Or see this region's nucleotide sequence