Experiment: JP1 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_02810 and PS417_02815 are separated by 31 nucleotides PS417_02815 and PS417_02820 are separated by 12 nucleotides PS417_02820 and PS417_02825 are separated by 8 nucleotides
PS417_02810: PS417_02810 - urease accessory protein UreD, at 636,914 to 637,753
_02810
PS417_02815: PS417_02815 - urea ABC transporter ATP-binding protein, at 637,785 to 638,483
_02815
PS417_02820: PS417_02820 - urea ABC transporter ATP-binding protein, at 638,496 to 639,317
_02820
PS417_02825: PS417_02825 - amino acid ABC transporter permease, at 639,326 to 640,393
_02825
Position (kb) 637
638
639 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 636.814 kb on + strand at 636.814 kb on + strand at 636.815 kb on - strand at 636.815 kb on - strand at 637.806 kb on + strand at 637.806 kb on + strand at 637.806 kb on + strand at 637.806 kb on + strand at 637.806 kb on + strand at 637.874 kb on - strand, within PS417_02815 at 637.903 kb on + strand, within PS417_02815 at 637.904 kb on - strand, within PS417_02815 at 638.134 kb on + strand, within PS417_02815 at 638.456 kb on - strand at 638.509 kb on + strand at 638.877 kb on + strand, within PS417_02820 at 638.981 kb on + strand, within PS417_02820 at 639.157 kb on + strand, within PS417_02820 at 639.363 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction JP1 MOI 1 remove 636,814 + +0.4 636,814 + +3.2 636,815 - -0.5 636,815 - +1.5 637,806 + +0.5 637,806 + +2.5 637,806 + -0.1 637,806 + -0.4 637,806 + -2.5 637,874 - PS417_02815 0.13 -0.9 637,903 + PS417_02815 0.17 -2.8 637,904 - PS417_02815 0.17 -1.3 638,134 + PS417_02815 0.50 -1.5 638,456 - +0.7 638,509 + +0.3 638,877 + PS417_02820 0.46 -1.8 638,981 + PS417_02820 0.59 -2.5 639,157 + PS417_02820 0.80 -0.1 639,363 + -1.7
Or see this region's nucleotide sequence