Experiment: JP1 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_02795 and PS417_02800 are separated by 5 nucleotides PS417_02800 and PS417_02805 are separated by 9 nucleotides PS417_02805 and PS417_02810 are separated by 161 nucleotides PS417_02810 and PS417_02815 are separated by 31 nucleotides PS417_02815 and PS417_02820 are separated by 12 nucleotides
PS417_02795: PS417_02795 - acetyltransferase, at 635,389 to 635,922
_02795
PS417_02800: PS417_02800 - acetyltransferase, at 635,928 to 636,440
_02800
PS417_02805: PS417_02805 - urease subunit gamma, at 636,450 to 636,752
_02805
PS417_02810: PS417_02810 - urease accessory protein UreD, at 636,914 to 637,753
_02810
PS417_02815: PS417_02815 - urea ABC transporter ATP-binding protein, at 637,785 to 638,483
_02815
PS417_02820: PS417_02820 - urea ABC transporter ATP-binding protein, at 638,496 to 639,317
_02820
Position (kb) 636
637
638 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 636.188 kb on - strand, within PS417_02800 at 636.640 kb on + strand, within PS417_02805 at 636.641 kb on - strand, within PS417_02805 at 636.658 kb on + strand, within PS417_02805 at 636.782 kb on + strand at 636.814 kb on + strand at 636.814 kb on + strand at 636.815 kb on - strand at 636.815 kb on - strand at 637.806 kb on + strand at 637.806 kb on + strand at 637.806 kb on + strand at 637.806 kb on + strand at 637.806 kb on + strand at 637.874 kb on - strand, within PS417_02815 at 637.903 kb on + strand, within PS417_02815 at 637.904 kb on - strand, within PS417_02815 at 638.134 kb on + strand, within PS417_02815 at 638.456 kb on - strand at 638.509 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction JP1 MOI 1 remove 636,188 - PS417_02800 0.51 +0.6 636,640 + PS417_02805 0.63 +1.5 636,641 - PS417_02805 0.63 +0.4 636,658 + PS417_02805 0.69 +1.7 636,782 + -0.1 636,814 + +0.4 636,814 + +3.2 636,815 - -0.5 636,815 - +1.5 637,806 + +0.5 637,806 + +2.5 637,806 + -0.1 637,806 + -0.4 637,806 + -2.5 637,874 - PS417_02815 0.13 -0.9 637,903 + PS417_02815 0.17 -2.8 637,904 - PS417_02815 0.17 -1.3 638,134 + PS417_02815 0.50 -1.5 638,456 - +0.7 638,509 + +0.3
Or see this region's nucleotide sequence