Experiment: JP1 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_26965 and PS417_26970 are separated by 51 nucleotides PS417_26970 and PS417_26975 are separated by 78 nucleotides PS417_26975 and PS417_26980 are separated by 120 nucleotides PS417_26980 and PS417_26985 are separated by 104 nucleotides
PS417_26965: PS417_26965 - thymidylate synthase, at 5,824,294 to 5,825,127
_26965
PS417_26970: PS417_26970 - prolipoprotein diacylglyceryl transferase, at 5,825,179 to 5,825,991
_26970
PS417_26975: PS417_26975 - membrane protein, at 5,826,070 to 5,826,852
_26975
PS417_26980: PS417_26980 - hypothetical protein, at 5,826,973 to 5,827,719
_26980
PS417_26985: PS417_26985 - phosphoenolpyruvate-protein phosphotransferase, at 5,827,824 to 5,830,103
_26985
Position (kb) 5826
5827 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 5825.181 kb on - strand at 5826.006 kb on - strand at 5826.006 kb on - strand at 5826.006 kb on - strand at 5826.048 kb on - strand at 5826.059 kb on - strand at 5826.215 kb on + strand, within PS417_26975 at 5826.215 kb on + strand, within PS417_26975 at 5826.216 kb on - strand, within PS417_26975 at 5826.226 kb on + strand, within PS417_26975 at 5826.226 kb on + strand, within PS417_26975 at 5826.231 kb on + strand, within PS417_26975 at 5826.232 kb on - strand, within PS417_26975 at 5826.232 kb on - strand, within PS417_26975 at 5826.232 kb on - strand, within PS417_26975 at 5826.277 kb on + strand, within PS417_26975 at 5826.278 kb on - strand, within PS417_26975 at 5826.656 kb on - strand, within PS417_26975 at 5826.672 kb on + strand, within PS417_26975 at 5826.808 kb on + strand at 5826.836 kb on + strand at 5826.836 kb on + strand at 5826.837 kb on - strand at 5826.941 kb on - strand at 5827.048 kb on + strand, within PS417_26980 at 5827.100 kb on - strand, within PS417_26980 at 5827.265 kb on - strand, within PS417_26980 at 5827.297 kb on + strand, within PS417_26980 at 5827.345 kb on + strand, within PS417_26980 at 5827.345 kb on + strand, within PS417_26980 at 5827.345 kb on + strand at 5827.345 kb on + strand, within PS417_26980 at 5827.346 kb on - strand, within PS417_26980 at 5827.346 kb on - strand, within PS417_26980 at 5827.703 kb on + strand at 5827.704 kb on - strand at 5827.717 kb on + strand at 5827.736 kb on + strand at 5827.736 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction JP1 MOI 1 remove 5,825,181 - +0.4 5,826,006 - -1.2 5,826,006 - +1.0 5,826,006 - +0.3 5,826,048 - +0.8 5,826,059 - -1.3 5,826,215 + PS417_26975 0.19 -0.1 5,826,215 + PS417_26975 0.19 +0.9 5,826,216 - PS417_26975 0.19 +1.2 5,826,226 + PS417_26975 0.20 -1.3 5,826,226 + PS417_26975 0.20 -0.7 5,826,231 + PS417_26975 0.21 +1.7 5,826,232 - PS417_26975 0.21 +2.5 5,826,232 - PS417_26975 0.21 +2.1 5,826,232 - PS417_26975 0.21 -0.1 5,826,277 + PS417_26975 0.26 +0.2 5,826,278 - PS417_26975 0.27 +2.9 5,826,656 - PS417_26975 0.75 +1.9 5,826,672 + PS417_26975 0.77 +0.6 5,826,808 + +1.6 5,826,836 + +1.3 5,826,836 + -2.1 5,826,837 - +1.1 5,826,941 - -1.0 5,827,048 + PS417_26980 0.10 -0.2 5,827,100 - PS417_26980 0.17 +0.8 5,827,265 - PS417_26980 0.39 +0.4 5,827,297 + PS417_26980 0.43 +0.2 5,827,345 + PS417_26980 0.50 +3.1 5,827,345 + PS417_26980 0.50 +2.2 5,827,345 + -0.1 5,827,345 + PS417_26980 0.50 -0.1 5,827,346 - PS417_26980 0.50 -1.9 5,827,346 - PS417_26980 0.50 -2.2 5,827,703 + -3.9 5,827,704 - -0.0 5,827,717 + +0.6 5,827,736 + -1.8 5,827,736 + -0.5
Or see this region's nucleotide sequence