Experiment: JP1 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_26675 and PS417_26680 are separated by 77 nucleotides PS417_26680 and PS417_26685 are separated by 67 nucleotides PS417_26685 and PS417_26690 overlap by 1 nucleotides
PS417_26675: PS417_26675 - glutathione synthetase, at 5,767,215 to 5,768,168
_26675
PS417_26680: PS417_26680 - energy transducer TonB, at 5,768,246 to 5,769,151
_26680
PS417_26685: PS417_26685 - hypothetical protein, at 5,769,219 to 5,769,788
_26685
PS417_26690: PS417_26690 - Holliday junction resolvase, at 5,769,788 to 5,770,225
_26690
Position (kb) 5768
5769
5770 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 5768.169 kb on + strand at 5768.171 kb on + strand at 5768.171 kb on + strand at 5768.171 kb on + strand at 5768.171 kb on + strand at 5768.171 kb on + strand at 5768.172 kb on - strand at 5768.172 kb on - strand at 5768.172 kb on - strand at 5768.172 kb on - strand at 5769.309 kb on + strand, within PS417_26685 at 5769.444 kb on + strand, within PS417_26685 at 5769.580 kb on + strand, within PS417_26685
Per-strain Table
Position Strand Gene LocusTag Fraction JP1 MOI 1 remove 5,768,169 + +0.4 5,768,171 + -0.1 5,768,171 + +1.5 5,768,171 + +1.0 5,768,171 + -0.8 5,768,171 + -1.1 5,768,172 - -0.2 5,768,172 - -2.9 5,768,172 - -1.0 5,768,172 - +0.4 5,769,309 + PS417_26685 0.16 +0.5 5,769,444 + PS417_26685 0.39 +0.9 5,769,580 + PS417_26685 0.63 -1.2
Or see this region's nucleotide sequence