Experiment: JP1 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_25225 and PS417_25230 overlap by 4 nucleotides PS417_25230 and PS417_25235 are separated by 150 nucleotides PS417_25235 and PS417_25240 are separated by 66 nucleotides
PS417_25225: PS417_25225 - MFS transporter, at 5,467,431 to 5,467,850
_25225
PS417_25230: PS417_25230 - GTP cyclohydrolase, at 5,467,847 to 5,468,464
_25230
PS417_25235: PS417_25235 - ABC transporter substrate-binding protein, at 5,468,615 to 5,469,334
_25235
PS417_25240: PS417_25240 - thiamine monophosphate kinase, at 5,469,401 to 5,470,366
_25240
Position (kb)
5468
5469
5470 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 5467.658 kb on + strand, within PS417_25225 at 5467.658 kb on + strand, within PS417_25225 at 5467.802 kb on - strand, within PS417_25225 at 5468.505 kb on + strand at 5468.506 kb on - strand at 5468.548 kb on + strand at 5468.587 kb on - strand at 5468.587 kb on - strand at 5468.591 kb on - strand at 5468.616 kb on + strand at 5468.616 kb on + strand at 5468.617 kb on - strand at 5468.617 kb on - strand at 5468.617 kb on - strand at 5468.700 kb on - strand at 5468.700 kb on - strand, within PS417_25235 at 5468.745 kb on + strand, within PS417_25235 at 5468.745 kb on + strand, within PS417_25235 at 5468.745 kb on + strand, within PS417_25235 at 5468.745 kb on + strand, within PS417_25235 at 5468.745 kb on + strand, within PS417_25235 at 5468.746 kb on - strand, within PS417_25235 at 5468.811 kb on + strand, within PS417_25235 at 5468.812 kb on - strand, within PS417_25235 at 5468.812 kb on - strand, within PS417_25235 at 5468.838 kb on + strand, within PS417_25235 at 5468.839 kb on - strand, within PS417_25235 at 5468.839 kb on - strand, within PS417_25235 at 5468.923 kb on - strand, within PS417_25235 at 5468.932 kb on - strand, within PS417_25235 at 5468.962 kb on - strand, within PS417_25235 at 5468.975 kb on - strand, within PS417_25235 at 5469.015 kb on + strand, within PS417_25235 at 5469.016 kb on - strand, within PS417_25235 at 5469.016 kb on - strand, within PS417_25235 at 5469.016 kb on - strand, within PS417_25235 at 5469.016 kb on - strand, within PS417_25235 at 5469.133 kb on - strand, within PS417_25235 at 5469.188 kb on + strand, within PS417_25235 at 5469.188 kb on + strand, within PS417_25235 at 5469.189 kb on - strand, within PS417_25235 at 5469.225 kb on + strand, within PS417_25235 at 5469.348 kb on + strand at 5469.349 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction JP1 MOI 1 remove 5,467,658 + PS417_25225 0.54 +1.7 5,467,658 + PS417_25225 0.54 -1.4 5,467,802 - PS417_25225 0.88 +1.4 5,468,505 + +0.1 5,468,506 - -4.0 5,468,548 + -1.6 5,468,587 - -1.2 5,468,587 - +3.0 5,468,591 - -0.9 5,468,616 + -1.7 5,468,616 + -0.7 5,468,617 - -2.7 5,468,617 - -2.0 5,468,617 - +0.5 5,468,700 - +1.6 5,468,700 - PS417_25235 0.12 -1.0 5,468,745 + PS417_25235 0.18 -0.3 5,468,745 + PS417_25235 0.18 -0.4 5,468,745 + PS417_25235 0.18 -0.5 5,468,745 + PS417_25235 0.18 -0.4 5,468,745 + PS417_25235 0.18 +1.0 5,468,746 - PS417_25235 0.18 +0.1 5,468,811 + PS417_25235 0.27 -1.7 5,468,812 - PS417_25235 0.27 -1.5 5,468,812 - PS417_25235 0.27 -1.2 5,468,838 + PS417_25235 0.31 -1.5 5,468,839 - PS417_25235 0.31 -0.6 5,468,839 - PS417_25235 0.31 +0.1 5,468,923 - PS417_25235 0.43 -0.2 5,468,932 - PS417_25235 0.44 +1.1 5,468,962 - PS417_25235 0.48 +1.4 5,468,975 - PS417_25235 0.50 -1.2 5,469,015 + PS417_25235 0.56 +0.6 5,469,016 - PS417_25235 0.56 -1.7 5,469,016 - PS417_25235 0.56 -1.7 5,469,016 - PS417_25235 0.56 +0.9 5,469,016 - PS417_25235 0.56 -0.4 5,469,133 - PS417_25235 0.72 -0.1 5,469,188 + PS417_25235 0.80 +0.7 5,469,188 + PS417_25235 0.80 -1.9 5,469,189 - PS417_25235 0.80 -0.1 5,469,225 + PS417_25235 0.85 -1.4 5,469,348 + +1.6 5,469,349 - +0.4
Or see this region's nucleotide sequence