Experiment: JP1 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_02445 and PS417_02450 are separated by 108 nucleotides PS417_02450 and PS417_02455 are separated by 194 nucleotides PS417_02455 and PS417_02460 overlap by 4 nucleotides
PS417_02445: PS417_02445 - GTPase RsgA, at 560,128 to 561,159
_02445
PS417_02450: PS417_02450 - oligoribonuclease, at 561,268 to 561,810
_02450
PS417_02455: PS417_02455 - putative transporter, required for glycine utilization <small>(from data)</small>, at 562,005 to 562,619
_02455
PS417_02460: PS417_02460 - epoxyqueuosine reductase, at 562,616 to 563,680
_02460
Position (kb) 561
562 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 561.192 kb on + strand at 561.209 kb on + strand at 561.240 kb on + strand at 561.241 kb on - strand at 561.241 kb on - strand at 561.241 kb on - strand at 562.020 kb on + strand at 562.020 kb on + strand at 562.447 kb on - strand, within PS417_02455 at 562.480 kb on - strand, within PS417_02455 at 562.480 kb on - strand, within PS417_02455 at 562.482 kb on - strand, within PS417_02455 at 562.482 kb on - strand, within PS417_02455 at 562.494 kb on + strand, within PS417_02455 at 562.600 kb on - strand at 562.618 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction JP1 MOI 1 remove 561,192 + +2.6 561,209 + -0.1 561,240 + -1.0 561,241 - +0.7 561,241 - -0.9 561,241 - -0.3 562,020 + -1.8 562,020 + -0.1 562,447 - PS417_02455 0.72 +0.9 562,480 - PS417_02455 0.77 -4.6 562,480 - PS417_02455 0.77 -0.6 562,482 - PS417_02455 0.78 +0.4 562,482 - PS417_02455 0.78 +0.2 562,494 + PS417_02455 0.80 +0.7 562,600 - +2.4 562,618 - -2.1
Or see this region's nucleotide sequence