Experiment: JP1 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_02440 and PS417_02445 are separated by 51 nucleotides PS417_02445 and PS417_02450 are separated by 108 nucleotides PS417_02450 and PS417_02455 are separated by 194 nucleotides
PS417_02440: PS417_02440 - flagellar motor protein MotB, at 559,057 to 560,076
_02440
PS417_02445: PS417_02445 - GTPase RsgA, at 560,128 to 561,159
_02445
PS417_02450: PS417_02450 - oligoribonuclease, at 561,268 to 561,810
_02450
PS417_02455: PS417_02455 - putative transporter, required for glycine utilization <small>(from data)</small>, at 562,005 to 562,619
_02455
Position (kb) 560
561
562 Strain fitness (log2 ratio) -2
-1
0
1
2 at 559.231 kb on + strand, within PS417_02440 at 559.232 kb on - strand, within PS417_02440 at 559.676 kb on - strand, within PS417_02440 at 559.968 kb on + strand, within PS417_02440 at 559.969 kb on - strand, within PS417_02440 at 560.085 kb on + strand at 560.132 kb on + strand at 560.132 kb on + strand at 561.192 kb on + strand at 561.209 kb on + strand at 561.240 kb on + strand at 561.241 kb on - strand at 561.241 kb on - strand at 561.241 kb on - strand at 562.020 kb on + strand at 562.020 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction JP1 MOI 1 remove 559,231 + PS417_02440 0.17 +2.4 559,232 - PS417_02440 0.17 +2.4 559,676 - PS417_02440 0.61 +2.4 559,968 + PS417_02440 0.89 +0.1 559,969 - PS417_02440 0.89 -1.2 560,085 + +0.4 560,132 + -2.5 560,132 + -0.8 561,192 + +2.6 561,209 + -0.1 561,240 + -1.0 561,241 - +0.7 561,241 - -0.9 561,241 - -0.3 562,020 + -1.8 562,020 + -0.1
Or see this region's nucleotide sequence