Experiment: JP1 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_02420 and PS417_02425 are separated by 15 nucleotides PS417_02425 and PS417_02430 are separated by 57 nucleotides PS417_02430 and PS417_02435 are separated by 166 nucleotides
PS417_02420: PS417_02420 - phosphatidylserine decarboxylase, at 554,751 to 555,611
_02420
PS417_02425: PS417_02425 - thiosulfate sulfurtransferase, at 555,627 to 556,442
_02425
PS417_02430: PS417_02430 - histidine kinase, at 556,500 to 558,035
_02430
PS417_02435: PS417_02435 - flagellar motor protein MotA, at 558,202 to 559,053
_02435
Position (kb)
556
557
558
559 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 555.622 kb on - strand at 555.683 kb on - strand at 555.683 kb on - strand at 555.724 kb on + strand, within PS417_02425 at 555.725 kb on - strand, within PS417_02425 at 555.986 kb on - strand, within PS417_02425 at 556.115 kb on - strand, within PS417_02425 at 556.193 kb on - strand, within PS417_02425 at 556.193 kb on - strand, within PS417_02425 at 556.193 kb on - strand, within PS417_02425 at 556.557 kb on - strand at 556.775 kb on + strand, within PS417_02430 at 556.776 kb on - strand, within PS417_02430 at 556.778 kb on + strand, within PS417_02430 at 556.779 kb on - strand, within PS417_02430 at 556.779 kb on - strand, within PS417_02430 at 556.973 kb on + strand, within PS417_02430 at 557.049 kb on + strand, within PS417_02430 at 557.049 kb on + strand, within PS417_02430 at 557.049 kb on + strand, within PS417_02430 at 557.050 kb on - strand at 557.885 kb on + strand at 557.961 kb on + strand at 557.961 kb on + strand at 557.962 kb on - strand at 557.962 kb on - strand at 558.110 kb on + strand at 558.111 kb on - strand at 558.136 kb on + strand at 558.136 kb on + strand at 558.137 kb on - strand at 558.137 kb on - strand at 558.156 kb on - strand at 558.164 kb on + strand at 558.164 kb on + strand at 558.164 kb on + strand at 558.165 kb on - strand at 558.165 kb on - strand at 558.190 kb on + strand at 558.190 kb on + strand at 558.190 kb on + strand at 558.190 kb on + strand at 558.190 kb on + strand at 558.191 kb on - strand at 558.191 kb on - strand at 558.191 kb on - strand at 558.201 kb on + strand at 558.201 kb on + strand at 558.202 kb on - strand at 558.208 kb on - strand at 558.254 kb on - strand at 558.271 kb on - strand at 558.422 kb on - strand, within PS417_02435 at 558.446 kb on - strand, within PS417_02435 at 558.571 kb on + strand, within PS417_02435 at 558.571 kb on + strand, within PS417_02435 at 558.571 kb on + strand, within PS417_02435 at 558.572 kb on - strand, within PS417_02435 at 558.583 kb on + strand, within PS417_02435 at 558.583 kb on + strand, within PS417_02435 at 558.712 kb on - strand, within PS417_02435 at 558.740 kb on + strand, within PS417_02435 at 558.914 kb on - strand, within PS417_02435
Per-strain Table
Position Strand Gene LocusTag Fraction JP1 MOI 1 remove 555,622 - -1.4 555,683 - +0.9 555,683 - -0.2 555,724 + PS417_02425 0.12 -2.0 555,725 - PS417_02425 0.12 -0.2 555,986 - PS417_02425 0.44 +0.0 556,115 - PS417_02425 0.60 -0.8 556,193 - PS417_02425 0.69 -0.2 556,193 - PS417_02425 0.69 -0.5 556,193 - PS417_02425 0.69 +1.0 556,557 - -0.4 556,775 + PS417_02430 0.18 -0.2 556,776 - PS417_02430 0.18 +2.0 556,778 + PS417_02430 0.18 -0.3 556,779 - PS417_02430 0.18 +0.5 556,779 - PS417_02430 0.18 -1.7 556,973 + PS417_02430 0.31 -2.2 557,049 + PS417_02430 0.36 +1.5 557,049 + PS417_02430 0.36 -0.9 557,049 + PS417_02430 0.36 -0.6 557,050 - -1.3 557,885 + +1.5 557,961 + +1.2 557,961 + -1.2 557,962 - +2.5 557,962 - +1.6 558,110 + -2.2 558,111 - +2.1 558,136 + -1.5 558,136 + +1.5 558,137 - +3.7 558,137 - +2.5 558,156 - +1.4 558,164 + +3.5 558,164 + +0.5 558,164 + +2.8 558,165 - +2.1 558,165 - +3.0 558,190 + +0.4 558,190 + -0.5 558,190 + -0.7 558,190 + +1.7 558,190 + +0.6 558,191 - +2.2 558,191 - +3.0 558,191 - +2.8 558,201 + +2.2 558,201 + +1.8 558,202 - +2.4 558,208 - +4.0 558,254 - +2.9 558,271 - +1.6 558,422 - PS417_02435 0.26 +2.0 558,446 - PS417_02435 0.29 +2.6 558,571 + PS417_02435 0.43 +2.8 558,571 + PS417_02435 0.43 +2.5 558,571 + PS417_02435 0.43 +1.7 558,572 - PS417_02435 0.43 +2.5 558,583 + PS417_02435 0.45 +2.8 558,583 + PS417_02435 0.45 +3.5 558,712 - PS417_02435 0.60 +2.5 558,740 + PS417_02435 0.63 +3.4 558,914 - PS417_02435 0.84 +1.9
Or see this region's nucleotide sequence