Experiment: JP1 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_23810 and PS417_23815 are separated by 431 nucleotides PS417_23815 and PS417_23820 overlap by 1 nucleotides PS417_23820 and PS417_23825 are separated by 16 nucleotides
PS417_23810: PS417_23810 - acetolactate synthase 3 catalytic subunit, at 5,151,863 to 5,153,587
_23810
PS417_23815: PS417_23815 - pseudouridine synthase, at 5,154,019 to 5,154,348
_23815
PS417_23820: PS417_23820 - hypothetical protein, at 5,154,348 to 5,155,082
_23820
PS417_23825: PS417_23825 - penicillin-binding protein, at 5,155,099 to 5,157,423
_23825
Position (kb) 5154
5155
5156 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 5153.487 kb on + strand at 5153.520 kb on + strand at 5153.520 kb on + strand at 5153.520 kb on + strand at 5153.521 kb on - strand at 5153.524 kb on + strand at 5153.526 kb on + strand at 5153.526 kb on + strand at 5153.527 kb on - strand at 5153.527 kb on - strand at 5153.577 kb on - strand at 5153.600 kb on + strand at 5153.613 kb on + strand at 5153.613 kb on + strand at 5153.640 kb on + strand at 5153.641 kb on - strand at 5153.643 kb on + strand at 5153.643 kb on + strand at 5153.649 kb on + strand at 5153.649 kb on + strand at 5153.649 kb on + strand at 5153.687 kb on + strand at 5153.775 kb on - strand at 5153.775 kb on - strand at 5154.185 kb on - strand, within PS417_23815 at 5154.313 kb on + strand, within PS417_23815 at 5154.313 kb on + strand, within PS417_23815 at 5154.313 kb on + strand, within PS417_23815 at 5154.313 kb on + strand, within PS417_23815 at 5154.314 kb on - strand, within PS417_23815 at 5154.314 kb on - strand, within PS417_23815 at 5154.314 kb on - strand, within PS417_23815 at 5154.314 kb on - strand, within PS417_23815 at 5154.528 kb on + strand, within PS417_23820 at 5154.528 kb on + strand, within PS417_23820 at 5154.742 kb on + strand, within PS417_23820 at 5154.742 kb on + strand, within PS417_23820 at 5154.742 kb on + strand, within PS417_23820 at 5154.743 kb on - strand, within PS417_23820 at 5154.743 kb on - strand, within PS417_23820 at 5154.743 kb on - strand, within PS417_23820 at 5154.743 kb on - strand, within PS417_23820 at 5154.870 kb on + strand, within PS417_23820 at 5155.055 kb on + strand at 5155.058 kb on + strand at 5155.059 kb on - strand at 5155.059 kb on - strand at 5155.199 kb on + strand at 5155.200 kb on - strand at 5155.691 kb on + strand, within PS417_23825 at 5155.692 kb on - strand, within PS417_23825 at 5155.844 kb on + strand, within PS417_23825 at 5155.865 kb on + strand, within PS417_23825 at 5155.976 kb on + strand, within PS417_23825 at 5155.977 kb on - strand, within PS417_23825
Per-strain Table
Position Strand Gene LocusTag Fraction JP1 MOI 1 remove 5,153,487 + +1.2 5,153,520 + +2.0 5,153,520 + -0.9 5,153,520 + +0.3 5,153,521 - +0.7 5,153,524 + -0.8 5,153,526 + -0.3 5,153,526 + -1.9 5,153,527 - -1.0 5,153,527 - -0.9 5,153,577 - -1.1 5,153,600 + +0.2 5,153,613 + -0.1 5,153,613 + +0.8 5,153,640 + +1.4 5,153,641 - -0.2 5,153,643 + +1.9 5,153,643 + +0.5 5,153,649 + -0.2 5,153,649 + +0.1 5,153,649 + -0.9 5,153,687 + +1.4 5,153,775 - -2.8 5,153,775 - -1.0 5,154,185 - PS417_23815 0.50 -3.3 5,154,313 + PS417_23815 0.89 -3.4 5,154,313 + PS417_23815 0.89 +1.2 5,154,313 + PS417_23815 0.89 -0.2 5,154,313 + PS417_23815 0.89 -1.0 5,154,314 - PS417_23815 0.89 +0.1 5,154,314 - PS417_23815 0.89 +1.3 5,154,314 - PS417_23815 0.89 -0.6 5,154,314 - PS417_23815 0.89 +1.0 5,154,528 + PS417_23820 0.24 +0.4 5,154,528 + PS417_23820 0.24 -0.6 5,154,742 + PS417_23820 0.54 -0.4 5,154,742 + PS417_23820 0.54 -1.2 5,154,742 + PS417_23820 0.54 -0.2 5,154,743 - PS417_23820 0.54 -1.4 5,154,743 - PS417_23820 0.54 -1.2 5,154,743 - PS417_23820 0.54 -3.1 5,154,743 - PS417_23820 0.54 -1.9 5,154,870 + PS417_23820 0.71 -1.6 5,155,055 + -0.6 5,155,058 + -0.1 5,155,059 - -3.6 5,155,059 - -1.1 5,155,199 + -3.5 5,155,200 - -1.3 5,155,691 + PS417_23825 0.25 -3.9 5,155,692 - PS417_23825 0.26 -1.2 5,155,844 + PS417_23825 0.32 -0.6 5,155,865 + PS417_23825 0.33 -1.9 5,155,976 + PS417_23825 0.38 -0.2 5,155,977 - PS417_23825 0.38 -2.3
Or see this region's nucleotide sequence