Experiment: JP1 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_02345 and PS417_02350 are separated by 120 nucleotides PS417_02350 and PS417_02355 are separated by 462 nucleotides
PS417_02345: PS417_02345 - 3-deoxy-D-manno-octulosonic acid transferase, at 535,078 to 536,355
_02345
PS417_02350: PS417_02350 - channel protein TolC, at 536,476 to 537,915
_02350
PS417_02355: PS417_02355 - thiamine biosynthesis protein ThiC, at 538,378 to 540,270
_02355
Position (kb) 536
537
538 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 536.401 kb on + strand at 536.401 kb on + strand at 536.402 kb on - strand at 536.465 kb on - strand at 536.467 kb on - strand at 536.467 kb on - strand at 536.467 kb on - strand at 536.477 kb on + strand at 536.478 kb on - strand at 536.480 kb on + strand at 536.555 kb on + strand at 536.645 kb on + strand, within PS417_02350 at 536.667 kb on - strand, within PS417_02350 at 536.667 kb on - strand, within PS417_02350 at 536.758 kb on + strand, within PS417_02350 at 536.759 kb on - strand, within PS417_02350 at 536.907 kb on - strand, within PS417_02350 at 536.907 kb on - strand, within PS417_02350 at 537.059 kb on + strand, within PS417_02350 at 537.060 kb on - strand, within PS417_02350 at 537.137 kb on + strand, within PS417_02350 at 537.137 kb on + strand, within PS417_02350 at 537.137 kb on + strand, within PS417_02350 at 537.255 kb on - strand, within PS417_02350 at 537.342 kb on - strand, within PS417_02350 at 537.810 kb on - strand at 537.966 kb on - strand at 538.012 kb on + strand at 538.012 kb on + strand at 538.013 kb on - strand at 538.102 kb on + strand at 538.102 kb on + strand at 538.103 kb on - strand at 538.349 kb on + strand at 538.579 kb on + strand at 538.597 kb on + strand, within PS417_02355 at 538.598 kb on - strand, within PS417_02355 at 538.667 kb on + strand, within PS417_02355 at 538.668 kb on - strand, within PS417_02355 at 538.813 kb on + strand, within PS417_02355 at 538.814 kb on - strand, within PS417_02355 at 538.814 kb on - strand, within PS417_02355 at 538.814 kb on - strand, within PS417_02355 at 538.850 kb on - strand, within PS417_02355
Per-strain Table
Position Strand Gene LocusTag Fraction JP1 MOI 1 remove 536,401 + +0.7 536,401 + -1.3 536,402 - +0.5 536,465 - +0.6 536,467 - -0.0 536,467 - +2.5 536,467 - +0.5 536,477 + -2.3 536,478 - -1.9 536,480 + -0.7 536,555 + -0.0 536,645 + PS417_02350 0.12 +0.8 536,667 - PS417_02350 0.13 +0.5 536,667 - PS417_02350 0.13 +2.8 536,758 + PS417_02350 0.20 +1.1 536,759 - PS417_02350 0.20 +1.0 536,907 - PS417_02350 0.30 +1.9 536,907 - PS417_02350 0.30 -2.5 537,059 + PS417_02350 0.40 +1.5 537,060 - PS417_02350 0.41 -0.2 537,137 + PS417_02350 0.46 +0.1 537,137 + PS417_02350 0.46 -1.5 537,137 + PS417_02350 0.46 -0.3 537,255 - PS417_02350 0.54 -1.3 537,342 - PS417_02350 0.60 +0.2 537,810 - +0.3 537,966 - +1.1 538,012 + +0.1 538,012 + +0.4 538,013 - -2.9 538,102 + +1.1 538,102 + +0.6 538,103 - +1.0 538,349 + +0.8 538,579 + +0.0 538,597 + PS417_02355 0.12 -1.1 538,598 - PS417_02355 0.12 +0.6 538,667 + PS417_02355 0.15 -2.9 538,668 - PS417_02355 0.15 -2.9 538,813 + PS417_02355 0.23 -1.3 538,814 - PS417_02355 0.23 +0.3 538,814 - PS417_02355 0.23 -0.8 538,814 - PS417_02355 0.23 -0.2 538,850 - PS417_02355 0.25 +0.6
Or see this region's nucleotide sequence