Experiment: JP1 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_23495 and PS417_23500 are separated by 2 nucleotides PS417_23500 and PS417_23505 are separated by 23 nucleotides PS417_23505 and PS417_23510 overlap by 8 nucleotides PS417_23510 and PS417_23515 are separated by 186 nucleotides
PS417_23495: PS417_23495 - GTP cyclohydrolase, at 5,085,861 to 5,086,415
_23495
PS417_23500: PS417_23500 - D-erythro-7,8-dihydroneopterin triphosphate epimerase, at 5,086,418 to 5,086,789
_23500
PS417_23505: PS417_23505 - cell division protein DedD, at 5,086,813 to 5,087,109
_23505
PS417_23510: PS417_23510 - HopJ type III effector protein, at 5,087,102 to 5,087,440
_23510
PS417_23515: PS417_23515 - thioredoxin reductase, at 5,087,627 to 5,088,589
_23515
Position (kb)
5087
5088 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 5086.122 kb on + strand, within PS417_23495 at 5086.122 kb on + strand, within PS417_23495 at 5086.123 kb on - strand, within PS417_23495 at 5086.123 kb on - strand, within PS417_23495 at 5086.123 kb on - strand, within PS417_23495 at 5086.179 kb on + strand, within PS417_23495 at 5086.179 kb on + strand, within PS417_23495 at 5086.179 kb on + strand, within PS417_23495 at 5086.180 kb on - strand, within PS417_23495 at 5086.180 kb on - strand, within PS417_23495 at 5086.180 kb on - strand, within PS417_23495 at 5086.180 kb on - strand, within PS417_23495 at 5086.413 kb on + strand at 5086.413 kb on + strand at 5086.414 kb on - strand at 5086.414 kb on - strand at 5086.414 kb on - strand at 5086.478 kb on + strand at 5086.478 kb on + strand, within PS417_23500 at 5086.478 kb on + strand, within PS417_23500 at 5086.478 kb on + strand, within PS417_23500 at 5086.479 kb on - strand, within PS417_23500 at 5086.479 kb on - strand, within PS417_23500 at 5086.479 kb on - strand, within PS417_23500 at 5086.596 kb on - strand, within PS417_23500 at 5086.952 kb on - strand, within PS417_23505 at 5086.952 kb on - strand, within PS417_23505 at 5087.186 kb on + strand, within PS417_23510 at 5087.187 kb on - strand, within PS417_23510 at 5087.192 kb on + strand, within PS417_23510 at 5087.192 kb on + strand, within PS417_23510
Per-strain Table
Position Strand Gene LocusTag Fraction JP1 MOI 1 remove 5,086,122 + PS417_23495 0.47 +0.5 5,086,122 + PS417_23495 0.47 +1.5 5,086,123 - PS417_23495 0.47 +0.7 5,086,123 - PS417_23495 0.47 +1.6 5,086,123 - PS417_23495 0.47 -1.2 5,086,179 + PS417_23495 0.57 -0.5 5,086,179 + PS417_23495 0.57 +0.3 5,086,179 + PS417_23495 0.57 +0.2 5,086,180 - PS417_23495 0.57 +2.8 5,086,180 - PS417_23495 0.57 +0.6 5,086,180 - PS417_23495 0.57 +2.0 5,086,180 - PS417_23495 0.57 -2.5 5,086,413 + -1.5 5,086,413 + +1.4 5,086,414 - +0.2 5,086,414 - +0.6 5,086,414 - +1.7 5,086,478 + +1.4 5,086,478 + PS417_23500 0.16 -0.2 5,086,478 + PS417_23500 0.16 +0.1 5,086,478 + PS417_23500 0.16 +0.1 5,086,479 - PS417_23500 0.16 -0.5 5,086,479 - PS417_23500 0.16 +1.1 5,086,479 - PS417_23500 0.16 +0.2 5,086,596 - PS417_23500 0.48 -0.2 5,086,952 - PS417_23505 0.47 -0.5 5,086,952 - PS417_23505 0.47 -1.6 5,087,186 + PS417_23510 0.25 +0.1 5,087,187 - PS417_23510 0.25 -1.2 5,087,192 + PS417_23510 0.27 +3.0 5,087,192 + PS417_23510 0.27 -0.5
Or see this region's nucleotide sequence