Strain Fitness in Pseudomonas simiae WCS417 around PS417_23140

Experiment: JP1 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPS417_23130 and PS417_23135 overlap by 8 nucleotidesPS417_23135 and PS417_23140 are separated by 41 nucleotidesPS417_23140 and PS417_23145 are separated by 21 nucleotides PS417_23130: PS417_23130 - dehydrogenase, at 5,014,267 to 5,015,418 _23130 PS417_23135: PS417_23135 - GTP-binding protein, at 5,015,411 to 5,016,052 _23135 PS417_23140: PS417_23140 - histidinol dehydrogenase, at 5,016,094 to 5,017,383 _23140 PS417_23145: PS417_23145 - 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase, at 5,017,405 to 5,018,514 _23145 Position (kb) 5016 5017 5018Strain fitness (log2 ratio) -1 0 1at 5015.451 kb on - strandat 5015.451 kb on - strandat 5015.451 kb on - strandat 5015.571 kb on - strand, within PS417_23135at 5015.926 kb on - strand, within PS417_23135

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Per-strain Table

Position Strand Gene LocusTag Fraction JP1 MOI 1
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5,015,451 - +1.5
5,015,451 - +0.0
5,015,451 - +0.8
5,015,571 - PS417_23135 0.25 +1.1
5,015,926 - PS417_23135 0.80 +1.5

Or see this region's nucleotide sequence