Experiment: JP1 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_23025 and PS417_23030 are separated by 136 nucleotides PS417_23030 and PS417_23035 are separated by 2 nucleotides PS417_23035 and PS417_23040 are separated by 179 nucleotides
PS417_23025: PS417_23025 - murein transglycosylase, at 4,988,622 to 4,990,082
_23025
PS417_23030: PS417_23030 - PTS glucose transporter subunit IIB, at 4,990,219 to 4,990,497
_23030
PS417_23035: PS417_23035 - D-trehalose PTS system, I, HPr, and IIA components <small>(from data)</small>, at 4,990,500 to 4,993,016
_23035
PS417_23040: PS417_23040 - D-trehalose outer membrane porin <small>(from data)</small>, at 4,993,196 to 4,994,416
_23040
Position (kb) 4990
4991
4992
4993
4994 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 4989.696 kb on + strand, within PS417_23025 at 4989.891 kb on + strand, within PS417_23025 at 4989.892 kb on - strand, within PS417_23025 at 4989.963 kb on + strand at 4989.963 kb on + strand at 4989.974 kb on + strand at 4989.974 kb on + strand at 4989.975 kb on - strand at 4989.997 kb on + strand at 4989.997 kb on + strand at 4990.080 kb on + strand at 4990.080 kb on + strand at 4990.081 kb on - strand at 4990.103 kb on - strand at 4990.108 kb on + strand at 4990.220 kb on + strand at 4990.871 kb on - strand, within PS417_23035 at 4991.145 kb on + strand, within PS417_23035 at 4991.200 kb on + strand, within PS417_23035 at 4991.200 kb on + strand, within PS417_23035 at 4991.201 kb on - strand, within PS417_23035 at 4992.386 kb on + strand, within PS417_23035 at 4992.418 kb on + strand, within PS417_23035 at 4992.822 kb on - strand at 4993.060 kb on - strand at 4993.060 kb on - strand at 4993.197 kb on + strand at 4993.332 kb on + strand, within PS417_23040 at 4993.332 kb on + strand, within PS417_23040 at 4993.333 kb on - strand, within PS417_23040 at 4993.333 kb on - strand, within PS417_23040 at 4993.333 kb on - strand, within PS417_23040 at 4993.338 kb on + strand, within PS417_23040 at 4993.338 kb on + strand, within PS417_23040 at 4993.338 kb on + strand, within PS417_23040 at 4993.339 kb on - strand, within PS417_23040 at 4993.339 kb on - strand, within PS417_23040 at 4993.561 kb on - strand, within PS417_23040 at 4993.664 kb on - strand, within PS417_23040 at 4993.666 kb on - strand, within PS417_23040 at 4993.800 kb on + strand, within PS417_23040 at 4993.800 kb on + strand, within PS417_23040 at 4993.801 kb on - strand, within PS417_23040 at 4993.801 kb on - strand, within PS417_23040 at 4993.806 kb on - strand, within PS417_23040 at 4993.806 kb on - strand, within PS417_23040 at 4993.876 kb on - strand, within PS417_23040 at 4993.920 kb on + strand, within PS417_23040 at 4993.920 kb on + strand, within PS417_23040 at 4993.980 kb on + strand, within PS417_23040 at 4993.981 kb on - strand, within PS417_23040 at 4993.996 kb on + strand, within PS417_23040 at 4994.009 kb on - strand, within PS417_23040 at 4994.016 kb on + strand, within PS417_23040 at 4994.016 kb on + strand, within PS417_23040
Per-strain Table
Position Strand Gene LocusTag Fraction JP1 MOI 1 remove 4,989,696 + PS417_23025 0.74 +1.3 4,989,891 + PS417_23025 0.87 +1.1 4,989,892 - PS417_23025 0.87 -0.7 4,989,963 + +0.3 4,989,963 + +0.4 4,989,974 + -0.5 4,989,974 + +0.9 4,989,975 - -1.3 4,989,997 + +0.5 4,989,997 + -0.3 4,990,080 + +0.2 4,990,080 + +0.3 4,990,081 - +0.8 4,990,103 - -0.0 4,990,108 + -1.3 4,990,220 + -0.8 4,990,871 - PS417_23035 0.15 +0.4 4,991,145 + PS417_23035 0.26 +2.1 4,991,200 + PS417_23035 0.28 +0.7 4,991,200 + PS417_23035 0.28 -0.2 4,991,201 - PS417_23035 0.28 +0.4 4,992,386 + PS417_23035 0.75 +0.6 4,992,418 + PS417_23035 0.76 +0.8 4,992,822 - -0.0 4,993,060 - +0.5 4,993,060 - +1.6 4,993,197 + +0.5 4,993,332 + PS417_23040 0.11 +0.2 4,993,332 + PS417_23040 0.11 +0.6 4,993,333 - PS417_23040 0.11 +0.5 4,993,333 - PS417_23040 0.11 +0.3 4,993,333 - PS417_23040 0.11 -1.5 4,993,338 + PS417_23040 0.12 +0.8 4,993,338 + PS417_23040 0.12 -0.3 4,993,338 + PS417_23040 0.12 +0.2 4,993,339 - PS417_23040 0.12 +0.4 4,993,339 - PS417_23040 0.12 +0.2 4,993,561 - PS417_23040 0.30 -0.5 4,993,664 - PS417_23040 0.38 -0.0 4,993,666 - PS417_23040 0.38 -0.5 4,993,800 + PS417_23040 0.49 -0.7 4,993,800 + PS417_23040 0.49 -0.1 4,993,801 - PS417_23040 0.50 +0.3 4,993,801 - PS417_23040 0.50 -2.8 4,993,806 - PS417_23040 0.50 -0.4 4,993,806 - PS417_23040 0.50 +0.5 4,993,876 - PS417_23040 0.56 +0.1 4,993,920 + PS417_23040 0.59 +1.5 4,993,920 + PS417_23040 0.59 -1.3 4,993,980 + PS417_23040 0.64 +0.7 4,993,981 - PS417_23040 0.64 -1.2 4,993,996 + PS417_23040 0.66 +0.1 4,994,009 - PS417_23040 0.67 -1.0 4,994,016 + PS417_23040 0.67 -1.0 4,994,016 + PS417_23040 0.67 -0.7
Or see this region's nucleotide sequence